Value of Nuclear DNA Ploidy Patterns in Patients with
Prostate Cancer after Radical Prostatectomy

Wirth, Manfred P.; Müller, Heimo; Manseck, Andreas; Müller, Joachim; Frohmüller, H.

doi:10.1159/000471708

Cited by 25 publications

(14 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several authors have proven the value of DNA ploidy analysis in the prognostication of prostatic cancer (3,11,19,21,23,31,35,37,40). Nativ et al (24) published a retrospective analysis of 146 cases of totally prostatectomized GI-3 pT, tumours and found a good correlation between DNA ploidy and prognosis.…”

Section: Hiiggman Et Almentioning

confidence: 99%

See 1 more Smart Citation

Pre- and Postoperative DNA Ploidy Patterns Correlated to Pt-Stage, Histological Grade and Tumour Volume in Total Prostatectomy Specimens

Häggman

Torre

Brändstedt

et al. 1994

Scandinavian Journal of Urology and Nephrology

View full text Add to dashboard Cite

Fifty-four consecutive patients with a preoperative diagnosis of localized prostatic cancer underwent total retropubic prostatectomy. The specimens were step-sectioned at 5 mm intervals. Volumes of invasive cancer (IC) and prostatic intraepithelial neoplasia (PIN) were calculated by computerized planimetry. From preoperative core and fine needle aspiration biopsies and from each prostatectomy specimen, multiple samples were taken from IC and PIN areas for DNA ploidy analysis. The study aimed to evaluate current biopsy sampling techniques as regards their suitability for DNA analysis and to assess the heterogeneity of DNA-ploidy and correlate the latter to postoperative pT-stage and tumour volume. When compared with the prostatectomy specimens, the preoperative assessment of non-diploid DNA patterns in biopsies had a sensitivity of 62% and a specificity of 86%. Samples from 24 surgical specimens contained non-diploid DNA in the main tumour and in one or several of the satellites. However, all these cases also contained diploid cell lines, both in the main tumour and in one or several of the satellites. Tumours with a volume exceeding 12 cc:s were non-diploid in 87.5% of cases (7/8). Tumours with volumes between 8 and 12 cc:s contained non-diploid foci in 50% of cases (2/4). Tumours smaller than 2 cc:s were non-diploid in 18% of cases (2/11). All non-diploid tumours were moderately or poorly differentiated. Of the non-diploid tumours, 96% (23/24) displayed capsular penetration versus 57% (17/30) of the diploid tumours (p < 0.0001). All PIN samples were made up of diploid cell lines.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Section: Hiiggman Et Almentioning

confidence: 99%

“…The assessment of DNA-ploidy in prostatic cancer has been shown to provide prognostic information (3,19,23,24,31,35,40). However, a substantial difficulty associated with this technique is the reported heterogeneity in the DNA-ploidy of prostatic cancer (13,19).…”

mentioning

confidence: 99%

Pre- and Postoperative DNA Ploidy Patterns Correlated to Pt-Stage, Histological Grade and Tumour Volume in Total Prostatectomy Specimens

Häggman

Torre

Brändstedt

et al. 1994

Scandinavian Journal of Urology and Nephrology

View full text Add to dashboard Cite

show abstract

“…• 10 level 2b studies were found that report on a statistically significant correlation of DNA-cytometric features with recurrence-free survival after radical prostatectomy in a multivariate-analysis [16,175- an univariate analysis [180,182,183] Table 4. [193] found the same after external radiation in a multivariate analysis.…”

Section: Methodologically Sufficient Papersmentioning

confidence: 99%

DNA-cytometric grading of prostate cancer Systematic review with descriptive data analysis

Böcking¹,

Tils²,

Schramm³

et al. 2014

Pathol Discov

View full text Add to dashboard Cite

Gleason-score <=6, assessed on core needle biopsies, is an essential prognostic parameter to offer the strategy of Active Surveillance (AS) to patients with locally confined cancers of the prostate. Yet, its interobserver reproducibility is low (48-70%) and its prognostic validity unsatisfactory. An option to complementary assess the malignant potential of these cancers are objective DNA-ploidy-measurements on existing biopsies. For that purpose chromosomal heterogeneity is indirectly quantified by DNA-cytometry resulting in DNA-grades of malignancy 1-4. This review systematically trawls and evaluates all scientific publications on the potential diagnostic and prognostic validity and the heterogeneity of DNA-ploidy measurements in cancers of the prostate between 1966 and 2013. Publications have been classified into Oxford levels of evidence and levels of significance were given for the correlation of DNA-ploidy with different clinical outcomes. 114 scientific articles had to be excluded because of different methodological reasons. All but one of the 67 methodologically acceptable articles report on a significant diagnostic resp. prognostic significance of DNA ploidy measurements in cancers of the prostate. 8 level 1b studies report that DNA-ploidy, assessed on punch biopsies independently predicts organ confinement as assessed after radical prostatectomy. 18 level 2b studies prove that DNA-ploidy measurements add statistically significant information to the Gleason-score. 16 level 2b investigations report a significant correlation of DNA-ploidy with recurrence-free survival. 15 level 2b studies document a significant correlation with overall survival after different types of therapy. 5 level 2b investigations prove a significant correlation with local recurrence or progress after radical prostatectomy. 3 level 2b publications show a significant correlation of DNAploidy with the occurrence of lymph node-or bone metastases after radical prostatectomy. 1 level 2b study documents the additional prognostic value of DNA-ploidy measurements over conventional subjective grading in prostate cancer patients under AS. All existing 15 narrative reviews on selected articles dealing with prognostic DNA-cytometry in cancers of the prostate are in favor of this method. Prospective level 1b studies, especially those proving the validity of DNA ploidy measurements to predict non-progression in patients with clinically insignificant low-grade low stage cancers of the prostate eligible for Active Surveillance additionally to the Gleason-score are still missing.

show abstract

“…Immediately after removal, prostates were inked over the entire surface and fixed overnight in undiluted (37%) formalin. All specimens were processed according to the Stanford technique [9,13,14] with serial sectioning of the specimen at 3-mm intervals in transverse planes perpendicular to the rectal surface. A single coronal cut was prepared from each seminal vesicle.…”

Section: Introductionmentioning

confidence: 99%

“…When tested alone, ploidy had no ability to predict recurrence (p = 0.26). However, in a subset of patients with 4-8 cm3 of cancer with <30% Gleason grade 4 or 5 tumor, ploidy conferred significant additional prognostic information (p < 0.005).ysis of deoxyribonucleic acid (DNA) content of malignant cells by flow cytometry has been proposed as a useful additional prognostic measure which has been the subject of several recent investigations [2][3][4][5][6][7][8][9]. We have shown that tumor volume and Gleason grade are reliable predictors of capsular penetration, seminal vesicle invasion, lymph node metastasis and recurrence [10,11].…”

mentioning

confidence: 99%

Pathologie Parameters and Flow Cytometric Ploidy Analysis in Predicting Recurrence in Carcinoma of the Prostate

Voges¹,

Eigner²,

Ross³

et al. 1993

Eur Urol

View full text Add to dashboard Cite

Value of Nuclear DNA Ploidy Patterns in Patients with Prostate Cancer after Radical Prostatectomy

Cited by 25 publications

References 21 publications

Pre- and Postoperative DNA Ploidy Patterns Correlated to Pt-Stage, Histological Grade and Tumour Volume in Total Prostatectomy Specimens

Pre- and Postoperative DNA Ploidy Patterns Correlated to Pt-Stage, Histological Grade and Tumour Volume in Total Prostatectomy Specimens

DNA-cytometric grading of prostate cancer Systematic review with descriptive data analysis

Pathologie Parameters and Flow Cytometric Ploidy Analysis in Predicting Recurrence in Carcinoma of the Prostate

Contact Info

Product

Resources

About