Value of Automatized Blood Culture Systems in the Diagnosis of Continuous Ambulatory Peritoneal Dialysis Peritonitis

Epidemiology of culture isolates from peritoneal dialysis peritonitis patients in southern India using an automated blood culture system to culture peritoneal dialysate

“…Here, we used BACTEC aerobic bottles (Becton Dickinson) for culture of PD fluid and encountered a high percentage of culture negativity (50%) in CAPD peritonitis cases which is much higher compared to the value of 20% as recommended by the International Society for Peritoneal Dialysis guidelines. Previous studies performed on various BACTEC systems have showed a much higher culture positivity of 70–75% as compared to our value of 50% 5,10,11 . Though we have used centrifugation and inoculation into BACTEC aerobic culture bottles, we were unable to increase the yield.…”

The Application Analysis of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Pathogenic Bacterium in Peritoneal Dialysis-Associated Peritonitis

“…However, failure to culture organisms (culture-negative peritonitis) remains common with current microbiological techniques. Culture-negative peritonitis remains a clinical issue as previous reports showed that the negative rates were between 13 and 55% [3,4]. However, culture-negative rates remain at 30-50% in our country, though the rates of a few big PD centers have fallen to about 25%, which are considerably higher than the standard level [5].…”

Consensus Guidelines for the Prevention and Treatment of Catheter-Related Infections and Peritonitis in Pediatric Patients Receiving Peritoneal Dialysis: 2012 Update

“…Two recent prospective studies also support the routine use of the broth culture technique compared with the water lysis technique (167,168). Preliminary organism identification by gram staining was 70.6% with the broth culture method, a rate significantly greater than the 17.6% achieved with the water lysis method.…”