Valorization of Agro-Industrial Orange Peel By-Products through Fermentation Strategies

Gervasi, Teresa; Mandalari, Giuseppina

doi:10.3390/fermentation10050224

Cited by 2 publications

(1 citation statement)

References 92 publications

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“…Additionally, the production of carboxylic acids using orange peel waste was demonstrated, such as citric acid [9.2 g/L by Aspergillus niger ( Rivas et al, 2008 )], D -lactic acid [48.9 g/L using Lactobacillus delbrueckii ( De la Torre et al, 2019a )] or succinic acid [1.9 g/L using Fibrobacter succinogenes ( Li et al, 2010 )]. The product portfolio also encompasses the polymers xanthan ( Mohsin et al, 2018 ) and polyhydroxybutyrate (PHB) ( Davaritouchaee et al, 2023 ), in addition to volatile esters ( Lalou et al, 2013 ) and few other products ( Gervasi and Mandalari, 2024 ). Despite the rich nitrogen content of food wastes, which has been generally neglected, one of the biggest sectors of industrial biotechnology ( Wendisch et al, 2022 ), the production of the nitrogenous amino acids, has not yet received attention regarding the use of orange peel wastes.…”

Section: Introductionmentioning

confidence: 99%

Utilization of orange peel waste for sustainable amino acid production by Corynebacterium glutamicum

Junker,

Sariyar Akbulut,

Wendisch

2024

Front. Bioeng. Biotechnol.

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Oranges are the most processed fruit in the world–it is therefore apparent that the industrial production of orange juice generates large quantities of orange peel as a by-product. Unfortunately, the management of the orange peel waste leads to economic and environmental problems. Meanwhile, the use of sustainable raw materials for the production of bulk chemicals, such as amino acids, is becoming increasingly attractive. To address both issues, this study focused on the use of orange peel waste as a raw material for media preparation for the production of amino acids by engineered Corynebacterium glutamicum. C. glutamicum grew on pure orange peel hydrolysate (OPH) and growth was enhanced by the addition of a nitrogen source and a pH buffer. Inhibitory effects by the combination of high concentrations of OPH, (NH4)2SO4, and MOPS buffer in the wild-type strain (WT), were overcome in the tyrosine-producing engineered C. glutamicum strain AROM3. Genetic modifications that we identified to allow for improved growth rates under these conditions included the deletions of the vanillin dehydrogenase gene vdh, the ʟ-lactate dehydrogenase gene ldhA and the 19 genes comprising cluster cg2663-cg2686. A growth inhibiting compound present in high concentrations in the OPH is 5-(hydroxymethyl)furfural (HMF). We identified vdh as being primarily responsible for the oxidation of HMF to its acid 5-hydroxymethyl-2-furancarboxylic acid (HMFCA), as the formation of HMFCA was reduced by 97% upon deletion of vdh in C. glutamicum WT. In addition, we showed that growth limitations could be overcome by adjusting the media preparation, using a combination of cheap ammonia water and KOH for pH neutralization after acidic hydrolysis. Overall, we developed a sustainable medium based on orange peel waste for the cultivation of C. glutamicum and demonstrated the successful production of the exemplary amino acids ʟ-arginine, ʟ-lysine, ʟ-serine, ʟ-valine and ʟ-tyrosine.

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