2016
DOI: 10.1038/ncomms10489
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VAMP7 regulates constitutive membrane incorporation of the cold-activated channel TRPM8

Abstract: The cation channel TRPM8 plays a central role in the somatosensory system, as a key sensor of innocuously cold temperatures and cooling agents. Although increased functional expression of TRPM8 has been implicated in various forms of pathological cold hypersensitivity, little is known about the cellular and molecular mechanisms that determine TRPM8 abundance at the plasma membrane. Here we demonstrate constitutive transport of TRPM8 towards the plasma membrane in atypical, non-acidic transport vesicles that co… Show more

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Cited by 44 publications
(48 citation statements)
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References 69 publications
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“…Two main v-SNAREs were shown to mediate distinct exocytic mechanisms: clostridial neurotoxin-sensitive VAMP2 (and the closely related VAMP1 and VAMP3) mediates synaptic vesicle and early endosomal exocytosis, whereas clostridial neurotoxin-insensitive VAMP7 mediates Golgi-derived, late endosomal and lysosomal secretory pathways ( Proux-Gillardeaux et al., 2005a ), which are best defined by the presence of the tetraspanin CD63 ( Chiaruttini et al., 2016 , Coco et al., 1999 ). In the recent years, lysosomal exocytosis has appeared as a very general mechanism that can be found in virtually any cell type ( Ghosh et al., 2016 , Jaiswal et al., 2002 , Li et al., 2008 , Verderio et al., 2012 ). Interestingly enough, VAMP2 and VAMP3 were shown to mediate integrin recycling ( Hasan and Hu, 2010 , Proux-Gillardeaux et al., 2005b , Skalski and Coppolino, 2005 , Tayeb et al., 2005 ), and VAMP7, to play an essential role in cell migration and invasion ( Proux-Gillardeaux et al., 2007 , Steffen et al., 2008 , Williams and Coppolino, 2011 ).…”
Section: Introductionmentioning
confidence: 99%
“…Two main v-SNAREs were shown to mediate distinct exocytic mechanisms: clostridial neurotoxin-sensitive VAMP2 (and the closely related VAMP1 and VAMP3) mediates synaptic vesicle and early endosomal exocytosis, whereas clostridial neurotoxin-insensitive VAMP7 mediates Golgi-derived, late endosomal and lysosomal secretory pathways ( Proux-Gillardeaux et al., 2005a ), which are best defined by the presence of the tetraspanin CD63 ( Chiaruttini et al., 2016 , Coco et al., 1999 ). In the recent years, lysosomal exocytosis has appeared as a very general mechanism that can be found in virtually any cell type ( Ghosh et al., 2016 , Jaiswal et al., 2002 , Li et al., 2008 , Verderio et al., 2012 ). Interestingly enough, VAMP2 and VAMP3 were shown to mediate integrin recycling ( Hasan and Hu, 2010 , Proux-Gillardeaux et al., 2005b , Skalski and Coppolino, 2005 , Tayeb et al., 2005 ), and VAMP7, to play an essential role in cell migration and invasion ( Proux-Gillardeaux et al., 2007 , Steffen et al., 2008 , Williams and Coppolino, 2011 ).…”
Section: Introductionmentioning
confidence: 99%
“…et al, 2000); (3) Golgi homeostasis and Golgi-to-plasma membrane transport (Burgo et al, 2012;Danglot et al, 2010;Ghosh et al, 2016;Molino et al, 2015); (4) selective apical exocytosis and polarity (Galli et al, 1998;Lafont et al, 1999;Pocard et al, 2007;Vogel et al, 2015); (5) it has been implicated in ciliogenesis (Szalinski et al, 2014); and (6) co-fractionates with RTCs on sucrose density gradients (Wang et al, 2012).…”
Section: Introductionmentioning
confidence: 99%
“…Moreover, using a pH-sensitive GFP (pHluorin), 6 we were able to demonstrate that TRPM8-and VAMP7-containing vesicles fuse with the plasma membrane. These vesicles do not undergo a classical fuse-and-collapse type of fusion 7 ; instead, they are part of a vesicle-pool constantly interacting with the PM, in either a very transient or more persistent manner.…”
Section: Moreover Tir-frapmentioning
confidence: 93%
“…6 In the first part of the work, we used Total-Internal-Reflection-Fluorescence-microscopy (TIRFM) to observe near membrane events in live cells. 7 Using this technique, we showed that fluorescently tagged TRPM8 resides in very dynamic vesicles (speed 0.58 mm/s), which exhibit constitutive movement, mainly via microtubules.…”
mentioning
confidence: 99%