Valorization of sugarcane biorefinery residues using fungal biocatalysis

Amini, Zeynab; Self, Rachel; Strong, Peter James; Speight, Robert; O’Hara, Ian M.; Harrison, Mark D.

doi:10.1007/s13399-021-01456-3

Cited by 9 publications

(11 citation statements)

References 134 publications

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“…Probiotics and enzymes can also be produced using solid state fermentations where the cells are grown on a solid substrate (Vandenberghe et al 2020). Although less established than submerged fermentations, solid-state processes can have process advantages and be useful when the solid substrate (fibre such as sugarcane bagasse, rice husks, or wheat bran) is also beneficially modified (pretreated to be more digestible) for inclusion in the final livestock feed (Amini et al 2021).…”

Section: Feed Supplement Productionmentioning

confidence: 99%

Platforms to accelerate biomanufacturing of enzyme and probiotic animal feed supplements: discovery considerations and manufacturing implications

et al. 2022

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Probiotics and enzymes are important components of the global livestock feed supplement market, which is expected to be approximately US$56 billion by 2027. They make essential contributions to animal health and productivity and are very important for on-farm economics, as well as feed supplement and bulk feed businesses. Despite the variety of on-market products, there remains a strong drive to develop new function or more effective enzymes (e.g. more active or stable) and probiotics (e.g. for specific health or nutrition requirements) that can be produced economically and commercialised to gain market share. Various large and established supplement development, manufacture and supply companies with highly refined, efficient and vertically integrated processes dominate the market. In contrast, many challenges exist for less established players, such as feed companies, large farming corporations, start-up companies and the research community, to develop and commercialise improved feed supplements. These less established players may have niche markets or needs or may have identified highly novel candidate products through basic or collaborative academia-industry applied research. In these situations, the path from discovery and development to a commercial product is unclear and likely to be very challenging. However, the risk of not progressing is that the value of research investments is not realised, or the needs of specific niche markets are not met. For these situations, new pathways to market based on rapid discovery, production (at various scales), and testing feedback loops, along with appropriate intellectual property management and clear regulatory strategies need to be established. To deliver these new pathways, it is essential to define key performance, production and economic criteria, have a rapid route from laboratory to pilot-scale manufacture and livestock feeding trials, and include all the necessary participants in the value chain from research development, manufacturing, distribution, and regulatory management to the end user. These issues are discussed with reference to the current state-of-the-art and our development of new pathways for a specific enzyme and probiotic based on efficient laboratory-to-market platforms. Although new supplements have been brought closer to market, challenges remain regarding scaling to commercial manufacture for new products without an established market.

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Section: Feed Supplement Productionmentioning

confidence: 99%

Platforms to accelerate biomanufacturing of enzyme and probiotic animal feed supplements: discovery considerations and manufacturing implications

et al. 2022

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Section: Introductionmentioning

confidence: 99%

“…Fungi are well-known for their capacity to secrete a broad range of cellulases with distinct proprieties and high titers – which are desired for the large application and demand for biotechnological purposes [3][4] [6]. Trichoderma reesei is the main filamentous fungi (hereafter called fungi) widely used to produce cellulases in industry owing to the high capacity of secretion – reaching up 100 g/l of cellulase [7][6] [5]. However, although T. reesei produce substantial amounts of exoglucanases and endoglucases, β-glucosidases production represents a low percentage of the total cellulases secreted, leading an incomplete saccharification process [8].…”

Section: Introductionmentioning

confidence: 99%

“…However, although T. reesei produce substantial amounts of exoglucanases and endoglucases, β-glucosidases production represents a low percentage of the total cellulases secreted, leading an incomplete saccharification process [8]. Therefore, due to the variety of applications and market expansion, there is still a need to identify and explore novel fungi capable of producing a high level of different cellulases at the same time with good activity and stability for efficiently digesting cellulose into glucose [4][6].…”

Section: Introductionmentioning

confidence: 99%

“…However, although T. reesei produce substantial amounts of exoglucanases and endoglucases, b-glucosidases production represents a low percentage of the total cellulases secreted, leading an incomplete saccharification process [8]. Therefore, due to the variety of applications and market expansion, there is still a need to identify and explore novel fungi capable of producing a high level of different cellulases at the same time with good activity and stability for efficiently digesting cellulose into glucose [4] [6]. Some T. harzianum strains have a high capacity to produce a large amount of all cellulases -including with high b-glucosidases activity [9][10] [11] [12] [13], representing a potential alternative to T. reesei for cellulase production on an industrial scale.…”

Section: Introductionmentioning

confidence: 99%

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An insight into cellulolytic capacity of the Trichoderma harzianum P49P11 revealed by omics approaches

Codima

Tomazetto

Persinoti

et al. 2022

Preprint

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Cellulases are a group of enzymes with several applications in biofuel production, and the paper, food, pharmaceutical, and chemical industries. Trichoderma harzianum P49P11 secrete all cellulases with high efficiency, representing an alternative to the current filamentous fungi in biotechnological industries. In this study, the cellulolytic mechanisms employed by the strain P49P11 to degrade crystalline cellulose in batch fermentation culture mode were elucidated by combining genome and secretome analysis. The strain P49P11 encodes nineteen cellulase genes from five different CAZyme families (GH5, GH6, GH7, GH12, and GH45), followed by several enzyme families for hemicellulose, pectin, and alpha- and beta-glucans degradation. The diverse CAZymes were also observed in the secretome, including cellulases, hemicellulases, and glucanases. In addition, beta-glucosidases and xylanase activities detected during the fermentation process validated our secretome analysis. Taken together, our results revealed all enzymatic machinery used by the T. harzianum P49P11 to degrade cellulose in batch fermentation mode.

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