Valorization of agro-industrial by-products for sustainable production of biosurfactant by Syncephalastrum racemosum UCP 1302

Abstract

The reutilization of agro-industrial by-products for obtaining of high-value added biosurfactants is a promising approach for minimizing the total production costs. In this context, this study aimed to evaluate the production of biosurfactant by the Mucoralean fungus Syncephalastrum racemosum UCP 1302, by bioconversion of renewable substrates: cassava wastewater (CWW), waste soybean oil (WSO) and corn steep liquor (CSL). For this, a 23 full-factorial design (FFD) was applied and the results showed the ability of this strain to produce biosurfactant in all conditions of the FFD, standing out the condition 7 due to the greatest reduction of surface tension (from 72 to 30.9 mN/m). Preliminary characterization showed the lipopeptide nature of the biomolecule, as well as its anionic character and critical micellar concentration (CMC) of 1.25 mg/ml. Biotensoactive demonstrated stability to variations of temperature, pH and NaCl concentrations, wettability in polyester textile and it was effective on reduction of viscosity of burned motor oil. Hence, S. racemosum showed excellent ability to produce biosurfactant by green bioconversion of low-cost substrates, making the bioprocess economical and enabling its biotechnological applications.

Eco-friendly production of biosurfactant by Mucor circinelloides UCP 0018 using agro-industrial substrates

“…According to the analysis done by Potential Zeta Meta 3.0 +, the BS showed anionic character (-17.4 mV), similar to others BS produced by Mucoralean fungi Pele, et al, 2019;Bione, et al, 2022). Anionic surfactants have a negative charge in their polar part, as well as strong foaming, detergent and wetting power.…”

“…Previously, Santiago et al (2021) related this strain as BS producer, because of the reduction of surface tension to 34.0 mN/m in medium formulated with Jatobá (Hymenaea stilbocarpa) husks and CSL. Several researches reported the effectiveness of CSL and CG as inductors for microbial BS production (Pele, et al, 2019;Mendonça, et al, 2021;Bione, et al, 2022). In this context, the statistical analysis verified the significant effect of the concentrations of both agro-industrial by-products on surface tension.…”

“…Previously, lower yields were reported for BS produced by Mucoralean fungi Rhizopus arrhizus UCP 1607 (1.74 g/L) (Pele, et al, 2019) and Syncephalastrum racemosum UCP 1302 (0.9 g/L) (Bione, et al, 2022).…”

“…The ionic charge of BS was investigated using 100 mg of the biomolecule solubilized in 5 ml of distilled water, using a Zeta ZM3-D-G potentiometer, Zeta Meter System 3.0+, and the direct images were recorded in a Zeta Meter video, San Francisco, CA, USA (Bione, et al, 2022).…”

Cassava wastewater valorization for the production of biosurfactants: surfactin, rhamnolipids, and mannosileritritol lipids

“…In more recent studies, Bione et al (2022) used 3.5% cassava wastewater (CW) as a culture medium supplemented with 6.5% waste soybean oil (WSO) and 6.5% corn steep liquor (CSL). Fermentation was made by bench-batch with Syncephalastrum racemosum UCP 1302 at 150 rpm agitation and 28 °C.…”