Value of the Serum Alpha-&lt;i&gt;L&lt;/i&gt;-Fucosidase Activity in the Diagnosis of Colorectal Cancer

Ayude, Daniel; Fernández-Rodrı́guez, Julia; Rodrı́guez-Berrocal, Francisco Javier; Martínez-Zorzano, Vicenta S.; Carlos, Alejandro de; Gil‐Martín, Emilio; Cadena, Marı́a Páez de la

doi:10.1159/000012188

Cited by 55 publications

(49 citation statements)

References 14 publications

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“…We have also found that in preoperative serum from CRC patients AFU activity is significantly decreased compared to healthy controls [12]. A diminished AFU activity is in agreement with the higher levels of L -fucose detected in both tumour and serum of CRC patients [13].…”

Section: Introductionsupporting

confidence: 66%

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Preoperative Serum Alpha-<i>L</i>-Fucosidase Activity as a Prognostic Marker in Colorectal Cancer

Ayude

Cadena²,

Martínez-Zorzano³

et al. 2002

Oncology

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Objectives: The aim of this study was to examine the prognostic value of the preoperative serum α-L-fucosidase (AFU) activity in colorectal cancer and to assess whether it could add prognostic information that Dukes’ stages do not give. Methods: A postoperative follow-up of 137 colorectal cancer patients was performed, and survival analyses were carried out to evaluate the impact of AFU activity on disease-free survival. Dukes’ stage classification, preoperative serum carcinoembryonic antigen levels and six other clinicopathological features of the patients were also analysed. Results: In previous studies, we have stressed the diagnostic value of AFU activity in preoperatively obtained serum from colorectal cancer patients. In the present work, we have found that the enzymatic activity of serum AFU was not related to the Dukes’ stage of the primary tumour, but it was associated with the type of metastasis and recurrence of the disease. The mean value of preoperative serum AFU activity was higher in patients with distant metastases than in those with lymph node or peritoneal metastases, or without metastasis (p = 0.034). After a mean postoperative follow-up period of 22 months, three groups of patients with different recurrence rates could be distinguished (p = 0.0014). Similar results were found when only patients in Dukes’ stage B (p = 0.0439) or C (p = 0.0122) were considered. Conclusions: According to our findings, serum AFU activity appears to be a good prognostic factor of tumour recurrence in colorectal carcinoma. Furthermore, patients in Dukes’ stage B or C at high or very high risk of tumour recurrence could be spotted.

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Section: Introductionsupporting

confidence: 66%

“…Serum AFU activity was measured as previously described [12], using the synthetic substrate 4-methyl-umbelliferyl-α- L -fucopyranoside (4-MU-fucoside; Sigma, St. Louis, Mo., USA). One unit of enzyme was defined as the amount of enzyme required to hydrolyse 1 nmol of substrate per minute at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Preoperative Serum Alpha-<i>L</i>-Fucosidase Activity as a Prognostic Marker in Colorectal Cancer

Ayude

Cadena²,

Martínez-Zorzano³

et al. 2002

Oncology

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“…Carcinoembryonic antigen (CEA) levels in serum samples from 109 colorectal cancer patients and 72 healthy donors were analyzed by specific immunoassay using the Enzymun-Test CEA (Boehringer Mannheim, Germany) as previously described (15). CEA concentrations are expressed in ng/mL serum.…”

Section: Biochemical Assaysmentioning

confidence: 99%

Clinical Significance of Preoperative Serum Sialic Acid Levels in Colorectal Cancer: Utility in the Detection of Patients at High Risk of Tumor Recurrence

et al. 2004

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This study was conducted to evaluate the significance of preoperative serum sialic acid levels in the diagnosis and prognosis of colorectal cancer (CRC). Total sialic acid (TSA) was determined by the thiobarbituric acid method and normalized to total protein (TP). A postoperative follow-up of CRC patients classified as Dukes' stages A, B or C was performed and survival analysis was carried out to evaluate the impact of sialic acid levels on tumor recurrence. Our diagnostic studies indicate that TSA/TP is a better marker than either TSA or carcinoembryonic antigen (CEA), especially for the detection of CRC patients at an early stage. At a cutoff of 30.90 nmol/mg of protein, TSA/TP showed a sensitivity of 85% with a specificity of 97% to discriminate CRC patients from healthy donors. In survival analysis, both TSA and TSA/TP were found to be significant prognostic factors for tumor recurrence in CRC. Furthermore, TSA/TP could distinguish patients at high risk of recurrence within Dukes' stage B and in multivariate analysis it was identified as the best independent prognostic factor. According to our results, preoperative serum TSA/TP content could supply additional information to that provided by Dukes' stage about the prognosis of CRC patients.

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“…The control group, consisting of 52 healthy blood donors (23 females, 29 males) was provided by the Centro de Transfusion de Galicia throughout 1997. Some data of the population samples came from other studies (Fernández-Rodríguez et al, 2000;Ayude et al, 2000).…”

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confidence: 99%

Preoperative serum CD26 levels: diagnostic efficiency and predictive value for colorectal cancer

et al. 2000

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CD26 is an ectoenzyme with dipeptidyl peptidase IV activity expressed on a variety of cell types. Although the function of the high concentration of serum-soluble CD26 (sCD26) is unknown, it may be related to the cleavage of biologically active polypeptides. As CD26 or enzymatic activity levels were previously associated with cancer, we examined the potential diagnostic and prognostic value of preoperative sCD26 measurements by ELISA in colorectal carcinoma patients. We found a highly significant difference between sCD26 levels in healthy donors (mean 559.7 ± 125.5 μg l –1 ) and cancer patients (mean 261.7 ± 138.1 μg l –1 ) ( P < 0.001). A cut-off at 410 μg l –1 gave 90% sensitivity with 90% specificity which means that the diagnostic efficiency of sCD26 is higher than that shown by other markers, particularly in patients at early stages. Moreover, sCD26 as a variable is not related with Dukes’ stage classification, age, gender, tumour location or degree of differentiation. With a follow-up of 2 years until recurrence, preliminary data show that sCD26 can be managed as a prognostic variable of early carcinoma patients. In addition, the origin of sCD26 is discussed. © 2000 Cancer Research Campaign

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Value of the Serum Alpha-<i>L</i>-Fucosidase Activity in the Diagnosis of Colorectal Cancer

Cited by 55 publications

References 14 publications

Preoperative Serum Alpha-<i>L</i>-Fucosidase Activity as a Prognostic Marker in Colorectal Cancer

Preoperative Serum Alpha-<i>L</i>-Fucosidase Activity as a Prognostic Marker in Colorectal Cancer

Clinical Significance of Preoperative Serum Sialic Acid Levels in Colorectal Cancer: Utility in the Detection of Patients at High Risk of Tumor Recurrence

Preoperative serum CD26 levels: diagnostic efficiency and predictive value for colorectal cancer

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