Value of a novel PGA-collagen tube on recurrent laryngeal nerve regeneration in a rat model

Suzuki, Hiroshi; Araki, Koji; Matsui, Toshiyasu; Tomifuji, Masayuki; Yamashita, Taku; Kobayashi, Yasushi; Shiotani, Akiko

doi:10.1002/lary.25750

Cited by 36 publications

(38 citation statements)

References 18 publications

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“…Slides were viewed on a Nikon microscope (Eclipse E200, Nikon, Melville, NY) and images captured with a Leica camera (DFC480, Leica, Buffalo Grove, IL). As previously described, myofiber diameter was evaluated by measuring the lesser fiber diameter using Image J software (NIH, Bethesda, MD) …”

Section: Methodsmentioning

confidence: 99%

Motor endplate‐expressing cartilage‐muscle implants for reconstruction of a denervated hemilarynx

Brookes¹,

Voytik‐Harbin

Zhang³

et al. 2018

The Laryngoscope

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Objective Tissue engineering of the larynx requires a complex, multiple tissue layer design. Additionally, spontaneous reinnervation of the larynx after recurrent laryngeal nerve (RLN) injury is often disorganized, resulting in subpar function. This study investigates use of tissue‐engineered cartilage and motor endplate‐expressing (MEE) tissue‐engineered skeletal muscle implants for laryngeal reconstruction and the promotion of organized reinnervation after RLN injury. Methods F344 rat primary muscle progenitor cells (MPCs) were isolated. Three‐dimensional muscle constructs were created by encapsulating MPCs in type I oligomeric collagen under passive tension. Constructs were then cultured in differentiation medium (MPC control constructs) or induced to form motor endplates (MEE constructs) with neurotrophic agents. Three‐dimensional cartilage constructs were created with adipose stem cells differentiated in chondrocyte medium. The muscle and cartilage constructs were implanted into surgically created myochondral defects in the F344 rat larynx with injured or intact (control) RLN. At 1‐, 3‐, and 6‐month timepoints, videolaryngoscopy, electromyography (EMG), histology, and immunohistochemistry were used to assess outcomes. Results At all timepoints, cartilage‐muscle implants were well integrated into host tissue. Functionally, there was increased vocal fold adduction and EMG activity in nerve‐injured rats treated with the MEE constructs when compared to those treated with the MPC control constructs. Motor endplate‐expressing constructs had increased myofiber cross‐sectional area compared to MPC control constructs. Conclusion Although our laboratory previously demonstrated that muscle and cartilage constructs could be used separately for hemilaryngeal reconstruction, this study suggests combining them with the modification of MEEs rather than MPCs, resulting in improved muscle recovery after recurrent laryngeal nerve injury. Level of Evidence NA Laryngoscope, 129:1293–1300, 2019

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Section: Methodsmentioning

confidence: 99%

Motor endplate‐expressing cartilage‐muscle implants for reconstruction of a denervated hemilarynx

Brookes¹,

Voytik‐Harbin

Zhang³

et al. 2018

The Laryngoscope

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show abstract

“…In previous studies that used a rat injured-RLN model, 12 or 15 weeks postoperatively was selected as the experimental period. 5,7,8,10 Our preliminary studies suggested that nerve regeneration from proximal to distal stumps could be confirmed by histological examination 8 weeks postoperatively, so this was the experimental period selected to investigate the relatively earlier effect of using RADA16-I hydrogel with a rat injured-RLN model rather than the experimental period described in previous reports.…”

Section: Laryngoscopymentioning

confidence: 99%

“…3 RLNs invaded by tumors require excision; therefore, RLN defects occur in such patients. Although various methods to regenerate defected RLNs have been reported, [4][5][6][7][8][9][10][11] no consistently effective treatments are currently available because they lead to insufficient functional recovery and misdirected innervation. 12 At present, preventing laryngeal muscle atrophy by maintaining nerve continuity is the only feasible treatment method for injured RLNs.…”

Section: Introductionmentioning

confidence: 99%

“…19 Such tubes have also been investigated in clinical studies and were reported to improve functional nerve regeneration. 20,21 With regard to treatments for defected RLNs, various types of nerve conduits have been investigated using animal models, [4][5][6][7][8][9][10][11] in which both the polyglycolic acid tubes 4,11 and silicone tubes 5 have been used. It has also been reported that a polyglycolic acid tube filled with collagen sponge was used as a scaffold for nerve regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…It has also been reported that a polyglycolic acid tube filled with collagen sponge was used as a scaffold for nerve regeneration. 7 In the present study, we focused on a self-assembling peptide (SAP) as the material with which to fill such tubes, in a similar way as a collagen sponge. A SAP is a synthetic chemical that can aggregate into nanofibers under physiological conditions.…”

Section: Introductionmentioning

confidence: 99%

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Recurrent laryngeal nerve regeneration using a self‐assembling peptide hydrogel

et al. 2019

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Objectives/Hypothesis To regenerate defected recurrent laryngeal nerves (RLNs), various methods have been developed. However, no consistently effective treatments are currently available because of their insufficient functional recovery. RADA16‐I, a self‐assembling peptide used clinically as a hemostat, reportedly supports neurite outgrowth and functional synapse formation in vitro. The purpose of this study was to investigate the effect of RADA16‐I hydrogels on transected RLNs in rats. Study Design Animal experiments with controls. Methods Fifteen adult rats were divided into the following three groups: RADA16‐I (+), RADA16‐I (−), and neurectomy. A 6‐mm gap of the left RLN was bridged using an 8‐mm silicone tube in the RADA16‐I (−) and RADA16‐I (+) groups. Subsequently, RADA16‐I hydrogel was injected into the tube in the RADA16‐I (+) group. The surgical incisions were closed without any further treatment in the neurectomy group. After 8 weeks, laryngoscopy and electrophysiological and histological examinations were performed to evaluate the effect of RADA16‐I on nerve regeneration and thyroarytenoid muscle atrophy. Results Although most rats in the three groups exhibited no improvements of their vocal fold movement, partial recovery was observed in one rat in the RADA16‐I (+) group. The neurofilament‐positive areas and the number of myelinated nerves in the RADA16‐I (+) group were significantly higher than in the RADA16‐I (−) group. The area of the left thyroarytenoid muscle in the RADA16‐I (+) group was significantly larger than that of the neurectomy group. Conclusions Our results suggested that RADA16‐I hydrogel was effective for RLN regeneration. Level of Evidence NA Laryngoscope, 130:2420–2427, 2020

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Lingual nerve revisited—A comprehensive review Part II: Surgery and radiology

Ono,

Nishioka,

Obata

et al. 2024

Clinical Anatomy

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The lingual nerve (LN) is a branch of the mandibular division of the fifth cranial nerve, the trigeminal nerve, arising in the infratemporal fossa. It provides sensory fibers to the mucous membranes of the floor of the mouth, the lingual gingiva, and the anterior two‐thirds of the tongue. Although the LN should rarely be encountered during routine and basic oral surgical procedures in daily dental practice, its anatomical location occasionally poses the risk of iatrogenic injury. The purpose of this section is to consider this potential LN injury risk and to educate readers about the anatomy of this nerve and how to treat it.

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Value of a novel PGA-collagen tube on recurrent laryngeal nerve regeneration in a rat model

Abstract: N/A. Laryngoscope, 126:E233-E239, 2016.

Cited by 36 publications

References 18 publications

Motor endplate‐expressing cartilage‐muscle implants for reconstruction of a denervated hemilarynx

Motor endplate‐expressing cartilage‐muscle implants for reconstruction of a denervated hemilarynx

Recurrent laryngeal nerve regeneration using a self‐assembling peptide hydrogel

Lingual nerve revisited—A comprehensive review Part II: Surgery and radiology

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