Vancomycin-loaded silk fibroin microspheres in an injectable hydrogel for chronic osteomyelitis therapy

Zeng

J of Applied Polymer Sci

et al. 2023

The flexible wearable hydrogel strain sensors for human motion monitoring have attracted broad attention. However, developing a high‐strength hydrogel strain sensor remains challenging and achieves synergistic characteristics such as biocompatibility, antibacterial activity, and conductivity. This work proposes a two‐step UV polymerization method for the preparation of (hydrolyzed silk fibroin (HSF)/polyacrylamide (PAM)/polyacrylic acid (PAA)/poly sulfobetaine methacrylate (PSBMA) composite hydrogels. The monomers AM and AA undergo self‐polymerization and copolymerization. Meanwhile, SF is cross‐linked with them via multiple hydrogen bonds to form the first network. After the second UV initiation, SBMA polymerizes to create the second network. The HSF8%/PAM/PAA/PSBMA2% possesses superior tensile strength (1.49 ± 0.07 MPa) and compressive modulus (21.47 ± 1.07 MPa at 99% strain). The SBMA (4 wt%) endows hydrogels with antibacterial activity (98.53% ± 4.93%). The H+ ionized by PAA and the ion channels constructed by SBMA (4 wt%) provide hydrogel with ionic conductivity (0.950 S m−1). The hydrogel could be used as a flexible strain sensor to monitor human joint activity. The natural silk fibroin allows the hydrogel to retain degradability. Therefore, the SF‐based conductive hydrogel shows the potential application in flexible strain sensors.

Section: Introductionmentioning

confidence: 99%

Silk fibroin‐based conductive and antibacterial hydrogels with a dual network for flexible sensors

Zeng

J of Applied Polymer Sci

et al. 2023

Front. Bioeng. Biotechnol.

“…At present, a large number of antibiotic sustained-release materials have been developed to treat osteomyelitis ( Hasan et al, 2019 ; Xu et al, 2021 ; Feng et al, 2022 ; Gritsch et al, 2022 ; Wu et al, 2022 ), but the treatment effect is still not ideal and the recurrence rate is high. Zhou ( Zhang et al, 2023 ) et al reported Vancomycin-loaded silk fibroin microspheres in an injectable hydrogel for chronic osteomyelitis therapy, demonstrating a sustained-release profile and good biocompatibility, making it promising for application in osteomyelitis treatment. Ju ( Ju et al, 2023 ) reported a novel Zn 2+ incorporated composite polysaccharide microspheres for sustained growth factor release and wound healing.…”

Section: Introductionmentioning

confidence: 99%

Preparation of fish collagen and vancomycin microspheres based on microfluidic technology and its application in osteomyelitis

Hu,

Tang,

et al. 2023

At present, the clinical treatment of osteomyelitis and osteomyelitis-induced bone defects is challenging, easy to recur, drug toxic side effects, secondary or multiple surgeries, etc. The design of biodegradable composite biomaterials to improve antibiotics in the local precise anti-infection at the same time to complete the repair of bone defects is the current research hot spot. Herein, a composite hydrogel with a double bond at the end (FA-MA) was prepared by affinity addition reaction between fish collagen (FA) and methacrylic anhydride (MA) under photoinitiator initiation conditions, then, FA-MA was amino-activated by EDC/NHC, and vancomycin was attached to FA-MA via amide bonding to prepare FA-MA-Van hydrogels, and finally, the composite hydrogel microspheres were prepared by microfluidic technology. The structure of the hydrogel was confirmed by SEM (elemental analysis), optical microscopy, FTIR, and XPS to confirm the successful preparation. The composite hydrogel microspheres showed the better antimicrobial effect of hydrogel microspheres by bacterial coated plate experiments and SEM morphology results, with the antimicrobial class reaching 99.8%. The results of immunofluorescence staining and X-ray experiments showed that the hydrogel microspheres had a better effect on promoting bone repair. This engineered design of hydrogel microspheres provides clinical significance for treating osteomyelitis at a later stage.

Multifunctional Hydrogel Based on Silk Fibroin Promotes Tissue Repair and Regeneration

Lin,

Li,

Wang

et al. 2024

Adv Funct Materials

The creation of functional hydrogels with robust load‐bearing capacity adaptable to complex tissue regeneration remains challenging. Silk fibroin (SF) is a natural biomaterial with excellent mechanical strength and cell adhesion capacity, possessing tremendous potential to solve the aforementioned dilemma. The excellent biodegradability and biocompatibility and the molecular structure with multiple modifiable moieties provide opportunities for the injectability and multifunctionality of hydrogels. Furthermore, the incorporation of other polymers or active ingredients can improve the basic properties, confer biological activities and pharmacological effects, and provide the prerequisites for hydrogels to fulfill specific requirements. Therefore, SF‐based hydrogels are widely applied in tissue repair and regeneration, especially in bone, skin, nerve, liver, myocardium, and cornea, which have effectively addressed the challenges of scaffolds' lack of cell adhesion sites, excessive degradation, as well as the lack of efficient, long‐lasting antimicrobial properties. This review outlines the properties of SF, discusses the types of materials commonly used in the preparation of hydrogels and their characteristics, and describes the current construction methods of SF hydrogels. Simultaneously, recent advancements in applying SF‐based multifunctional hydrogels within biological tissues are explored, with a focus on their role in tissue repair, highlighting the repair mechanism of SF based on bone and skin.