Valorization of fatty acids-containing wastes and byproducts into short- and medium-chain length polyhydroxyalkanoates

Cruz, M.E.M.; Freitas, Filomena; Paiva, Alexandre; Mano, Francisca; Dionı́sio, Madalena; Ramos, A.M.; Reis, Maria A.M.

doi:10.1016/j.nbt.2015.05.005

Cited by 81 publications

(38 citation statements)

References 32 publications

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“…Polyhydroxyalkanoate quantification for microreactor assays was performed with slight modifications (Cruz et al, 2016). Briefly, 2-3 mg of dry cells was obtained from 3 ml of a culture of Caulobacter segnis with MM3 medium considering that a concentration of 0.7 g l À1 of CDW was reached (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…The fluorescence spectrometer used was Hitachi F7000 (Hitachi Europe, Krefeld, Germany) with k excitation = 545 nm and k emission = 598 nm. The protocol was adapted from a simple and highly sensitive method to detect PHA from growing colonies (Spiekermann et al, 1999;Cruz et al, 2016). Assays were performed in multiwell plates with volume of 200 ll and incubating at 30°C and 500 rpm for 24 h. The initial OD 600 of the cultures was set at 0.3, and the OD 600 at 24 h of each strain was adjusted to the lowest OD 600 obtained to normalized cell densities for RFU measurements.…”

Section: Screening With Nile Redmentioning

confidence: 99%

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In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

Bustamante

Segarra

Tortajada

et al. 2019

Microbial Biotechnology

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Summary Polyhydroxyalkanoates ( PHA s) are polyesters of microbial origin that can be synthesized by prokaryotes from noble sugars or lipids and from complex renewable substrates. They are an attractive alternative to conventional plastics because they are biodegradable and can be produced from renewable resources, such as the surplus of whey from dairy companies. After an in silico screening to search for ß‐galactosidase and PHA polymerase genes, several bacteria were identified as potential PHA producers from whey based on their ability to hydrolyse lactose. Among them, Caulobacter segnis DSM 29236 was selected as a suitable strain to develop a process for whey surplus valorization. This microorganism accumulated 31.5% of cell dry weight ( CDW ) of poly(3‐hydroxybutyrate) ( PHB ) with a titre of 1.5 g l −1 in batch assays. Moreover, the strain accumulated 37% of CDW of PHB and 9.3 g l −1 in fed‐batch mode of operation. This study reveals this species as a PHA producer and experimentally validates the in silico bioprospecting strategy for selecting microorganisms for waste re‐valorization.

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Section: Methodsmentioning

confidence: 99%

Section: Screening With Nile Redmentioning

confidence: 99%

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

Bustamante

Segarra

Tortajada

et al. 2019

Microbial Biotechnology

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“…are used as a substrate for industrial synthesis of PHAs (Mozejko and Ciesielski ; Cruz et al . ; Obruca et al . ).…”

Section: The Optimized Strategies Used In the Production Of Phasmentioning

confidence: 98%

“…Utilization of cellulosic waste such as wheat straw and bagasse proved to enhance the bioproduction of PHAs efficiently (Alva Munoz and Riley 2008;Cesario et al 2014); while various waste lipids containing oil extracted from spend coffee grounds, saponified waste palm oil, used cooking oil and etc. are used as a substrate for industrial synthesis of PHAs (Mozejko and Ciesielski 2013;Cruz et al 2015;Obruca et al 2015). In addition to the use of wastes, glucose is frequently used as a substrate for industrial synthesis of PHAs (Salgaonkar and Braganca 2015).…”

Section: Supplying Renewable Carbon Substratesmentioning

confidence: 99%

Recent strategies for efficient production of polyhydroxyalkanoates by micro-organisms

Liu

Zhang

et al. 2015

Lett Appl Microbiol

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Polyhydroxyalkanoates (PHAs) are polyesters accumulated by many bacteria under unbalanced growth conditions, and have been used to meet the various demands in areas of agriculture, medicine, and materials especially belong to a rapidly expanding area of biomedical research. Unfortunately, the high production cost than the traditional synthetic materials has greatly limited the wide application of PHA. Here, we systematically summarized recent progress in production of PHAs and a series of optimization strategies such as supplying renewable carbon substrates, developing better bacterial strains, optimization of fermentation processes, engineering new pathways and etc., were applied to reduce production cost, therefore providing many new ideas and methods for the production of PHAs in economically viable processes. This is believed to be a comprehensive report to show different strategies and methods for low-cost production of PHAs. Further studies are still needed to make PHAs more and more economically viable to meet a wide range of applicability.

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“…Several studies have evaluated various renewable carbon sources, such as canola oil (Rathinasabapathy et al 2013) and used cooking oil, which have demonstrated great biotechnological potential for the production of mcl-PHA (Cruz et al 2015). During the biosynthesis of PHA by Cupriavidus necator (formerly Wautersia eutropha > Ralstonia eutropha > Alcaligenes eutrophus) from fatty acids, López-Cuéllar et al (2011), Rathinasabapathy et al (2013) and Inomata et al (2014) demonstrated that C. necator was able to synthesize mcl-PHA using fructose and canola oil as substrates in a three stages fed-batch system, although it has been reported that the PHA synthase of C. necator is strictly specific for scl-PHA monomers (Slater et al 1992).…”

Section: Introductionmentioning

confidence: 99%

PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE

Quintanar-Gómez

Abreu-Corona

Zamuido-Pérez

et al. 2018

Rev. Int. Contam. Ambie.

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This study demonstrates that using beeswax hydrolyzates (Hw), short-or mediumchain-length polyhydroxyalkanoates (scl-mcl-PHA) can be obtained by a three stages fed-batch fermentation system. The fed batch fermentations were conducted with a strain of Cupriavidus necator. Glucose and ammonium sulphate as carbon and nitrogen sources, respectively, were used in a first and second culture stages. In the third stage (production of scl-mcl-PHA) a fresh medium was fed with Hw as a carbon source at a concentration of 5 g/L, obtaining 8.91 g/L of intracellular scl-mcl-PHA and 13.64 g/L of biomass, which resulted in 65.3 % w/w of scl-mcl-PHA. Using nuclear magnetic resonance, the presence of scl and mcl monomers, 3-hydroxybutyrate (3HB) and 3-hydroxydecanoate (3HD) was determined. The percentage of comonomer incorporation different to 3HB was 4.6 mol %. A glass transition temperature (Tg) of -50 ºC and a melting temperature (Tm) of 79.8 ºC were determined for the scl-mcl-PHA (3HB-co-3HDD).Palabras clave: biopolímeros, DSC, mcl-PHA, RMN, fermentación lote alimentado RESUMEN Este estudio demuestra que mediante el uso de hidrolizados de cera de abejas (Hw) pueden obtenerse polihidroxialcanoatos de cadena corta o media (scl-mcl-PHA), mediante un sistema de fermentación por lote alimentado de tres etapas. Las fermentaciones por lote alimentado se llevaron a cabo con una cepa de Cupriavidus necator. Se usaron glucosa y sulfato de amonio como fuentes de carbono y nitrógeno, respectivamente, en una primera y segunda etapas de cultivo. En la tercera etapa (producción de scl-mcl-PHA) se alimentó medio fresco con Hw como fuente de carbono a una concentración

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Valorization of fatty acids-containing wastes and byproducts into short- and medium-chain length polyhydroxyalkanoates

Cited by 81 publications

References 32 publications

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

Recent strategies for efficient production of polyhydroxyalkanoates by micro-organisms

PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE

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