Vancomycin-soaked wrapping of harvested hamstring tendons during anterior cruciate ligament reconstruction. A review of the ‘vancomycin wrap’

Jefferies, James G.; Aithie, Joanna M S; Spencer, S.J.

doi:10.1016/j.knee.2019.04.008

Cited by 22 publications

(26 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The first description of the ''vancomcyin wrap'' for ACLR was in 2012; since then, a number of studies have demonstrated its effectiveness in combination with usual IV antibiotic prophylaxis at reducing postoperative infection rates to almost 0%. 5,14,19,[25][26][27]34 One study reported infection rates in the control group, treated with prophylactic IV antibiotics alone, as 1.4% (n = 285) compared with 0% in patients who were treated with prophylactic IV antibiotics in combination with vancomycin wrap (n = 870). 34 A further study that conducted a postoperative follow-up of 1300 consecutive ACLRs using the vancomycin wrap in combination with standard IV antibiotic prophylaxis reported zero postoperative infections against a retrospective control group (n = 240) that reported infections in 2.4% of patients.…”

Section: Discussionmentioning

confidence: 99%

“…34 Since then, there have been a number of published studies and reviews that have demonstrated significantly reduced postoperative infection rates in ACLR. 5,14,19,[25][26][27]34 The molecular effects of vancomycin on human and animal periarticular tissues, including tendon, has a limited evidence base. Porcine chondrocyte death was significantly increased after exposure to vancomycin at doses of 5 mg/mL or higher.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Vancomycin Wrap for Anterior Cruciate Ligament Surgery: Molecular Insights

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background: The use of the vancomycin wrap to pretreat the hamstring graft in anterior cruciate ligament reconstruction (ACLR) has grown in popularity since it was first described in 2012 and has significantly reduced rates of postoperative infection. However, it remains unknown if this antibiotic treatment affects the molecular composition of the graft. Purpose: To establish whether treatment with vancomycin at 5 mg/mL, the most commonly used concentration, alters the molecular function of the hamstring graft in ACLR. Study Design: Controlled laboratory study. Methods: Surplus hamstring tendon collected after routine ACLR surgery was used for in vitro cell culture and ex vivo tissue experiments. Vancomycin was used at 5 mg/mL in RPMI or saline diluent to treat cells and tendon tissue, respectively, with diluent control conditions. Cell viability at 30, 60, and 120 minutes was assessed via colorimetric viability assay. Tendon cells treated with control and experimental conditions for 1 hour was evaluated using semiquantitative reverse transcription analysis, immunohistochemistry staining, and protein quantitation via enzyme-linked immunosorbent assay for changes in apoptotic, matrix, and inflammatory gene and protein expression. Results: Vancomycin treatment at 5 mg/mL significantly reduced tenocyte viability in vitro after 60 minutes of treatment ( P < .05); however, this was not sustained at 120 minutes. Vancomycin-treated tendon tissue showed no significant increase in apoptotic gene expression, or apoptotic protein levels in tissue or supernatant, ex vivo. Vancomycin was associated with a reduction in inflammatory proteins from treated tendon supernatants (IL-6; P < .05). Conclusion: Vancomycin did not significantly alter the molecular structure of the hamstring graft. Reductions in matrix protein and inflammatory cytokine release point to a potential beneficial effect of vancomycin in generating a homeostatic environment. Clinical Relevance: Vancomycin ACL wrap does not alter the molecular structure of the ACL hamstring graft and may improve graft integrity.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Vancomycin Wrap for Anterior Cruciate Ligament Surgery: Molecular Insights

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…After the treatment failure of penicillins and semisynthetic penicillins, vancomycin has been the agent of choice to treat methicillin-resistant Staphylococcus aureus (MRSA) infections [9]. Unfortunately, multiresistance and toxicity of this antibiotic cannot be negligible [10, 11] and make the management of all those bacterial infections increasingly difficult which is a major prevalent worldwide cause of healthcare [12] and a significant cause of global morbidity and mortality [13]. This situation entails intensified efforts to develop alternative treatment.…”

Section: Introductionmentioning

confidence: 99%

Antistaphylococcal Activity and Phytochemical Analysis of Crude Extracts of Five Medicinal Plants Used in the Center of Morocco against Dermatitis

Zeouk

Balouiri

Bekhti

2019

International Journal of Microbiology

View full text Add to dashboard Cite

Novel drugs for methicillin-resistant Staphylococcus aureus (MRSA) hospital- and community-acquired infections are needed because of the emergence of resistance against antibiotics. In this study, methanolic and aqueous extracts of Berberis hispanica, Crataegus oxyacantha, Cistus salviifolius, Ephedra altissima, and Lavandula dentata selected from an ethnopharmacological study to treat skin infections in Sefrou city (Center of Morocco) were tested for their antistaphylococcal activity against strains often involved in cutaneous disorders: two methicillin-resistant Staphylococcus aureus strains and one strain of Staphylococcus epidermidis using the well-diffusion assay, while the agar macrodilution method was used to determine the minimal inhibitory concentrations. The total phenolic compounds and flavonoid contents of all tested extracts were also evaluated. Three of the five methanolic extracts showed an important antibacterial activity. Berberis hispanica extract was the most active with a minimal inhibitory concentration of 04.00 mg/ml against all tested strains, followed by Cistus salviifolius and Crataegus oxyacantha extracts containing the highest amounts of total phenols (133.83 ± 9.03 and 140.67 ± 3.17 μg equivalent of gallic acid/mg of extract). However, the aqueous extracts have not shown any activity against the tested strains. The current data suggested that the most active extracts can be a good source of natural antistaphylococcal compounds and warrants further investigations to isolate bioactive molecules.

show abstract

“…This systematic review included level three and four studies as well as abstracts and is therefore not reliable. The second systematic review was more skeptical of the level of evidence regarding the use of vancomycin and concluded that more research is needed [10].…”

mentioning

confidence: 99%

Nationwide survey on the use of local antibiotics during anterior cruciate ligament reconstruction in Norway

Sailer

Skråmm

Sivertsen

et al. 2020

Preprint

View full text Add to dashboard Cite

Objective: The use of local antibiotics in surgical reconstruction of the anterior cruciate ligament (ACLR) in Europe and Scandinavia is increasing.We wanted to survey the scope of this practice in Norway. In cooperation with the Norwegian cruciate ligament registry we contacted all institutions in Norway performing ACLR to find out.Results: The results showed that the practice of local antibiotics in ACLR is not widely used in Norway.

show abstract

Vancomycin-soaked wrapping of harvested hamstring tendons during anterior cruciate ligament reconstruction. A review of the ‘vancomycin wrap’

Cited by 22 publications

References 42 publications

Vancomycin Wrap for Anterior Cruciate Ligament Surgery: Molecular Insights

Vancomycin Wrap for Anterior Cruciate Ligament Surgery: Molecular Insights

Antistaphylococcal Activity and Phytochemical Analysis of Crude Extracts of Five Medicinal Plants Used in the Center of Morocco against Dermatitis

Nationwide survey on the use of local antibiotics during anterior cruciate ligament reconstruction in Norway

Contact Info

Product

Resources

About