Value of extended agitation and subculture of BACTEC NR 660 aerobic resin blood culture bottles for clinical yeast isolates

Prevost-Smith, Elena; Hutton, Nancy

doi:10.1128/jcm.30.12.3239-3242.1992

Cited by 15 publications

(3 citation statements)

References 13 publications

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“…In general, the mean times for recovery of yeasts from IS tubes and BP26 bottles were consistent with those previously published (3,4,9,19,21,23,29). The differences in mean recovery times generally were small and thus probably were not clinically important.…”

Section: Discussionsupporting

confidence: 89%

Controlled comparison of the BACTEC high-blood-volume fungal medium, BACTEC Plus 26 aerobic blood culture bottle, and 10-milliliter isolator blood culture system for detection of fungemia and bacteremia

et al. 1993

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The BACTEC high-blood-volume fungal medium (HBV-FM) (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) was compared with the Isolator (IS) tube and the BACTEC Plus 26 (BP26) blood culture bottle for the ability to recover fungi from the blood of adult patients suspected of having fungemia. A total of 6,836 blood culture sets that fulfilled criteria for inclusion in the study were received. Three separate comparisons were performed: 4,907 HBV-FM versus IS, 4,886 BP26 versus HBV-FM, and 4,949 BP26 versus IS. For the HBV-FM versus IS comparison, 218 isolates were recovered: 125 (57.3%) were bacteria and 93 (42.7%) were fungi. HBV-FM was comparable to IS for recovery of yeasts, but IS was superior for recovery of Histoplasma capsulatum (25 versus 0 isolates recovered [P < 0.001]). Growth of Torulopsis glabrata was detected earlier (P < 0.05) in HBV-FM bottles. For the BP26 versus HBV-FM comparison, 229 isolates were recovered: 161 (70.3%) were bacteria, and 68 (29.7%) were fungi. HBV-FM was superior for recovery of T. glabrata (P < 0.025) and all fungi combined (P < 0.025). There were no statistically significant differences in the speed of detection of microbial growth. For the BP26 versus IS comparison, 251 isolates were recovered: 165 (65.7%) were bacteria, and 86 (34.2%) were fungi. IS was superior for recovery of H. capsulatum (P < 0.001), T. glabrata (P < 0.05), and fungi other than H. capsulatum (P < 0.025).

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Section: Discussionsupporting

confidence: 89%

Controlled comparison of the BACTEC high-blood-volume fungal medium, BACTEC Plus 26 aerobic blood culture bottle, and 10-milliliter isolator blood culture system for detection of fungemia and bacteremia

et al. 1993

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Section: Discussionsupporting

confidence: 90%

BACTEC high-blood-volume fungal medium versus Isolator comparison misleading

Hunter¹

1993

J Clin Microbiol

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BACTEC High-Blood-Volume Fungal Medium versus Isolator Comparison Misleading Scientific investigation must be evaluated by using actual experimental conditions. Wilson et al. (4) mislead the reader by their global conclusion that BACTEC high-blood-volume fungal medium (HBV-FM) is equivalent and Bactec Plus 26 (BP26) is superior to Isolator. Tenets of microbial nutrition and experimental design were not followed, yet conclusions were drawn from flawed testing. Concerning microbial nutrition, the media and incubation conditions used for Isolator are not entirely those suggested by the manufacturer, and, as such, they create additional variables for simultaneous evaluation of both fungal and bacterial recovery. Regarding temperature, two of the four plates (Isolator) were incubated at 25°C (not optimal for growth of streptococci), whereas all BACTEC media were at 35°C. Regarding media, Why was Sabouraud dextrose agar selected rather than SABHI or BHI (suggested by manufacturer), both of which more closely resemble the formulation of BACTEC FM? Sabouraud (one plate) is neither conducive for bacterial growth nor recommended for recovery of fungi from clinical specimens such as blood (3). Although an option, Sabouraud was devised for dermatophytes (1). The type of Sabouraud (4% dextrose, pH 5.6) or Emmons medium (2% dextrose, pH neutral) used was not indicated. Media selected for recovery of fungi can influence yield (2), and one could postulate quite different results had optimal media been used. Dating back at least to the early 1940s, the literature is replete with examples of interrelationships between temperature, pH, nutritional requirements, and microbial growth. Such blatant omissions and inequalities by design are difficult to understand. Concerning the experimental design, basic principles of investigation, and especially those for blood cultures, dictate Controlled comparison of the BACTEC high-blood-volume fungal medium, BACTEC Plus 26 aerobic blood culture bottle, and 10-milliliter Isolator blood culture system for detection of fungemia and bacteremia. J. Clin. Microbiol. 31:865-871.

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“…In automated blood culture systems, most Candidapositive blood cultures flag within 2-3 days, and incubation for more than 5 days is not routinely required (Strong recommendation, Level II evidence). 49,50 In an attempt to reduce the time for identification, molecular and proteomic techniques (matrix-assisted laser-desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS)) applied to positive blood cultures are increasingly used. 51,52 Culture of sterile sites by direct aspiration may assist in distinguishing infection from colonisation.…”

Section: Recommendationsmentioning

confidence: 99%

Consensus guidelines for the diagnosis and management of invasive candidiasis in haematology, oncology and intensive care settings, 2021

Keighley

Cooley

Morris

et al. 2021

Internal Medicine Journal

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Patients with haematological malignancies, haemopoietic stem cell transplant recipients and patients requiring admission to intensive care settings are at high risk for invasive candidiasis (IC). Over the past decade, there has been increased reporting of nonalbicans species and fluconazole resistance in Australia. These guidelines provide updated evidence-based recommendations for the diagnosis and management of IC in adult and paediatric haematology, oncology and intensive care settings. Optimal pharmacological and non-pharmacological management are discussed. Recent studies strengthen the recommendation for an echinocandin agent as first-line therapy for high-risk patients with IC. Mortality benefit has also been demonstrated for nonpharmacological management, including removal of central venous catheters, infectious diseases consultation and use of care bundles. Healthcare facilities managing immunocompromised patient populations should therefore adopt implementation strategies for these multimodal interventions.

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Value of extended agitation and subculture of BACTEC NR 660 aerobic resin blood culture bottles for clinical yeast isolates

Cited by 15 publications

References 13 publications

Controlled comparison of the BACTEC high-blood-volume fungal medium, BACTEC Plus 26 aerobic blood culture bottle, and 10-milliliter isolator blood culture system for detection of fungemia and bacteremia

Controlled comparison of the BACTEC high-blood-volume fungal medium, BACTEC Plus 26 aerobic blood culture bottle, and 10-milliliter isolator blood culture system for detection of fungemia and bacteremia

BACTEC high-blood-volume fungal medium versus Isolator comparison misleading

Consensus guidelines for the diagnosis and management of invasive candidiasis in haematology, oncology and intensive care settings, 2021

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