Vapor construction and modification of stem cell-laden multicomponent scaffolds for regenerative therapeutics

Chiang, Yu‐Chih; Yeh, Hsiao‐Wen; Hu, Shu‐Man; Wu, Chih‐Yu; Wu, Tai‐Hsi; Chen, Chi‐Hung; Liao, Pei‐Chun; Guan, Zhen‐Yu; Cheng, Nai‐Chen; Chen, Hsien‐Yeh

doi:10.1016/j.mtbio.2022.100213

Cited by 11 publications

(7 citation statements)

References 62 publications

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“…Because the body structure of nonhuman primates is closer to that of humans, 3D-CH-IB-ST needs to be implanted in the TBI model of non-human primates to evaluate the effect of 3D-CH-IB-ST on TBI repair before clinical trials. It is worthwhile to perform further research on the modification of chemical cross-linking and the enhancement of physical qualities in the creation of scaffolds [61][62][63][64][65][66].…”

Section: Discussionmentioning

confidence: 99%

3D printing of injury-preconditioned secretome/collagen/heparan sulfate scaffolds for neurological recovery after traumatic brain injury in rats

et al. 2022

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Background The effects of traumatic brain injury (TBI) can include physical disability and even death. The development of effective therapies to promote neurological recovery is still a challenging problem. 3D-printed biomaterials are considered to have a promising future in TBI repair. The injury-preconditioned secretome derived from human umbilical cord blood mesenchymal stem cells showed better stability in neurological recovery after TBI. Therefore, it is reasonable to assume that a biological scaffold loaded with an injury-preconditioned secretome could facilitate neural network reconstruction after TBI. Methods In this study, we fabricated injury-preconditioned secretome/collagen/heparan sulfate scaffolds by 3D printing. The scaffold structure and porosity were examined by scanning electron microscopy and HE staining. The cytocompatibility of the scaffolds was characterized by MTT analysis, HE staining and electron microscopy. The modified Neurological Severity Score (mNSS), Morris water maze (MWM), and motor evoked potential (MEP) were used to examine the recovery of cognitive and locomotor function after TBI in rats. HE staining, silver staining, Nissl staining, immunofluorescence, and transmission electron microscopy were used to detect the reconstruction of neural structures and pathophysiological processes. The biocompatibility of the scaffolds in vivo was characterized by tolerance exposure and liver/kidney function assays. Results The excellent mechanical and porosity characteristics of the composite scaffold allowed it to efficiently regulate the secretome release rate. MTT and cell adhesion assays demonstrated that the scaffold loaded with the injury-preconditioned secretome (3D-CH-IB-ST) had better cytocompatibility than that loaded with the normal secretome (3D-CH-ST). In the rat TBI model, cognitive and locomotor function including mNSS, MWM, and MEP clearly improved when the scaffold was transplanted into the damage site. There is a significant improvement in nerve tissue at the site of lesion. More abundant endogenous neurons with nerve fibers, synaptic structures, and myelin sheaths were observed in the 3D-CH-IB-ST group. Furthermore, the apoptotic response and neuroinflammation were significantly reduced and functional vessels were observed at the injury site. Good exposure tolerance in vivo demonstrated favorable biocompatibility of the scaffold. Conclusions Our results demonstrated that injury-preconditioned secretome/collagen/heparan sulfate scaffolds fabricated by 3D printing promoted neurological recovery after TBI by reconstructing neural networks, suggesting that the implantation of the scaffolds could be a novel way to alleviate brain damage following TBI. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

3D printing of injury-preconditioned secretome/collagen/heparan sulfate scaffolds for neurological recovery after traumatic brain injury in rats

et al. 2022

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“…229 As another example, poly- p -xylylene porous scaffolds fabricated using ice templating and functionalised with FGF2 and l -ascorbic acid 2-phosphate are shown to enhance MSC proliferation, renewal, differentiation, and in vivo outcomes, including blood vessel ingrowth and induction of osteoblast growth enhancing osteointegration upon implantation in a rat calvarial bone defect, compared to scaffolds lacking one or both bioactive molecules. 230 Similar to injectable materials that increase local oxygen supply for administered MSCs, 3D printed PCL and nanohydroxyapatite scaffolds can also be oxygenated using gelatin-CaO 2 microspheres mixed with the polymer filaments. Compared to non-oxygenated scaffolds, the oxygenated scaffolds exhibit favourable mechanical properties, promote MSC survival in hypoxic conditions, and enhance MSC proliferation and osteogenic differentiation, resulting in enhanced bone regeneration at 4 and 12 weeks after implantation in a rabbit cranial defect.…”

Section: Biomaterials For Msc Transplantationmentioning

confidence: 99%

Cellular modifications and biomaterial design to improve mesenchymal stem cell transplantation

Wong

Mehta

et al. 2023

Biomater. Sci.

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“…3D-printed scaffolds can be customized with specific porosity, pore size and pore connectivity to optimize cell adhesion, migration and nutrient transport [ 92 ]. The 3D porous scaffold poly-p-xylylene (PPX) was implanted in a rat calvarial defect model, and its porous structure greatly promoted the adhesion of hADSCs by providing a highly biocompatible cell channel [ 30 ]. Concurrently, the abundant blood microenvironment provided adequate nutrition for osteogenesis, as evidenced by the discovery of massive bone deposition in the central region of the rat calvarial cavity.…”

Section: Angiogenic Effectmentioning

confidence: 99%

“…Concurrently, the abundant blood microenvironment provided adequate nutrition for osteogenesis, as evidenced by the discovery of massive bone deposition in the central region of the rat calvarial cavity. Particularly, hADSCs are not defined here as cells that directly form new tissues; conversely, they are viewed as ‘medicinal cell factories’ that secrete various BMs in response to the microenvironment and by recruiting endothelial progenitor cells to promote angiogenesis [ 30 ]. Scaffold materials influence ADSCs to exert a pro-angiogenic effect, which is not only related to the structure of materials but also has a substantial relationship with material composition.…”

Section: Angiogenic Effectmentioning

confidence: 99%

Biomaterials combined with ADSCs for bone tissue engineering: current advances and applications

Song,

Wang,

Shi

et al. 2023

Regenerative Biomaterials

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In recent decades, bone tissue engineering, which is supported by scaffold, seed cells and bioactive molecules (BMs), has provided new hope and direction for treating bone defects. In terms of seed cells, compared to bone marrow mesenchymal stem cells, which were widely utilized in previous years, adipose-derived stem cells (ADSCs) are becoming increasingly favored by researchers due to their abundant sources, easy availability and multi-differentiation potentials. However, there is no systematic theoretical basis for selecting appropriate biomaterials loaded with ADSCs. In this review, the regulatory effects of various biomaterials on the behavior of ADSCs are summarized from four perspectives, including biocompatibility, inflammation regulation, angiogenesis and osteogenesis, to illustrate the potential of combining various materials with ADSCs for the treatment of bone defects. In addition, we conclude the influence of additional application of various BMs on the bone repair effect of ADSCs, in order to provide more evidences and support for the selection or preparation of suitable biomaterials and BMs to work with ADSCs. More importantly, the associated clinical case reports and experiments are generalized to provide additional ideas for the clinical transformation and application of bone tissue engineering loaded with ADSCs.

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Vapor construction and modification of stem cell-laden multicomponent scaffolds for regenerative therapeutics

Cited by 11 publications

References 62 publications

3D printing of injury-preconditioned secretome/collagen/heparan sulfate scaffolds for neurological recovery after traumatic brain injury in rats

3D printing of injury-preconditioned secretome/collagen/heparan sulfate scaffolds for neurological recovery after traumatic brain injury in rats

Cellular modifications and biomaterial design to improve mesenchymal stem cell transplantation

Biomaterials combined with ADSCs for bone tissue engineering: current advances and applications

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