Polyvinyl chloride remains one of the most prevalent polymers in the industry, yet its substantial environmental impact, attributed to its fossil origin, prompts the exploration of innovative solutions. Composites, particularly bio-composites, emerge as promising alternatives to mitigate the ecological footprint of PVC while enhancing its characteristics. This study addresses this concern by presenting the development of a bio-composite comprising 90% PVC and 10% biological filler derived from bovine horn, renowned for its high keratin content. The primary objective was to create an innovative, environmentally friendly, and sustainable material. To rigorously assess the properties and thermal stability of this bio-composite, a comparative thermogravimetric analysis was conducted against virgin PVC. The results reveal the superior thermal stability of the biocomposite compared to virgin PVC, particularly beyond 280℃. This enhancement is attributed to the substantial presence of keratin in the biological filler, constituting nearly 90% of the horn biomass. Notably, the observed mass loss in the bio-composite is lower than that of virgin PVC at temperatures exceeding 280℃. This research underscores the potential of bio-composites, specifically those incorporating bovine horn-derived filler, as promising alternatives to mitigate the ecological footprint of PVC while concurrently improving its thermomechanical characteristics. The innovative material developed in this study holds promise for sustainable applications in various industries, aligning with the growing demand for environmentally conscious alternatives.