Vapor-based coatings for antibacterial and osteogenic functionalization and the immunological compatibility

Wu, Chih‐Yu; Huang, Chao‐Wei; Guan, Zhen‐Yu; Wu, Jyun‐Ting; Yeh, Shu-Yun; Su, Chiao-Tzu; Chang, Chien Hsin; Ding, Shih‐Torng; Chen, Hsien‐Yeh

doi:10.1016/j.msec.2016.06.088

Cited by 8 publications

(6 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unambiguously, these results were found to support the hypothesis that the B. CMC1 bacteria were preserved during the encapsulation and fabrication process. Interestingly, it was discovered that a higher population and a larger enzyme effective zone size were found for the B. CMC1 -encapsulated samples, which indicated their enhanced bacterial growth and cellulase enzymatic activities compared to those of the nonencapsulated sample at the studied time point of 140 h, and it is believed that the highly biocompatible poly- p -xylylene [ 20 , 21 , 22 ] played an important role and provided an agreeable microenvironmental niche for B. CMC1 .…”

Section: Resultsmentioning

confidence: 99%

Synergistic and Regulatable Bioremediation Capsules Fabrication Based on Vapor-Phased Encapsulation of Bacillus Bacteria and its Regulator by Poly-p-Xylylene

Yang

Huang

et al. 2020

Polymers

Self Cite

View full text Add to dashboard Cite

A regulatable bioremediation capsule material was synthesized with isolated single-strain bacteria (Bacillus species, B. CMC1) and a regulator molecule (carboxymethyl cellulose, CMC) by a vapor-phased encapsulation method with simple steps of water sublimation and poly-p-xylylene deposition in chemical vapor deposition (CVD) process. Mechanically, the capsule construct exhibited a controllable shape and dimensions, and was composed of highly biocompatible poly-p-xylylene as the matrix with homogeneously distributed bacteria and CMC molecules. Versatility of the encapsulation of the molecules at the desired concentrations was achieved in the vapor-phased sublimation and deposition fabrication process. The discovery of the fabricated capsule revealed that viable living B. CMC1 inhabited the capsule, and the capsule enhanced bacterial growth due to the materials and process used. Biologically, the encapsulated B. CMC1 demonstrated viable and functional enzyme activity for cellulase activation, and such activity was regulatable and proportional to the concentration of the decorated CMC molecules in the same capsule construct. Impressively, 13% of cellulase activity increase was realized by encapsulation of B. CMC1 by poly-p-xylylene, and a further 34% of cellulase activity increase was achieved by encapsulation of additional 2.5% CMC. Accordingly, this synergistic effectiveness of the capsule constructs was established by combining enzymatic B. CMC1 bacteria and its regulatory CMC by poly-p-xylylene encapsulation process. This reported encapsulation process exhibited other advantages, including the use of simple steps and a dry and clean process free of harmful chemicals; most importantly, the process is scalable for mass production. The present study represents a novel method to fabricate bacteria-encapsulated capsule for cellulose degradation in bioremediation that can be used in various applications, such as wastewater treatment and transforming of cellulose into glucose for biofuel production. Moreover, the concept of this vapor-phased encapsulation technology can be correspondingly used to encapsulate multiple bacteria and regulators to enhance the specific enzyme functions for degradation of various organic matters.

show abstract

Section: Resultsmentioning

confidence: 99%

Synergistic and Regulatable Bioremediation Capsules Fabrication Based on Vapor-Phased Encapsulation of Bacillus Bacteria and its Regulator by Poly-p-Xylylene

Yang

Huang

et al. 2020

Polymers

Self Cite

View full text Add to dashboard Cite

show abstract

“…The switching of GF molecules from FGF-2 to BMP-2 with a conversion rate of 84.0% was estimated by comparing the normalized integrated peak intensities of the −N–H bands (3060–3452 cm –1 ) between the reinstalled BMP-2 surface and the first immobilized BMP-2 surface. With respect to the surface density (concentration) of approximately 150 ng/cm 2 BMP-2 can be estimated from a previous discovery, , and the resulting density on the surface after such cleavage and reinstallation process was calculated with a value 128 ng/cm 2 in the margin of error. The IRRAS spectra of the first immobilized BMP-2 surface are shown in Figure S3.…”

Section: Resultsmentioning

confidence: 99%

Stepwise and Programmable Cell Differentiation Pathways of Controlled Functional Biointerfaces

Guan

Chen

2017

ACS Biomater. Sci. Eng.

Self Cite

View full text Add to dashboard Cite

An advanced control of biomaterial surfaces was created to enable the stepwise and switchable activities of the immobilized growth factor (GF) proteins for a programmed manipulation over cell differentiation pathways. The GF protein was immobilized on an advanced vapor-based coating of poly[(4–2-amide-2′-amine-dithiobisethyl-p-xylylene)-co-(p-xylylene)], and the equipped disulfide exchange mechanism of the coating enables the detachment and/or the displacement of the previously installed GF to reinstall a second GF protein. In this study, the controlled immobilization and displacement of the fibroblast growth factor (FGF-2) and bone morphogenetic protein (BMP-2) were demonstrated on cell culture substrates, and the resulting surfaces provided a programmable induction of cellular responses in proliferation and osteogenesis toward the cultured murine preosteoblasts (MC3T3-E1). A depreciated or stopped activity of the previously induced biological function, i.e., proliferation or osteogenesis, was found for MC3T3-E1 on the modified surface after the cleavage of the corresponding GF. In addition, with the approach to devise the displacement and reinstallation of FGF-2/BMP-2 proteins, a combined induction of proliferation and osteogenesis can be initiated in a timed latency to resolve programmable biological activities.

show abstract

“…A home-built, dual-sourced CVD system was developed that is composed of two independent sublimation/pyrolysis zones and a deposition chamber. A CVD copolymerization mechanism (reported elsewhere) was devised using the CVD system to produce the modification layer of the copolymer, and briefly, NHS ester-substituted para -xylylene and maleimide-substituted para -xylylene (reported elsewhere , ) were utilized as starting materials and synthesized with a 10:1 deposition ratio. The deposition rates were controlled by an mass flow controller (MKS Instruments)-controlled stream of argon that each sublimation/pyrolysis source is equipped with, and in such a case, the argon was controlled at 1.0 Å/s for NHS ester-substituted para -xylylene and 0.1 Å/s for maleimide-substituted para -xylylene.…”

Section: Methodsmentioning

confidence: 99%

“…Detailed staining procedures were conducted following the reported methods. 33 The resultant fluorescence signals were detected also using the IX71 fluorescence microscope, and the images were analyzed also using the ImageJ software. 38 qPCR.…”

Section: ■ Conclusionmentioning

confidence: 99%

Enhanced Growth Activities of Stem Cell Spheroids Based on a Durable and Chemically Defined Surface Modification Coating

Chen

2018

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Surface modification layers are needed for the precise definition of surface chemistries and are equally important for durable and stable adhesive properties to ensure long-term stability and effective performance for biotechnological applications. This study demonstrates a robust modification layer that is synthesized based on chemical vapor deposition copolymerization, and the resultant coating layer is composed of the side-by-side presentation of N-hydroxysuccinimide ester and maleimide functionalities with a controlled ratio to define the immobilization accessibility of chitosan and growth factor protein (FGF-2) molecules on the substrate surface for enhancing cellular activities of stem cells. Characterizations of the copolymer modification layer showed excellent durability, including adhesive strength and thermal stability, and the layer is free of concerns for delamination and/or unacceptable deformation/debris formation that can cause potential toxicity to the surrounding biological environment. Modifications using the copolymer layer on the cell culture surface have demonstrated synergistic activity by chitosan to support the formation of spheroids and by FGF-2 to enhance the proliferation of human adipose-derived stem cells (ADSCs) within the spheroids while increasing the spheroid size and cell numbers. Healthy and flourishing growth activities were discovered for ADSCs on the modified culture surfaces, and the results are useful for potential and related stem cell research and the interfaces of biomaterials.

show abstract

Vapor-based coatings for antibacterial and osteogenic functionalization and the immunological compatibility

Cited by 8 publications

References 63 publications

Synergistic and Regulatable Bioremediation Capsules Fabrication Based on Vapor-Phased Encapsulation of Bacillus Bacteria and its Regulator by Poly-p-Xylylene

Synergistic and Regulatable Bioremediation Capsules Fabrication Based on Vapor-Phased Encapsulation of Bacillus Bacteria and its Regulator by Poly-p-Xylylene

Stepwise and Programmable Cell Differentiation Pathways of Controlled Functional Biointerfaces

Enhanced Growth Activities of Stem Cell Spheroids Based on a Durable and Chemically Defined Surface Modification Coating

Contact Info

Product

Resources

About