Value-added co-products from biomass of the diatoms Staurosirella pinnata and Phaeodactylum tricornutum

Microalgae are known producers of antioxidant and anti-inflammatory compounds, making them natural alternatives to be used as food and feed functional ingredients. This study aimed to valorise biomass and exploit new applications and commercial value for four commercially available microalgae: Isochrysis galbana, Nannochloropsis sp., Tetraselmis sp., and Phaeodactylum tricornutum. For that, five extracts were obtained: acetone (A), ethanol (E), water (W), ethanol:water (EW). The antioxidant capacity (ABTS•+/DPPH•/•NO/O2•−/ORAC-FL) and anti-inflammatory capacity (HBRC/COX-2) of the extracts were screened. The general biochemical composition (carbohydrates, soluble proteins, and lipids) and the main groups of bioactive compounds (carotenoids, phenolic compounds, and peptides) of extracts were quantified. The results of antioxidant assays revealed the potential of some microalgae extracts: in ABTS•+, Nannochloropsis sp. E and Tetraselmis sp. A, E, and P; in DPPH•, Tetraselmis sp. A and E; in •NO, P. tricornutum E and EW; in O2•−, Tetraselmis sp. W; and in ORAC-FL, I. galbana EW and P. tricornutum EW. Concerning anti-inflammatory capacity, P. tricornutum EW and Tetraselmis sp. W showed a promising HBRC protective effect and COX-2 inhibition. Hence, Tetraselmis sp. and P. tricornutum extracts seem to have potential to be incorporated as feed and food functional ingredients and preservatives.

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“…This was observed either in microalgae fish/shrimp feed formulations or using Tetraselmis sp. and P. tricornutum as food ingredients [5,40].…”

Section: Discussionmentioning

confidence: 99%

Potential of Microalgae Extracts for Food and Feed Supplementation—A Promising Source of Antioxidant and Anti-Inflammatory Compounds

Pagels

Amaro

Tavares

et al. 2022

Life

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“…Staurosirella pinnata was intensively cultivated on ten occasions in an indoor, column-photobioreactor (8L), and then harvested at the stationary phase. Clean frustules of S. pinnata were obtained from biomass remaining after cascade extractions for other potentially valuable co-products (Savio et al, 2020). Briefly, the pooled biomass was lyophilized, manually grinded and then extracted with a solution of milli-Q water and MetOH (20% v/v) for 2 hours at 45°C to obtain a crude extract.…”

Section: Frustule Preparationmentioning

confidence: 99%

“…Cultured diatoms can be considered as nano-factories, able to produce up to 10 6 frustules per mL (Wang and Seibert, 2017) which would be structurally identical with overall more predictable functions (Rogato and De Tommasi, 2020). In addition, biorefinery of diatoms would result in biomass from which different bioproducts could be extracted beforehand (Gilbert-Loṕez et al, 2017;Savio et al, 2020). The exhausted biomass at the end of cascade extractions, would be constituted by frustules ready for functionalization, improving the overall value of the biomass and the sustainability of the process.…”

Section: Introductionmentioning

confidence: 99%

“…The aim of this study, was to optimize the functionalization of frustules of the diatom Staurosirella pinnata (Ehrenberg) D.M.Williams & Round, obtained from the exhausted biomass of a diatom culture biorefinery (De Angelis et al, 2016;Savio et al, 2020) and to use them in the treatment of a wastewater contaminated with nickel. The main functionalization protocol parameters will be adjusted to best preserve the frustule porous ultrastructure and morphology whilst giving a homogeneous silane coating.…”

Section: Introductionmentioning

confidence: 99%

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Functionalization of Frustules of the Diatom Staurosirella pinnata for Nickel (Ni) Adsorption From Contaminated Aqueous Solutions

et al. 2022

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The structural characteristics of diatom cell walls (frustules) has led to their widespread use in diverse biotechnological applications, some of which can be further improved by surface chemical modification (functionalization). The use of coating agents can significantly increase surface binding capacity for target compounds. Frustules of the diatom Staurosirella pinnata used here were a by-product after applying a cascade-extraction process (for other products) to mass cultures. The protocol for the cleaning and functionalization of raw frustules using 3-Mercaptopropyl-trimethoxysilane and 3-Aminopropyl-trimethoxysilane was optimized and reported. Functionalization efficacy was observed using Electron Microscopy, Energy Dispersive X-ray Spectroscopy and Fourier-Transform Infrared Spectroscopy. Optimally functionalized frustules were evaluated for nickel removal from aqueous solutions. Incubations of 10 min, using 1 g/L of frustules, gave almost complete Ni removal with functionalized frustules compared to 3% removal by raw frustules. The proposed protocol represents a reproducible and efficient alternative for Ni removal from contaminated water.

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“…The success of P. tricornutum in large scale cultivation has been attributed to its robustness, adaptability and high growth rates both in indoor and outdoor culture systems, such as raceways and circular ponds, or tubular, column and flat-panel photobioreactors [10]. Besides the development and optimization of single product extractions, e.g., triacylglycerols for biodiesel or eicosatetraenoic acid, docosahexaenoic acid and fucoxanthin for nutraceuticals, 'biorefinery' approaches have also been implemented to valorize the single P. tricornutum biomass in a number of different products, with continuous downstream advancements in the alga exploitation [1,[11][12][13]. This is not paralleled by the upstream process steps, where several challenges still need to be overcome in order to maintain the long-term sustainability of commercial biomass exploitation [14].…”

Section: Introductionmentioning

confidence: 99%

Keeping Track of Phaeodactylum tricornutum (Bacillariophyta) Culture Contamination by Potentiometric E-Tongue

Savio

Natale

Paolesse

et al. 2021

Sensors

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The large-scale cultivation of microalgae provides a wide spectrum of marketable bioproducts, profitably used in many fields, from the preparation of functional health products and feed supplement in aquaculture and animal husbandry to biofuels and green chemistry agents. The commercially successful algal biomass production requires effective strategies to maintain the process at desired productivity and stability levels. Hence, the development of effective early warning methods to timely indicate remedial actions and to undertake countermeasures is extremely important to avoid culture collapse and consequent economic losses. With the aim to develop an early warning method of algal contamination, the potentiometric E-tongue was applied to record the variations in the culture environments, over the whole growth process, of two unialgal cultures, Phaeodactylum tricornutum and a microalgal contaminant, along with those of their mixed culture. The E-tongue system ability to distinguish the cultures and to predict their growth stage, through the application of multivariate data analysis, was shown. A PLS regression method applied to the E-tongue output data allowed a good prediction of culture growth time, expressed as growth days, with R2 values in a range from 0.913 to 0.960 and RMSEP of 1.97–2.38 days. Moreover, the SIMCA and PLS-DA techniques were useful for cultures contamination monitoring. The constructed PLS-DA model properly discriminated 67% of cultures through the analysis of their growth media, i.e., environments, thus proving the potential of the E-tongue system for a real time monitoring of contamination in microalgal intensive cultivation.

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Value-added co-products from biomass of the diatoms Staurosirella pinnata and Phaeodactylum tricornutum

Cited by 21 publications

References 48 publications

Potential of Microalgae Extracts for Food and Feed Supplementation—A Promising Source of Antioxidant and Anti-Inflammatory Compounds

Potential of Microalgae Extracts for Food and Feed Supplementation—A Promising Source of Antioxidant and Anti-Inflammatory Compounds

Functionalization of Frustules of the Diatom Staurosirella pinnata for Nickel (Ni) Adsorption From Contaminated Aqueous Solutions

Keeping Track of Phaeodactylum tricornutum (Bacillariophyta) Culture Contamination by Potentiometric E-Tongue

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