Value of reflex testing based on hypogammaglobulinemia as demonstrated in serum protein electrophoresis

Chan, Pak Cheung; Chen, Jialun

doi:10.1016/j.clinbiochem.2015.03.014

Cited by 12 publications

(2 citation statements)

References 36 publications

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“…3,8 Reportedly, ~90% of human fLConly or non-secretory multiple myeloma cases have hypogammaglobulinemia. 9,10 Hypogammaglobulinemia was present in our sMRD cases, including the fLC cases but was not limited to sMRD in this case series; γ-globulin suppression was found in cases of T-cell lymphoma and other cases with a polyclonal lymphoid population documented by PARR. Hypoglobulinemia is expected in cases of myelosuppression, drug toxicity, common variable immune deficiency, acquired immune deficiency, and failure of passive transfer, among others.…”

Section: Discussionmentioning

confidence: 52%

Diagnostic performance of routine electrophoresis and immunofixation for the detection of immunoglobulin paraproteins (M‐Proteins) in dogs with multiple myeloma and related disorders: Part 2—Toward improved diagnostic performance

Moore

Harris

Jeffries

et al. 2021

Veterinary Clinical Pathol

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Background:The diagnostic performance of routine electrophoresis (agarose gel electrophoresis [AGE] and capillary zone electrophoresis [CZE]) and species-specific immunofixation (IF) for the detection of immunoglobulin paraproteins (M-proteins) and diagnosis of secretory myeloma-related disorders (sMRD) can be improved. Available canine IF targets were IgG-FC, IgA, IgM, light chain (LC), IgG4, and free LC (fLC) antibodies. Objective: We aimed to review specific features associated with the presence of M-proteins in canine serum samples and the common features causing inaccurate reporting of M-proteins to improve the diagnostic performance of routine electrophoresis and IF for the detection of M-proteins. Methods: Features found in AGE, CZE, routine IF, IgG4 IF, and fLC IF of 100 canine serum samples from Part 1 of this study were evaluated by simple and multivariate logistic regression to identify factors associated with the presence of M-proteins.Cases falsely called negative or positive for M-proteins were reviewed to identify the common features that could be used to increase the diagnostic performance of SPE and IF for M-protein detection. Results:The presence of hypogammaglobulinemia or any peak taller than albumin was associated with an M-protein. Total protein concentrations, globulin concentrations, or peaks wider than albumin were not associated with an M-protein. Free LC sMRD cases were not diagnosed by SPE and routine IF. Cases with infectious and inflammatory etiologies had a restricted polyclonal gammopathy with multiple γglobulin restrictions resulting in some false-positive results. SPE combined with all available IF results and the specific features identified in this study had an estimated sensitivity of 95.1% and specificity of 81.4%. Conclusions:The identified criteria of this study increase the diagnostic performance of the electrophoretic evaluation for M-proteins.

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Section: Discussionmentioning

confidence: 52%

Diagnostic performance of routine electrophoresis and immunofixation for the detection of immunoglobulin paraproteins (M‐Proteins) in dogs with multiple myeloma and related disorders: Part 2—Toward improved diagnostic performance

Moore

Harris

Jeffries

et al. 2021

Veterinary Clinical Pathol

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“…Such a test has the obvious advantage over the existing laboratory tests, i.e. , radio-immuno assay [ 19 ], immunofixation electrophoresis [ 20 ], enzyme-linked immunosorbent assay (ELISA) [ 2 ] and immunoblotting [ 21 ], etc. , in that it can be performed immediately beside the patient giving a result in a few minutes of time instead of several days when the sample is sent for analysis to a laboratory.…”

Section: Discussionmentioning

confidence: 99%

A Novel Point-of-Care Biomarker Recognition Method: Validation by Detecting Marker for Diabetic Nephropathy

et al. 2015

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Biological fluid collection to identify and analyze different disease markers is a routine and normal procedure in health care settings. Body fluids are as varied as urine, blood, mucus, cerebrospinal fluid (CSF), tears, semen, etc. The volumes of the collected fluids range from micro liters (e.g., tears, CSF) to tens and hundreds of milliliters (blood, urine, etc.). In some manifestations, a disease marker (particularly protein markers) can occur in trace amounts, yet the fluids collected are in large volumes. To identify these trace markers, cumbersome methods, expensive instruments, and trained personnel are required. We developed an easy method to rapidly capture, concentrate, and identify protein markers in large volumes of test fluids. This method involves the utilization of two antibodies recognizing two different epitopes of the protein biomarker. Antibody-1 helps to capture and concentrate the biomarker and Antibody-2 adsorbed or conjugated to nanogold beads will detect the biomarker. This method was validated in capturing and detecting lipocalin type prostaglandin-D2 synthase, a marker in urine that implicates diabetic nephropathy. A one-step collection, concentration, and detection device was designed based on this method. This device can replace many of the normal body fluid collection devices such as tubes and containers. A one-step fluid collection and biomarker capture and concentration device for rapid diagnosis of diseases has tremendous advantage in terms of cost and providing timely results.

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Analytical sensitivity and diagnostic performance of serum protein electrophoresis on the HYDRAGEL 30 PROTEIN(E) β1-β2 Sebia Hydrasys system

Tsui

Thomas

Hunt³

et al. 2018

Clinical Biochemistry

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Value of reflex testing based on hypogammaglobulinemia as demonstrated in serum protein electrophoresis

Cited by 12 publications

References 36 publications

Diagnostic performance of routine electrophoresis and immunofixation for the detection of immunoglobulin paraproteins (M‐Proteins) in dogs with multiple myeloma and related disorders: Part 2—Toward improved diagnostic performance

Diagnostic performance of routine electrophoresis and immunofixation for the detection of immunoglobulin paraproteins (M‐Proteins) in dogs with multiple myeloma and related disorders: Part 2—Toward improved diagnostic performance

A Novel Point-of-Care Biomarker Recognition Method: Validation by Detecting Marker for Diabetic Nephropathy

Analytical sensitivity and diagnostic performance of serum protein electrophoresis on the HYDRAGEL 30 PROTEIN(E) β1-β2 Sebia Hydrasys system

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