Vancomycin architecture dependence on the capture efficiency of antibody-modified microbeads by magnetic nanoparticles

Kell, Arnold J.; Simard, Benoît

doi:10.1039/b617427b

Cited by 20 publications

(26 citation statements)

References 22 publications

(19 reference statements)

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“…With our covalent approach, 6 all carbohydrates are uniformly oriented on the NP surface, which is crucial for high performances in cell-capturing studies. 7 All MGNPs were characterized by X-ray diffraction, transmission electron microscopy (TEM), thermogravimetric analyses, and IR spectroscopy ( Figures S1-4).…”

mentioning

confidence: 99%

Magnetic Glyco-nanoparticles: A Unique Tool for Rapid Pathogen Detection, Decontamination, and Strain Differentiation

2007

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The appealing mechanisms by which nanocomposites knit biomolecules not only lend credence in designing novel nanosensors but considerably advance medical applications. 1 Recent headline news about Escherichia coli (E. coli) contamination in produce and Bacillus anthracis attacks pinpoint the urgent need for an effective method for microbial decontamination and rapid detection without time-consuming cell culturing. It is known that many bacteria use mammalian cell surface carbohydrates as anchors for attachments, which subsequently results in infection. 2 The unique combination of magnetic nanocomposites and diverse carbohydrate bioactivities prompts us to embark on a biosensing research program. Herein, we report a magnetic glyco-nanoparticle (MGNP)-based system to not only detect E. coli within 5 min, but also remove up to 88% of the target bacteria from the medium. Furthermore, the identities of three different E. coli strains were easily determined on the basis of the response patterns to two MGNPs highlighting their potential in biosensing.It is advantageous to use magnetic nanoparticles (NPs) for detection. The high surface/volume ratio offers more contact surface area for attaching carbohydrates and for capturing pathogens. 3 The sizes of NPs are typically about 2 orders of magnitude smaller than a bacterium, which allows the attachment of multiple NPs onto a bacterial cell rendering easy magnet-mediated separation. 3,4 Moreover, the small NPs have faster kinetics in solution as compared to their micrometer-sized counterparts, which can result in fast detection.Our journey commenced with functionalization of silica-coated magnetite NP (NP 1) with D-mannose (Man) through either a triazole linker (MGNP 2) formed by the [2+3] Huisgen reaction 5 or an amide linkage (MGNP 3) (Schemes 1 and S1). With our covalent approach, 6 all carbohydrates are uniformly oriented on the NP surface, which is crucial for high performances in cell-capturing studies. 7 All MGNPs were characterized by X-ray diffraction, transmission electron microscopy (TEM), thermogravimetric analyses, and IR spectroscopy ( Figures S1-4).To ensure that carbohydrates on MGNP retain their binding abilities, the interaction between various MGNPs with a mannose binding lectin, concanavalin A (Con A), 8 was first investigated. Carbohydrate-lectin interaction 9 is central in devising our biosensor. After mixing NPs with fluorescein-labeled Con A, a magnetic field was applied to the mixture through a handheld magnet inducing aggregation of magnetic NPs on the side of the vial. The residual fluorescence of supernatants was then recorded ( Figure S5). With MGNP 3, the emission intensity of the supernatant decreased 87% indicating that most Con A was removed by 3. Triazole linked MGNP 2 was less efficient accounting for a 60% emission decrease probably because of the low efficiency of the Huisgen reaction with immobilized alkynes. 5 NP 1 without carbohydrates (control) did not remove any Con A, proving that the separation of Con A is due to its interaction...

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confidence: 99%

Magnetic Glyco-nanoparticles: A Unique Tool for Rapid Pathogen Detection, Decontamination, and Strain Differentiation

2007

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“…A final 1% solution of succinic anhydride was used. 17 This solution was allowed to react overnight and was rinsed twice in THF the next day to remove any unreacted succinic anhydride. These carboxylate coated SPION ( Figure 1C) were stored under refrigeration in a THF solution where no observable oxidation was seen in a period of less than a month.…”

Section: Carboxylate Functionalized Spionmentioning

confidence: 99%

“…Recently, many studies have also demonstrated the use of magnetic particles coated with antibiotics as an alternative method for drug delivery, albeit such approaches still rely on the use of antibiotics for which bacteria are increasingly developing resistance towards. 12,17 In this study, superparamagnetic iron oxide nanoparticles (SPION) were functionalized with amine, carboxylic acid, or isocyanate groups and, for the first time, were tested for their ability to penetrate S. aureus biofilms to explore a novel non-antibiotic strategy to decrease bacteria growth after a biofilm is formed on a medical device.…”

Section: Introductionmentioning

confidence: 99%

“…[3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] In particular, due to their magnetic properties and location in the body which can be controlled externally through an applied electric field, superparamagnetic iron oxide nanoparticles (SPION) have a strong potential to disrupt biofilms produced by MRSA. [4][5][6]19 These iron oxide nanoparticles (which can be in the form of maghemite, γ-Fe 2 O 3, or magnetite, Fe 3 O 4 ) are materials that can control drug delivery via an external magnetic source, the ability of which can be further improved by functionalizing their surface with molecules or chemistries to better penetrate biofilms and bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Short communication: carboxylate functionalized superparamagnetic iron oxide nanoparticles (SPION) for the reduction of S. aureus growth post biofilm formation

Leuba¹,

Durmus²,

Taylor³

et al. 2013

IJN

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“…Recent literature has reported the use of vancomycin-functionalized magnetic nanoparticles as a noncovalent probe for capturing gram-positive bacteria. 29 Vancomycin is a pentapeptide glycoprotein antibiotic natural product that targets the D-alanine-D-alanine motif in gram-positive PG.…”

mentioning

confidence: 99%

Bioorthogonal labelling of living bacteria using unnatural amino acids containing nitrones and a nitrone derivative of vancomycin

et al. 2015

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Unnatural D-amino acids bearing endocyclic nitrones were developed for live-cell labelling of the bacterial peptidoglycan layer. Metabolic incorporation of D-Lys and D-Ala derivatives bearing different endocyclic nitrones was observed in E. coli, L. innocua, and L. lactis. The incorporated nitrones of these bacteria then rapidly underwent strain-promoted alkyne-nitrone cycloaddition (SPANC) reactions affording chemically modified bacteria.

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Vancomycin architecture dependence on the capture efficiency of antibody-modified microbeads by magnetic nanoparticles

Abstract: We show that the ability to control the architecture/orientation of vancomycin on the surface of magnetic nanoparticles has a drastic effect on the ability of the nanoparticles to magnetically confine vancomycin-antibody modified polystyrene microbeads.

Cited by 20 publications

References 22 publications

Magnetic Glyco-nanoparticles: A Unique Tool for Rapid Pathogen Detection, Decontamination, and Strain Differentiation

Magnetic Glyco-nanoparticles: A Unique Tool for Rapid Pathogen Detection, Decontamination, and Strain Differentiation

Short communication: carboxylate functionalized superparamagnetic iron oxide nanoparticles (SPION) for the reduction of S. aureus growth post biofilm formation

Bioorthogonal labelling of living bacteria using unnatural amino acids containing nitrones and a nitrone derivative of vancomycin

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