2005
DOI: 10.1093/toxsci/kfi185
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Valproic Acid II: Effects on Oxidative Stress, Mitochondrial Membrane Potential, and Cytotoxicity in Glutathione-Depleted Rat Hepatocytes

Abstract: Oxidative stress has been associated with valproic acid (VPA) treatment, and mitochondrial dysfunction has been implicated in the pathogenesis of VPA-idiosyncratic hepatotoxicity. The present study investigated the effect of VPA and the role of GSH on oxidative stress, mitochondrial membrane potential, and toxicity in freshly isolated rat hepatocytes. Hepatocytes were isolated from Sprague-Dawley rats, and total levels of glutathione (GSH) reduced by pretreatment with a combination of L-buthionine sulfoximine … Show more

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Cited by 136 publications
(97 citation statements)
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“…Due to the fact that redox signaling is an important regulator of inflammation and fibrosis (Eddy 2000;Lei et al 2014), we used vit U, another antioxidant molecule, to protect VPA-induced renal injury. There are many studies showing that VPA elevates the ROS levels which are considered as a life-threatening side effect of VPA therapy (Tong et al 2005;Auinger et al 2009;Tung and Winn 2011). LPOs are generated as a result of ROS attack which leads to the destruction of cell membranes (Messaoudi et al 2009), and they are considered as key factor in VPA-mediated tissue damage (Chaudhary and Parvez 2012;Chaudhary et al 2014).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Due to the fact that redox signaling is an important regulator of inflammation and fibrosis (Eddy 2000;Lei et al 2014), we used vit U, another antioxidant molecule, to protect VPA-induced renal injury. There are many studies showing that VPA elevates the ROS levels which are considered as a life-threatening side effect of VPA therapy (Tong et al 2005;Auinger et al 2009;Tung and Winn 2011). LPOs are generated as a result of ROS attack which leads to the destruction of cell membranes (Messaoudi et al 2009), and they are considered as key factor in VPA-mediated tissue damage (Chaudhary and Parvez 2012;Chaudhary et al 2014).…”
Section: Discussionmentioning
confidence: 99%
“…For instance, VPA-induced ROS generation has been observed in cases of hepatotoxicity (Tong et al 2005), neurotoxicity (Auinger et al 2009), teratogenicity (Tung and Winn 2011), and also renal toxicity (Chaudhary et al 2014). Although it is a well-known fact that inflammation is regulated by redox signaling (Lei et al 2014) and fibrosis is induced by inflammation (Eddy 2000), to date, there has been no study explaining the pathogenesis mechanism of VPA-induced renal toxicity completely.…”
Section: Introductionmentioning
confidence: 99%
“…Rat hepatocytes were isolated by a two-step collagenase perfusion method (Seglen, 1993), as described previously (Tong et al, 2005a). Plating of hepatocytes (0.7 Â 10 6 cells/well), preparing the sandwich-culture configuration, and culturing of hepatocytes were performed, as reported earlier (Surendradoss et al, 2012).…”
Section: Methodsmentioning
confidence: 99%
“…The use of a positive control allows the correction of differences between the measurements, e.g., dye loading of the cells. FCCP was recommended previously as a positive control for the sensitive JC-1 flow cytometry method [21,[33][34][35][36]. The median fluorescence value for red (FL-2) was divided by the median fluorescence value for green (FL-1) obtained without FCCP.…”
Section: Patient Specimen Collectionmentioning
confidence: 99%