Vanilloid Receptor Expression in the Rat Tongue and                Palate

Kido, Mizuho A.; Muroya, Harue; Yamaza, Takayoshi; Terada, Y.; Takano, Teruo

doi:10.1177/154405910308200513

Cited by 92 publications

(59 citation statements)

References 15 publications

(15 reference statements)

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“…The fungiform papillae are rich in TRPV1-immunostained fibers, which are localized around the taste buds, and some of these fibers actually enter taste buds. Most TRPV1 fibers show immunoreactivity for substance P, while approximately half of the TRPV1 fibers are positive for calcitonin gene-related peptide (14,18,21). It has been reported that the neuropeptides CCK and substance P increase rat CT responses to NaCl (13).…”

Section: Possible Interactions Between Trpv1/trpv1t and Trpm5mentioning

confidence: 99%

“…Several agonists and antagonists of TRPV1 modulate the Bz-insensitive NaCl CT responses in animal models and alter human salt taste (11, 20, 26 -30, 40, 41, 48). TRPV1 expression has been observed immunohistochemically in primary afferents and also in oral epithelial cells in the rat tongue (18,21) and in human taste cell lines from lingual epithelium by RT-PCR (16,17). A recent study identified TRPV1 rs8065080 (C Ͼ T) singlenucleotide polymorphism as a likely modifier of an individual's perception of salt at suprathreshold levels (12).…”

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confidence: 99%

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TRPM5-dependent amiloride- and benzamil-insensitive NaCl chorda tympani taste nerve response

Ren

Rhyu

Phan

et al. 2013

American Journal of Physiology-Gastrointestinal and Liver Physi

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Ren Z, Rhyu M, Phan TT, Mummalaneni S, Murthy KS, Grider JR, DeSimone JA, Lyall V. TRPM5-dependent amilorideand benzamil-insensitive NaCl chorda tympani taste nerve response. Am J Physiol Gastrointest Liver Physiol 305: G106 -G117, 2013. First published May 2, 2013; doi:10.1152/ajpgi.00053.2013.-Transient receptor potential (TRP) subfamily M member 5 (TRPM5) cation channel is involved in sensing sweet, bitter, umami, and fat taste stimuli, complex-tasting divalent salts, and temperature-induced changes in sweet taste. To investigate if the amiloride-and benzamil (Bz)-insensitive NaCl chorda tympani (CT) taste nerve response is also regulated in part by TRPM5, CT responses to 100 mM NaCl ϩ 5 M Bz (NaCl ϩ Bz) were monitored in Sprague-Dawley rats, wild-type (WT) mice, and TRP vanilloid subfamily member 1 (TRPV1) and TRPM5 knockout (KO) mice in the presence of resiniferatoxin (RTX), a TRPV1 agonist. In rats, NaCl ϩ Bz ϩ RTX CT responses were also monitored in the presence of triphenylphosphine oxide, a specific TRPM5 blocker, and capsazepine and N-(3-methoxyphenyl)-4-chlorocinnamid (SB-366791), specific TRPV1 blockers. In rats and WT mice, RTX produced biphasic effects on the NaCl ϩ Bz CT response, enhancing the response at 0.5-1 M and inhibiting it at Ͼ1 M. The NaCl ϩ Bz ϩ SB-366791 CT response in rats and WT mice and the NaCl ϩ Bz CT response in TRPV1 KO mice were inhibited to baseline level and were RTX-insensitive. In rats, blocking TRPV1 by capsazepine or TRPM5 by triphenylphosphine oxide inhibited the tonic NaCl ϩ Bz CT response and shifted the relationship between RTX concentration and the magnitude of the tonic CT response to higher RTX concentrations. TRPM5 KO mice elicited no constitutive NaCl ϩ Bz tonic CT response. The relationship between RTX concentration and the magnitude of the tonic NaCl ϩ Bz CT response was significantly attenuated and shifted to higher RTX concentrations. The results suggest that pharmacological or genetic alteration of TRPM5 activity modulates the Bz-insensitive NaCl CT response and its modulation by TRPV1 agonists. triphenylphosphine oxide; resiniferatoxin; capsazepine; SB-366791; TRPV1 APPETITIVE AND AVERSIVE NEURAL and behavioral responses to NaCl are most likely transduced by a variety of mechanisms (35). Several studies suggest that in the anterior tongue Na ϩ from a Na ϩ salt taste stimulus enters a subset of taste bud cells by at least two types of cation channels located in taste cell apical membranes. One channel type is the Na ϩ -specific epithelial Na ϩ channel (ENaC), in which Na ϩ transport is inhibited by amiloride and benzamil (Bz) (1, 3, 8). The second involves Na ϩ transport through a putative transient receptor potential (TRP) vanilloid subfamily member 1 (TRPV1t)-nonspecific cation channel (26,27). The TRPV1t channel is insensitive to amiloride and Bz, permeable to Na ϩ , K ϩ , NH 4 ϩ , and Ca 2ϩ , and contributes to cell depolarization (31). These conclusions are supported by the observations that the phasic (transient) and tonic (steady-state) components of ...

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Section: Possible Interactions Between Trpv1/trpv1t and Trpm5mentioning

confidence: 99%

mentioning

confidence: 99%

TRPM5-dependent amiloride- and benzamil-insensitive NaCl chorda tympani taste nerve response

Ren

Rhyu

Phan

et al. 2013

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…In the urinary bladder, TRPV1 channels are present in both sensory nerves and the bladder epithelium, in which they contribute to voiding behavior (Birder et al, 2001(Birder et al, , 2002. Interestingly, in the tongue, TRPV1-positive fibers innervate the taste papillae (Ishida et al, 2002;Kido et al, 2003), and amiloride-insensitive salt responses in the chorda tympani are abolished in TRPV1-null animals (Lyall et al, 2004). Thus, TRPV1 or a splice variant may transduce some forms of salt taste perception.…”

Section: Trpv1 May Transduce Nociceptive Responses To Elevated Ionic mentioning

confidence: 99%

Extracellular Cations Sensitize and Gate Capsaicin Receptor TRPV1 Modulating Pain Signaling

Ahern¹,

Brooks²,

Miyares³

et al. 2005

J. Neurosci.

164

145

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Transient receptor potential (TRP) channels detect diverse sensory stimuli, including alterations in osmolarity. However, a molecular detector of noxious hypertonic stimuli has not yet been identified. We show here that acute pain-related behavior evoked by elevated ionic strength is abolished in TRP vanilloid subtype 1 (TRPV1)-null mice and inhibited by iodoresiniferatoxin, a potent TRPV1 antagonist. Electrophysiological recordings demonstrate a novel form of ion channel modulation by which extracellular Na ϩ , Mg 2ϩ , and Ca 2ϩ ions sensitize and activate the capsaicin receptor, TRPV1. At room temperature, increasing extracellular Mg 2ϩ (from 1 to 5 mM) or Na ϩ (ϩ50 mM) increased ligand-activated currents up to fourfold, and 10 mM Mg 2ϩ reduced the EC 50 for activation by capsaicin from 890 to 450 nM. Moreover, concentrations of divalent cations Ͼ10 mM directly gate the receptor. These effects occur via electrostatic interactions with two glutamates (E600 and E648) formerly identified as proton-binding residues. Furthermore, phospholipase C-mediated signaling enhances the effects of cations, and physiological concentrations of cations contribute to the bradykinin-evoked activation of TRPV1 and the sensitization of the receptor to heat. Thus, the modulation of TRPV1 by cationic strength may contribute to inflammatory pain signaling.

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“…20) The fact that an intragastric administration of capsiate via a stomach tube increased the energy expenditure in mice provides additional evidence for the oral cavity not being a target of capsinoids to enhance the energy expenditure. 12) There are TRPV1-immunoreactive nerve fibers in both the stomach and small intestine, 21) and almost all these fibers in the small intestine are thought to be derived from extrinsic nerves (spinal and vagus nerves).…”

mentioning

confidence: 99%

Non-Pungent Capsaicin Analogs (Capsinoids) Increase Metabolic Rate and Enhance ThermogenesisviaGastrointestinal TRPV1 in Mice

Kawabata

Inoue

Masamoto

et al. 2009

Bioscience, Biotechnology, and Biochemistry

113

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Vanilloid Receptor Expression in the Rat Tongue and Palate

Cited by 92 publications

References 15 publications

TRPM5-dependent amiloride- and benzamil-insensitive NaCl chorda tympani taste nerve response

TRPM5-dependent amiloride- and benzamil-insensitive NaCl chorda tympani taste nerve response

Extracellular Cations Sensitize and Gate Capsaicin Receptor TRPV1 Modulating Pain Signaling

Non-Pungent Capsaicin Analogs (Capsinoids) Increase Metabolic Rate and Enhance ThermogenesisviaGastrointestinal TRPV1 in Mice

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