Vanillin Inhibits Translation and Induces Messenger Ribonucleoprotein (mRNP) Granule Formation in Saccharomyces cerevisiae: Application and Validation of High-Content, Image-Based Profiling

Iwaki, Asako; Ohnuki, Shinsuke; Suga, Y.; Izawa, Shingo; Ohya, Yoshikazu

doi:10.1371/journal.pone.0061748

Cited by 80 publications

(69 citation statements)

References 39 publications

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“…3D). Because mutants displaying a high correlation with a drug phenotype can help identify targeted processes, we next compared the morphology of poacic acid-treated cells with the individual morphologies of 4,718 yeast deletion mutants (37,39,40). Forty-three deletion mutants had morphological profiles statistically similar (P < 0.01) to those of poacic acid ( Fig.…”

Section: Morphological Analysis Revealed That Poacic Acid Affects Thementioning

confidence: 99%

Plant-derived antifungal agent poacic acid targets β-1,3-glucan

Piotrowski

Okada

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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136

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A rise in resistance to current antifungals necessitates strategies to identify alternative sources of effective fungicides. We report the discovery of poacic acid, a potent antifungal compound found in lignocellulosic hydrolysates of grasses. Chemical genomics using Saccharomyces cerevisiae showed that loss of cell wall synthesis and maintenance genes conferred increased sensitivity to poacic acid. Morphological analysis revealed that cells treated with poacic acid behaved similarly to cells treated with other cell wall-targeting drugs and mutants with deletions in genes involved in processes related to cell wall biogenesis. Poacic acid causes rapid cell lysis and is synergistic with caspofungin and fluconazole. The cellular target was identified; poacic acid localized to the cell wall and inhibited β-1,3-glucan synthesis in vivo and in vitro, apparently by directly binding β-1,3-glucan. Through its activity on the glucan layer, poacic acid inhibits growth of the fungi Sclerotinia sclerotiorum and Alternaria solani as well as the oomycete Phytophthora sojae. A single application of poacic acid to leaves infected with the broad-range fungal pathogen S. sclerotiorum substantially reduced lesion development. The discovery of poacic acid as a natural antifungal agent targeting β-1,3-glucan highlights the potential side use of products generated in the processing of renewable biomass toward biofuels as a source of valuable bioactive compounds and further clarifies the nature and mechanism of fermentation inhibitors found in lignocellulosic hydrolysates.

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Section: Morphological Analysis Revealed That Poacic Acid Affects Thementioning

confidence: 99%

Plant-derived antifungal agent poacic acid targets β-1,3-glucan

Piotrowski

Okada

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

136

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“…In the same study, S. cerevisiae was also used, but an additional activation enzyme was needed. Vanillin is moderately toxic to yeast cells (it represses translational processes [31]). It was shown that adding a glycosyl moiety, by expressing a 1-UDP-glycosyltransferase, leads to the conversion of vanillin to vanillin-glucoside (VG), which markedly increased production levels.…”

Section: Phenylpropanoidsmentioning

confidence: 99%

The Smell of Synthetic Biology: Engineering Strategies for Aroma Compound Production in Yeast

2018

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Yeast-especially Saccharomyces cerevisiae-have long been a preferred workhorse for the production of numerous recombinant proteins and other metabolites. S. cerevisiae is a noteworthy aroma compound producer and has also been exploited to produce foreign bioflavour compounds. In the past few years, important strides have been made in unlocking the key elements in the biochemical pathways involved in the production of many aroma compounds. The expression of these biochemical pathways in yeast often involves the manipulation of the host strain to direct the flux towards certain precursors needed for the production of the given aroma compound. This review highlights recent advances in the bioengineering of yeast-including S. cerevisiae-to produce aroma compounds and bioflavours. To capitalise on recent advances in synthetic yeast genomics, this review presents yeast as a significant producer of bioflavours in a fresh context and proposes new directions for combining engineering and biology principles to improve the yield of targeted aroma compounds.

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“…In the same study, S. cerevisiae was also used, but an additional activation enzyme was needed. Vanillin is moderately toxic to yeast cells (it represses translational processes [30]) . It was shown that adding a glycosyl moiety, by expressing a 1-UDPglycosyltransferase, leads to the conversion of vanillin to vanillin-glucoside (VG) which markedly increased production levels.…”

Section: Phenylpropanoidsmentioning

confidence: 99%

The Smell of Synthetic Biology: Engineering Strategies for Aroma Compound Production in Yeast

Wyk¹,

Kroukamp²,

Pretorius³

2018

Preprint

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Yeast -especially Saccharomyces cerevisiae -have long been a preferred workhorse for the production of numerous recombinant proteins and other metabolites. S. cerevisiae is a noteworthy aroma compound producer, and has also been exploited to produce foreign bioflavour compounds. In the past few years, important strides have been made in unlocking the key elements in the biochemical pathways involved in the production of many aroma compounds. The expression of these biochemical pathways in yeast often involves the manipulation of the host strain to direct the flux towards certain precursors needed for the production of the given aroma compound. This review highlights recent advances in the bioengineering of yeast -including S. cerevisiae -to produce aroma compounds and bioflavours. To capitalise on recent advances in synthetic yeast genomics, this review presents yeast as a significant producer of bioflavours in a fresh context and proposes new directions for combining engineering and biology principles to improve the yield of targeted aroma compounds.

show abstract

Vanillin Inhibits Translation and Induces Messenger Ribonucleoprotein (mRNP) Granule Formation in Saccharomyces cerevisiae: Application and Validation of High-Content, Image-Based Profiling

Cited by 80 publications

References 39 publications

Plant-derived antifungal agent poacic acid targets β-1,3-glucan

Plant-derived antifungal agent poacic acid targets β-1,3-glucan

The Smell of Synthetic Biology: Engineering Strategies for Aroma Compound Production in Yeast

The Smell of Synthetic Biology: Engineering Strategies for Aroma Compound Production in Yeast

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