Values of OAS gene family in the expression signature, immune cell infiltration and prognosis of human bladder cancer

Abstract

Background Bladder cancer (BLCA) is one of the most common genitourinary malignancies in the world, but its pathogenic genes have not been fully identified and the treatment outcomes are still unsatisfactory. Although the members of 2', 5'-oligoadenylate synthetase (OAS) gene family are known involved in some tumorous biological processes, the roles of the OAS gene family in BLCA are still undetermined. Methods By combining vast bioinformatic datasets analyses of BLCA and the experimental verification on clinical BLCA specimen, we identified the expressions and biological functions of OAS gene family members in BLCA with comparison to normal bladder tissues. Results The expression levels of OAS gene family members were higher in BLCA than in normal bladder tissues. The expression levels of most OAS genes had correlations with genomic mutation and methylation, and with the infiltration levels of CD4 + T cells, CD8 + T cells, neutrophils, and dendritic cells in the microenvironment of BLCA. In addition, high expressions of OAS1, OAS2, OAS3, and OASL predicted better overall survival in BLCA patients. Conclusions The highly expressed OAS genes in BLCA can reflect immune cells infiltration in the tumor microenvironment and predict the better overall survival of BLCA, and thus may be considered as a signature of BLCA. The study provides new insights into the diagnosis, treatment, and prognosis of BLCA.

Thymic dendritic cell-derived IL-27p28 promotes the establishment of functional bias against IFN-γ production in newly generated CD4+T cells through STAT1-related epigenetic mechanisms

“…Apart from their antiviral activity, OAS proteins are also involved in cell growth, differentiation, and apoptosis ( Castelli et al, 1997; Huang et al, 2022; Salzberg et al, 1997 ). The genomic mutation and methylation of most OAS genes have been shown to alter their expression levels, which is associated with the levels of infiltrated CD4 + T cells and CD8 + T cells in the tumor microenvironment ( Gao et al, 2022 ). Secreted OAS2 has also been observed to inhibit CD3 zeta chain expression in T cells ( Dar et al, 2016 ).…”

BLCA prognostic model creation and validation based on immune gene-metabolic gene combination

“…The level of OAS1 gene expression is closely linked to tumor onset and progression [ 68 ]. According to research, OAS1 is significantly downregulated in BLCA tissues, and this downregulation is linked to the clinical characteristics and prognosis of the tumor [ 69 , 70 ]. OAS1 enhances the immune cell’s capacity to fight viral infections by triggering the interferon signaling pathway.…”

Peptidase inhibitor 16 promotes proliferation of pancreatic ductal adenocarcinoma cells through OASL signaling

“…As reported, OASL is closely related to viral infection response and autoimmune diseases 13 . In addition, OASL plays a role in a variety of tumors, such as breast cancer, lung cancer, bladder cancer, and gastric cancer 14–17 . It has been reported that OASL is overexpressed in PDAC and is associated with poor outcomes in patients 18 .…”

“…13 In addition, OASL plays a role in a variety of tumors, such as breast cancer, lung cancer, bladder cancer, and gastric cancer. [14][15][16][17] It has been reported that OASL is overexpressed in PDAC and is associated with poor outcomes in patients. 18 Furthermore, we found that Pi16 can promote PDAC cell proliferation by regulating OASL, suggesting that the Pi16/OASL axis may be a promising new target for PDAC diagnosis and treatment.…”