Filling post-extraction alveoli with hydroxyapatite-based materials is becoming an increasingly common procedure in veterinary dentistry. In dogs, tooth roots vary in structure depending on the weight of the dog, but data on tooth length and volume have not yet been described. This study aimed to establish reference data on tooth root length and post-extraction alveolar volume for mature maxillary and mandibular incisors and canines in dogs. We determined the mean length and volume of these teeth in dogs in the weight ranges of 1–5 kg, 5–10 kg, 10–20 kg, and over 20 kg. The obtained values given showed a correlation between tooth length and alveolar volume in a specific weight range. A review of the commercially available hydroxyapatite-based bone substitute materials was then conducted. A table is presented which shows how to calculate the volume of bone substitute material required to fill a post-extraction alveolus with a given material. Statistics were used to assess significant differences between the mass of the bone substitute product used (μL) for specific weight ranges and to demonstrate the correlation between tooth length and alveolar volume for a specific weight range. The data obtained in this study can serve as reference values for tooth crown length and alveolar volume, allowing operators to plan a specific volume of bone substitute material for filling post-extraction alveoli. This research is interesting because it shows that the weight of an animal is an important aspect in planning the amount of bone substitute material for tooth extraction. In clinical work, it is much easier to weigh an animal than it is to make calculations based on the length of the tooth root.