Valorization of Crude Glycerol into Citric Acid and Malic Acid by <i>Yarrowia lipolytica</i>

Qian, Xiujuan; Xu, Ning; Jing, Yiwen; Song, Meng; Zhou, Jie; Dong, Weiliang; Xin, Fengxue; Zhang, Wenming; Jiang, Min; Ochsenreither, Katrin

doi:10.1021/acs.iecr.0c01723

Cited by 18 publications

(7 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Qian et al used Yarrowia lipolytica W29 to produce citric acid and malic acid in crude glycerol. The maximum concentration of citric acid was 9.87 g/L with the addition of a 10 g/L acetic acid salt solution in 50 g/L of glycerol, which was 14.7% higher than that without an acetic acid salt solution [36]. After 48 h of detoxification, furfural, HMF, and acetic acid were completely removed from the lignocellulosic dilute acid hydrolysate, which is suitable for fermentation systems that use sugar as a substrate.…”

Section: A Niger M13 Spores Biodetoxification Of Corncob Dilute Acid ...mentioning

confidence: 95%

Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger

Yin,

Wang,

et al. 2023

Fermentation

View full text Add to dashboard Cite

The biological detoxification of lignocellulose hydrolysate is an effective method through which to enhance microbial fermentation efficiency. In this study, an inhibitor-tolerant strain of A. niger (Aspergillus niger) was used for the biological detoxification of corncob hydrolysate. The results showed that A. niger M13 can tolerate a concentration of at least 7.50 ± 0.19 g/L of acetic acid, 1.81 ± 0.13 g/L of furfural, and 1.02 ± 0.10 g/L of HMF (5-Hydroxymethylfurfural). The spores had a higher detoxification efficiency than the mycelial pellets with a detoxification rate of 0.1566 g/L/h, 0.1125 g/L/h, and 0.015 g/L/h for acetic acid, furfural, and HMF, respectively. The cell preferentially consumed furfural, then HMF, before simultaneously degrading acetic acid and glucose. A. niger M13 spores could accumulate small amounts of citric acid directly from undetoxified hydrolysate at a concentration of about 6 g/L. Therefore, A. niger M13 can serve as an excellent biological detoxification strain and a potential citric acid fermenting strain when using undetoxified lignocellulosic hydrolysates.

show abstract

Section: A Niger M13 Spores Biodetoxification Of Corncob Dilute Acid ...mentioning

confidence: 95%

Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger

Yin,

Wang,

et al. 2023

Fermentation

View full text Add to dashboard Cite

show abstract

“…The French wild strain Y. lipolytica W29 can produce more than 100 g/L CA from glucose and glycerol [30]. In addition to these carbon sources, Y. lipolytica W29 produced CA from untreated crude glycerol [117], as well as from inexpensive, year-round nutrient sources obtained during the corn wet milling process, such as food-grade corn syrups and corn steep liquor [68]. However, when cultivated in glucose-enriched olive mill wastewater, the strain produced only 15.8 g/L CA with a yield (Y CA ) of 0.46 g/g [116].…”

Section: Wild-type Ca Producersmentioning

confidence: 99%

A Review on Citric Acid Production by Yarrowia lipolytica Yeast: Past and Present Challenges and Developments

Kamzolova

2023

Processes

View full text Add to dashboard Cite

The biosynthesis of citric acid (CA) and its derivatives is of great interest due to its wide range of applications in various manufacturing sectors. The fungus Aspergillus niger is mainly used for the commercial production of CA, using sucrose and molasses as the primary carbon sources. Since the 1960s, intensive research has been underway to introduce Yarrowia lipolytica yeast as an alternative to traditional fungal technology. This review discusses the practical uses of CA and its derivatives. Also, the challenges and developments that have led to efficient and green CA synthesis technologies using Y. lipolytica are outlined. The nutrient medium requirements and the use of various carbon sources, encompassing pure substrates and industry, agriculture, and food waste are considered. Additionally, the choice and improvement of strain producers, including efficient mutagenesis, genetic modification, and screening methods, are discussed.

show abstract

“…Therefore, the biotechnological process, in which the direct utilization of crude glycerol is possible, is very desirable. Up to now, crude glycerol was used as a substrate in citric acid, erythritol, α-ketoglutaric acid, lipids, and protein production by Y. lipolytica ( Dobrowolski et al, 2016 ; Cybulski et al, 2018 ; Qian et al, 2020 ; Rakicka-Pustułka et al, 2020 ). However, crude glycerol was never applied for KYNA biosynthesis by this yeast.…”

Section: Introductionmentioning

confidence: 99%

The microbial production of kynurenic acid using Yarrowia lipolytica yeast growing on crude glycerol and soybean molasses

Rakicka‐Pustułka

Ziuzia

Pierwoła

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Yarrowia lipolytica yeast are able to produce kynurenic acid—a very valuable compound acting as a neuroprotective and antioxidant agent in humans. The recent data proved the existence of the kynurenine biosynthesis pathway in this yeast cells. Due to this fact, the aim of this work was to enhance kynurenic acid production using crude glycerol and soybean molasses as cheap and renewable carbon and nitrogen sources. The obtained results showed that Y. lipolytica GUT1 mutants are able to produce kynurenic acid in higher concentrations (from 4.5 mg dm−3 to 14.1 mg dm−3) than the parental strain (3.6 mg dm−3) in the supernatant in a medium with crude glycerol. Moreover, the addition of soybean molasses increased kynurenic acid production by using wild type and transformant strains. The A-101.1.31 GUT1/1 mutant strain produced 17.7 mg dm−3 of kynurenic acid in the supernatant during 150 h of the process and 576.7 mg kg−1 of kynurenic acid in dry yeast biomass. The presented work proves the great potential of microbial kynurenic acid production using waste feedstock. Yeast biomass obtained in this work is rich in protein, with a low content of lipid, and can be a healthy ingredient of animal and human diet.

show abstract

Valorization of Crude Glycerol into Citric Acid and Malic Acid by Yarrowia lipolytica

Cited by 18 publications

References 45 publications

Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger

Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger

A Review on Citric Acid Production by Yarrowia lipolytica Yeast: Past and Present Challenges and Developments

The microbial production of kynurenic acid using Yarrowia lipolytica yeast growing on crude glycerol and soybean molasses

Contact Info

Product

Resources

About