VAMP/synaptobrevin as an acrosomal marker for human sperm

Ramalho‐Santos, João; Schatten, Gerald

doi:10.1016/s0015-0282(01)02957-0

Cited by 7 publications

(3 citation statements)

References 4 publications

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“…Our previous study confirmed that monoclonal antibodies against intra-acrosomal sperm proteins are valuable and sensitive markers for the evaluation of sperm quality [15-20]. A correlation between sperm quality and fertility has been demonstrated using animal and human pathological spermatozoa [18-22] and also by our own results (prepared for publication). We can conclude that although spermatogenesis was established in the P generation, NP had an effect on sperm quality.…”

Section: Discussionsupporting

confidence: 83%

Effects of p-nonylphenol and resveratrol on body and organ weight and in vivo fertility of outbred CD-1 mice

Kyselová

Pěknicová

Buckiová

et al. 2003

Reprod Biol Endocrinol

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The aim of this study was to analyse the multigenerational effects of para-nonylphenol (NP) and resveratrol (RES) on the body weight, organ weight and reproductive fitness of outbred CD-1 mice. The data indicate that in male mice, NP had an effect on the weight of selected reproductive organs and the kidneys in the parental (P) generation males. Effects on selected reproductive organs, the liver and kidneys in the F1-generation males were also seen. In females, effects of NP on body weight and kidney weight were seen in the P generation, but no effects on any measured parameter were seen in the F1 generation. RES had no effect on body weight but did have some effect on selected male and female reproductive organs in the P generation. RES altered the spleen and liver weights of P-generation males and the kidney weight of F1-generation males. Acrosomal integrity (using a monoclonal antibody against intra-acrosomal sperm proteins) was assessed for both generations of NP- and RES-treated mice. A significant reduction in acrosomal integrity was seen in both generations of NP-treated, but not in RES-treated, mice. Fewer offspring were observed in the second litter of the F2 generation of mice treated with NP; no similar effect was seen in RES-treated mice. The litter sex ratio was not different from controls. Unlike RES, NP had a negative effect on spermatogenesis and sperm quality with a resultant impact on in vivo fertility.

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Section: Discussionsupporting

confidence: 83%

Effects of p-nonylphenol and resveratrol on body and organ weight and in vivo fertility of outbred CD-1 mice

Kyselová

Pěknicová

Buckiová

et al. 2003

Reprod Biol Endocrinol

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“…Globozoopermia (round-head spermatozoa) is a familial disorder characterized by the production of abnormally round heads and non-fusiform motile spermatozoa (Schill 1991;Wolff et al 1976). Round-head spermatozoa are incapable of fertilizing oocytes, and in extreme cases, the acrosome is absent (Aitken et al 1990;Ramalho-Santos et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Differential localization of α’ and β subunits of protein kinase CK2 during rat spermatogenesis

et al. 2009

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Protein kinase CK2 is a serine/threonine kinase expressed in organisms from yeast to human and is composed of a catalytic subunit (alpha or alpha') and a regulatory subunit (beta) forming a holoenzyme with the possible subunit combinations alpha(2)beta(2), alpha'(2)beta(2), or alphaalpha'beta(2). This kinase has been shown to be involved in embryonic development and gametogenesis. We have studied the expression of the CK2alpha' and CK2beta subunits during the first wave of spermatogenesis and in adult testis in the rat. Western blot analyses have demonstrated that both CK2alpha' and CK2beta are expressed in testes from birth to adulthood. A more detailed study of the protein localization of CK2alpha' and CK2beta by immunohistochemistry suggests that CK2alpha' and CK2beta are localized in the nuclei of Sertoli cells in 5-day-old rats, whereas they appear to have a cytoplasmic localization in older animals. In adult testes, CK2alpha' and CK2beta subunits are present in spermatocytes. Both subunits exhibit a similar expression pattern with the highest level in spermatocytes at stages VIII-XIV. Interestingly, CK2beta is highly expressed in spermatogonia, whereas CK2alpha' is barely detectable. Mature epididymal spermatozoa express CK2alpha' in the acrosome and CK2beta in the flagellum. This new evidence therefore indicates that protein kinase CK2 has a possible role at various stages during mammalian spermatogenesis, a process that involves proliferation, meiosis, apoptosis, and differentiation. CK2 might thus emerge as a new pivotal control enzyme at various levels in mammalian spermatogenesis.

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“…However, all of these studies show that proacrosomal vesicles form and fuse to give rise to the acrosomal vesicle whether or not it is attached to the nucleus (Anton Lamprecht et al, 1976; Baccetti et al, 1977; Dam, Koscinski, et al, 2007; Perrin et al, 2013; Schirren, 1971). This may explain why round‐headed spermatozoa in the ejaculate may contain a few acrosomal vesicles around the nucleus (Dam, Koscinski, et al, 2007; Ramalho‐Santos et al, 2002; Singh, 1992).…”

Section: The Round‐headed Spermatozoamentioning

confidence: 99%

Human globozoospermia‐related genes and their role in acrosome biogenesis

Moreno

2022

WIREs Mechanisms of Disease

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The mammalian acrosome is a secretory vesicle attached to the sperm nucleus whose fusion with the overlying plasma membrane is required to achieve fertilization. Acrosome biogenesis starts during meiosis, but it lasts through the entire process of haploid cell differentiation (spermiogenesis). Acrosome biogenesis is a stepwise process that involves membrane traffic from the Golgi apparatus, but it also seems that the lysosome/endosome system participates in this process. Defective sperm head morphology is accompanied by defective acrosome shape and function, and patients with these characteristics are infertile or subfertile. The most extreme case of acrosome biogenesis failure is globozoospermia syndrome, which is primarily characterized by the presence of round‐headed spermatozoa without acrosomes with cytoskeleton defects around the nucleus and infertility. Several genes participating in acrosome biogenesis have been uncovered using genetic deletions in mice, but only a few of them have been found to be deleted or modified in patients with globozoospermia. Understanding acrosome biogenesis is crucial to uncovering the molecular basis of male infertility and developing new diagnostic tools and assisted reproductive technologies that may help infertile patients through more effective treatment techniques. This article is categorized under: Reproductive System Diseases > Environmental Factors Infectious Diseases > Stem Cells and Development Reproductive System Diseases > Molecular and Cellular Physiology

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VAMP/synaptobrevin as an acrosomal marker for human sperm

Cited by 7 publications

References 4 publications

Effects of p-nonylphenol and resveratrol on body and organ weight and in vivo fertility of outbred CD-1 mice

Effects of p-nonylphenol and resveratrol on body and organ weight and in vivo fertility of outbred CD-1 mice

Differential localization of α’ and β subunits of protein kinase CK2 during rat spermatogenesis

Human globozoospermia‐related genes and their role in acrosome biogenesis

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