Valine uptake in the tap root of sugar beet: a comparative analysis with sucrose uptake

Michonneau, Philippe; Roblin, Gabriel; Bonmort, Janine; Fleurat‐Lessard, Pierrette

doi:10.1016/j.jplph.2004.02.005

Cited by 9 publications

(14 citation statements)

References 69 publications

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“…In order to verify that the different inhibitors did not act indirectly through a modification of the plasma membrane H + -ATPase activity, which energizes the H + co-transport of sugars and amino acids, we first assayed CB, CD and PH on the H + fluxes occurring in the plant tissues. As previously shown, a continuous acidification over a period of 5 h was observed in the incubation medium of root quarters, the pH decreasing from 6.3 to 5.5 (Michonneau et al, 2004). A similar observation has been made in the incubation medium of leaf tissues (Delrot, 1981).…”

Section: Effect Of Cytochalasin B Cytochalasin D and Phalloidin Osupporting

confidence: 86%

“…Taken together, our results indicated that CB, CD, and PH did not affect the uptake of sucrose and valine at the concentration used through a side effect by acting on the H + -ATPase, but achieved their inhibitory effect by acting directly on a physiological process that regulates the activity of the transporters. Previous studies have shown that the uptake of sucrose and amino acids occurs through an active process mediated by plasma membrane carriers which used an H + -substrate co-transport mechanism in leaf cells (Bush, 1989;Delrot et al, 1980;Lemoine & Delrot, 1989) and tap root cells (Michonneau et al, 2004). The presence of carriers supports the idea that nutrient fluxes are apoplastic (without ruling out the symplastic way).…”

Section: Effect Of Cytochalasin B Cytochalasin D and Phalloidin Omentioning

confidence: 58%

“…When measuring the uptake of sucrose and valine, PMVs were placed in an “energized” state following resuspension in 50 mM sodium phosphate (pH 5.5), containing 10 μM valinomycin (condition creating pH and gradients) (Lemoine et al, 1991). Uptake assays were carried out as previously described (Michonneau et al, 2004). Briefly, uptake was initiated by rapidly mixing vesicles with the incubation medium containing 1 mM [U‐ 14 C]sucrose (final activity: 27 kBq ml −1 ) and 1 mM [ 3 H]valine (final activity: 41 kBq ml −1 ).…”

Section: Methodsmentioning

confidence: 99%

“…The assays on taproot tissues were carried out as previously described (Michonneau et al, 2004). Tissue cylinders of 12 mm diameter were taken from the root cortex using a cork borer.…”

Section: Transport Assays In Vivomentioning

confidence: 99%

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Implication of actin in the uptake of sucrose and valine in the tap root and leaf of sugar beet

Michonneau

Fleurat‐Lessard

Cantereau³

et al. 2021

Physiologia Plantarum

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Actin microfilaments (F-actin) are major components of the cytoskeleton essential for many cellular dynamic processes (vesicle trafficking, cytoplasmic streaming, organelle movements). The aim of this study was to examine whether cortical actin microfilaments might be implicated in the regulation of nutrient uptake in root and leaf cells of Beta vulgaris. Using antibodies raised against actin and the AtSUC1 sucrose transporter, immunochemical assays demonstrated that the expression of actin and a sucrose transporter showed different characteristics, when detected on plasma membrane vesicles (PMVs) purified from roots and from leaves. The in situ immunolabeling of actin and AtSUC1 sites in PMVs and tissues showed their close proximity to the plasma membrane. Using co-labeling in protoplasts, actin and sucrose transporters were localized along the internal border and in the outermost part of the plasma membrane, respectively. This respective membrane co-localization was confirmed on PMVs and in tissues using transmission electronic microscopy. The possible functional role of actin in sucrose uptake (and valine uptake, comparatively) by PMVs and tissues from roots and leaves was examined using the pharmacological inhibitors, cytochalasin B (CB), cytochalasin D (CD), and phalloidin (PH). CB and CD inhibited the sucrose and valine uptake by root tissues in a concentration-dependent manner above 1 μM, whereas PH had no such effect. Comparatively, the toxins inhibited the sucrose and valine uptake in leaf discs to a lesser extent. The inhibition was not due to a hindering of the proton pumping and H + -ATPase catalytic activity determined in PMVs incubated in presence of these toxins. | INTRODUCTIONThe plant cytoskeleton is generally considered to consist of distinct components, for example, microtubules, microfilaments, and intermediary filaments. The microfilament system has been well characterized in plant cells. In particular, actin microfilaments (MFs or F-actin) are highly organized and major components of the cytoskeleton, and are essential for many cellular processes, including division, expansion, and differentiation (Higaki, Sano, & references therein, Hussey et al., 2006, and references therein). Further, dynamic processes, such as vesicle trafficking, cytoplasmic streaming, and organelle movements, are also controlled by MFs (Kandasamy & Meagher, 1999;Van Gestel et al., 2002). Moreover, the actin cytoskeleton is implicated in plant defense against pathogens (Hardham et al., 2007;Tian et al., 2009), and MF modification is also observed during elicitor-induced vacuolar disintegration in programmed cell death (Higaki, Goh, et al., 2007). Plants express multiple actin isoforms, which are dependent on tissues, organs, and stage of development, suggesting that individual isoforms play specific roles in cells, presumably through interactions with specific actin binding proteins (Kijima et al., 2016). Actin and some of the associated proteins are well conserved in plants and animals (Abu-Abied et al., 2006).

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Section: Effect Of Cytochalasin B Cytochalasin D and Phalloidin Osupporting

confidence: 86%

Section: Effect Of Cytochalasin B Cytochalasin D and Phalloidin Omentioning

confidence: 58%

Section: Methodsmentioning

confidence: 99%

“…The assays on taproot tissues were carried out as previously described (Michonneau et al, 2004). Tissue cylinders of 12 mm diameter were taken from the root cortex using a cork borer.…”

Section: Transport Assays In Vivomentioning

confidence: 99%

See 2 more Smart Citations

Implication of actin in the uptake of sucrose and valine in the tap root and leaf of sugar beet

Michonneau

Fleurat‐Lessard

Cantereau³

et al. 2021

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

show abstract

“…Individual amino acids include the twenty structural amino acids involved in the synthesis of proteins and non-protein amino acids, which are abundant in some plant species (Vranova et al, 2011). These amino acids are adsorbed by both roots and leaves and then translocated into the plant (Watson and Fowden, 1975;Soldal and Nissen, 1978;Michonneau et al, 2004;Zhang et al, 2015). Several studies testing the action of protein hydrolysates in plants, have demonstrated that their first effect was the stimulation of root and leaf biomass (Zhang et al, 2003;Schiavon et al, 2008;Ertani et al, 2009).…”

Section: Protein-based Biostimulantsmentioning

confidence: 99%

Plant biostimulants: physiological responses induced by protein hydrolyzed-based products and humic substances in plant metabolism

Nardi

Pizzeghello

Schiavon

et al. 2016

Sci. agric. (Piracicaba, Braz.)

297

196

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In recent years, the use of biostimulants in sustainable agriculture has been growing. Biostimulants can be obtained from different organic materials and include humic substances (HS), complex organic materials, beneficial chemical elements, peptides and amino acids, inorganic salts, seaweed extracts, chitin and chitosan derivatives, antitranspirants, amino acids and other N-containing substances. The application of biostimulants to plants leads to higher content of nutrients in their tissue and positive metabolic changes. For these reasons, the development of new biostimulants has become a focus of scientific interest. Among their different functions, biostimulants influence plant growth and nitrogen metabolism, especially because of their content in hormones and other signalling molecules. A significant increase in root hair length and density is often observed in plants treated with biostimulants, suggesting that these substances induce a "nutrient acquisition response" that favors nutrient uptake in plants via an increase in the absorptive surface area. Furthermore, biostimulants positively influence the activity and gene expression of enzymes functioning in the primary and secondary plant metabolism. This article reviews the current literature on two main classes of biostimulants: humic substances and protein-based biostimulants. The characteristic of these biostimulants and their effects on plants are thoroughly described.

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Methods of collection of plant root exudates in relation to plant metabolism and purpose: A review

Vranová

Rejšek

Skene

et al. 2013

Z. Pflanzenernähr. Bodenk.

181

111

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The aim of this work is to review the current knowledge on the effects of plant metabolism (C3, C4, and CAM) on root exudation and on the methods of exudate collection as well as the use of such exudates for analyses, testing of microbial response, degradation of pollutants, enzymatic activities, and occurrence of allelochemicals. We examine the advantages and disadvantages of each method as related to the downstream use of the exudates. The use of continuous percolation of solid cultivation medium with adjustment of nutrient‐solution strength appears to be a promising methodology for the determination of root exudation rates and qualitative composition of exuded compounds. The method mimics rhizosphere conditions, minimizing the artificial accumulation of compounds, alteration of plasma‐membrane permeability, ATPase activity, and the impacts of inhibitors or stimulators of root enzymes. Of particular significance is the fact that the adjustment of strength of nutrient solution and percolation enables universal and also long‐term use of the method, allowing high exudation yield by minimizing influx and maximizing efflux rates of exuded compounds at high nutrient‐solution strength. Furthermore, it facilitates assessment of the effect on soil microbial populations and their ability to degrade pollutants. Enzymatic activities can be assessed when a low strength of nutrient solution is used, with percolation of the exudates directly into tested soils. Composition of root exudates, regulation of root enzymes, and plant response to nutrient deficiency can be assessed by measuring net efflux or influx rates. The impact of heavy metals and other type of mechanical, chemical, and biological stresses differs according to the type of plant metabolism. This has significant consequences on transformations in plant communities, both structurally and functionally, and impacts upon crop nutrition, with respect to global climate change, and the use of plants for phytoremediation purposes. Understanding the effects of different types of plant metabolism on root exudation with respect to genetic regulation of synthetic pathways through root enzymes and transport systems presents an important direction for future research.

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Valine uptake in the tap root of sugar beet: a comparative analysis with sucrose uptake

Cited by 9 publications

References 69 publications

Implication of actin in the uptake of sucrose and valine in the tap root and leaf of sugar beet

Implication of actin in the uptake of sucrose and valine in the tap root and leaf of sugar beet

Plant biostimulants: physiological responses induced by protein hydrolyzed-based products and humic substances in plant metabolism

Methods of collection of plant root exudates in relation to plant metabolism and purpose: A review

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