Validity Test for a Yeast Two-Hybrid Assay to Screen for Estrogenic Activity, and Its Application to Insecticides and Disinfectants for Veterinary Use

Eguchi, Kiyohisa; Ozawa, Michihiro; Endoh, Y. S.; Nishikawa, J; Nishihara, Tsutomu; Goto, Kuniyasu; Yoshimura, Haruo

doi:10.1007/s00128-002-0181-8

Cited by 4 publications

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“…[910] Briefly, yeast cells expressing ER α and ER β were separately grown overnight at 30°C with shaking in a synthetic defined medium (SD) lacking tryptophan and leucine. Yeast cells were treated with 17 β -estradiol and the isolated compounds for 4 h at 30°C, and β -galactosidase activity was determined as follows: the growth of the yeast cells was monitored by measuring the turbidity at 600 nm.…”

Section: Methodsmentioning

confidence: 99%

Screening for estrogenic and antiestrogenic activities of plants growing in Egypt and Thailand

El-Halawany¹,

Dine²,

Chung³

et al. 2011

Phcog Res

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Background:There is a growing demand for the discovery of new phytoestrogens to be used as a safe and effective hormonal replacement therapy.Materials and Methods:The methanol extracts of 40 plants from the Egyptian and Thailand folk medicines were screened for their estrogen agonist and antagonist activities. The estrogenic and antiestrogenic effects of the tested extracts were carried out using the yeast two-hybrid assay system expressing ERα and ERβ. In addition, all the extracts were subjected to a naringinase treatment and retested for their estrogenic activity.Results:The methanol extracts of Derris reticulata and Dracaena lourieri showed the most potent estrogenic activity on both estrogen-receptor subtypes, while, the methanol extracts of Butea monosperma, Erythrina fusca, and Dalbergia candenatensis revealed significant estrogenic activity on ERβ only. Nigella sativa, Sophora japonica, Artabotrys harmandii, and Clitorea hanceana showed estrogenic effect only after naringinase treatment. The most potent antiestrogenic effect was revealed by Aframomum melegueta, Dalbergia candenatensis, Dracena loureiri, and Mansonia gagei.

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Section: Methodsmentioning

confidence: 99%