Variability and relationships of the Far Eastern species of sculpins Myoxocephalus and Megalocottus (Cottidae) based on mtDNA markers and karyological data

Podlesnykh, A. V.; Морева, И. Н.

doi:10.1134/s1022795414090117

Cited by 11 publications

(16 citation statements)

References 23 publications

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“…However, the present genetic data does not support the synonymization of Megalocottus and Myoxocephalus , as was proposed by a number of authors (e.g., [ 10 , 11 , 69 , 70 , 71 ]). The genetic distance between them is significantly larger than the average interspecific distance within the genus Myoxocephalus (see above) and fits into the range of intergeneric distances detected for sculpin fishes [ 16 , 17 , 18 ], nevertheless, having the lowest values (see also the Results section). Instead, our genetic data indicate that the composition of the genera Myoxocephalus and Megalocottus needs revision.…”

Section: Discussionsupporting

confidence: 68%

Genetic Evidence for a Mixed Composition of the Genus Myoxocephalus (Cottoidei: Cottidae) Necessitates Generic Realignment

Balakirev

Kravchenko

Semenchenko

2020

Genes

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Sculpin fishes belonging to the family Cottidae represent a large and complex group, inhabiting a wide range of freshwater, brackish-water, and marine environments. Numerous studies based on analysis of their morphology and genetic makeup frequently provided controversial results. In the present work, we sequenced complete mitochondrial (mt) genomes and fragments of nuclear ribosomal DNA (rDNA) of the fourhorn sculpin Myoxocephalus quadricornis and some related cottids to increase the power of phylogenetic and taxonomic analyses of this complex fish group. A comparison of the My. quadricornis mt genomes obtained by us with other complete mt genomes available in GenBank has revealed a surprisingly low divergence (3.06 ± 0.12%) with Megalocottus platycephalus and, at the same time, a significantly higher divergence (7.89 ± 0.16%) with the species of the genus Myoxocephalus. Correspondingly, phylogenetic analyses have shown that My. quadricornis is clustered with Me. platycephalus but not with the Myoxocephalus species. Completely consistent patterns of divergence and tree topologies have been obtained based on nuclear rDNA. Thus, the multi-gene data in the present work indicates obvious contradictions in the relationships between the Myoxocephalus and Megalocottus species studied. An extensive phylogenetic analysis has provided evidence for a closer affinity of My. quadricornis with the species of the genus Megalocottus than with the species of the genus Myoxocephalus. A recombination analysis, along with the additional GenBank data, excludes introgression and/or incorrect taxonomic identification as the possible causative factors responsible for the observed closer affinity between the two species from different genera. The above facts necessitate realignment of the genera Myoxocephalus and Megalocottus. The genetic data supports the two recognized genera, Myoxocephalus and Megalocottus, but suggests changing their compositions through transferring My. quadricornis to the genus Megalocottus. The results of the present study resolve the relationships within a complex group of sculpin fishes and show a promising approach to phylogenetic systematics (as a key organizing principle in biodiversity research) for a better understanding of the taxonomy and evolution of fishes and for supplying relevant information to address various fish biodiversity conservation and management issues.

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Section: Discussionsupporting

confidence: 68%

Genetic Evidence for a Mixed Composition of the Genus Myoxocephalus (Cottoidei: Cottidae) Necessitates Generic Realignment

Balakirev

Kravchenko

Semenchenko

2020

Genes

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“…Very low divergence (D xy ¼ 0.0070 ± 0.0030) is also detected between other sculpin species (Figure 1), Comephorus baicalensis (MF346886) and Comephorus dybowskii (MF346888). Moreover, using the mt sequences (COI, CYTB, and 16S rRNA), Podlesnykh and Moreva (2014) and Moreva et al (2017) detected low divergence (1.54 À 2.08%) between M. jaok and Myoxocephalus stelleri, which is significantly lower than the average interspecies value, 0.0380 ± 0.020, obtained previously for sculpin fishes based on the COI marker (Kartavtsev et al 2009). Thus, the 5 0 -COI 'barcode' region is not representative to characterize taxonomic relationships between sculpin fishes.…”

contrasting

confidence: 50%

“…The species is characterized by variable morphology, which makes taxonomic identification difficult. Genetic data for M. jaok are scarce (Podlesnykh and Moreva 2014;Moreva et al 2017); the complete mitochondrial (mt) genome of M. jaok had not yet been sequenced. To increase the power of phylogenetic analysis of this complex fish group we have sequenced two complete mt genomes of M. jaok (GenBank accession numbers MN162694 and MN162695) from the Sea of Japan, Sakhalin Island (sample MJA4-17; Aug-07-2017; 50.89277 N 142.10077 E) and the Sea of Japan, Chikhachyova Bay (sample MJA5-17;Aug-09-2017;51.48575 N 140.8244 E).…”

mentioning

confidence: 99%

Complete mitochondrial genome of the plain sculpin Myoxocephalus jaok (Cottoidei: Cottidae)

Balakirev

Kravchenko

Cherepkova

et al. 2020

Mitochondrial DNA Part B

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“…Cytogenetic and genetic studies of M. stelleri have been described in two publications (Miller 2000;Podlesnykh and Moreva 2014). The only molecular genetic study thus far was based on a small number of specimens from Amur Bay, Sea of Japan, and Odyan Bay, Sea of Okhotsk (Podlesnykh and Moreva 2014). The study highlighted the limitations of using the short fragment of the CO1 mitochondrial gene (525 bp) when constructing an adequate system of the Myoxocephalus species due to the lack of informative characters.…”

Section: Introductionmentioning

confidence: 99%

“…Unlike other Myoxocephalus species from the Sea of Japan and Sea of Okhotsk (Radchenko et al 2020), M. stelleri exhibits chromosomal variation. Karyological analysis of morphologically indistinguishable individuals from Odyan Bay and Amur Bay revealed their differences not only in the diploid numbers (2n) (2n = 44 vs. 2n = 40; fundamental numbers (NF) 44 + 2), but also in the number and localization of active nucleolar organizers (NORs) (Podlesnykh and Moreva 2014). This led the authors to suggest revising the species affiliation of M. stelleri from the Sea of Japan (Podlesnykh and Moreva 2014).…”

Section: Introductionmentioning

confidence: 99%

Differentiation of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 (Actinopterygii, Cottidae) based on mtDNA and karyotype analyses

Морева¹,

Radchenko²,

Petrovskaya³

2021

CCG

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A molecular genetic and karyological study of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 was carried out on an extensive sample from a large area of the species’ range. A total of 42 specimens was sampled from the Sea of Japan, Sea of Okhotsk, and coastal waters off the southern Kuril Islands, which makes this sampling scheme the most comprehensive to date. The level of mtDNA polymorphism was found to be low. The haplotypes of the species formed three phylogenetic groups. The unique M. stelleri haplotype from the coast of Shikotan Island linked all the studied groups, indicating that it is likely ancestral. Robertsonian polymorphism was identified in the species. In all five cytotypes (I – 2n = 44, II – 2n = 43, III – 2n = 42, IV – 2n = 41, V – 2n = 40; NF = 44+2) were identified, all of which were present in the Sea of Japan. Only one (cytotype I) was found in the Sea of Okhotsk, which is probably the closest to the ancestral karyotype. The significant chromosomal polymorphism and the presence of common haplotypes in the studied samples indicate their recent origin from a common ancestor and/or relatively recent contacts within the range. The discrepancies between mtDNA and karyotypes in assigning the ancestral M. stelleri to the coastal waters off Shikotan Island (southern Kuril Islands) and the Sea of Okhotsk, respectively, can be explained by the different inheritance mechanisms and the rates of evolution of molecular genetic and karyological traits.

show abstract

Variability and relationships of the Far Eastern species of sculpins Myoxocephalus and Megalocottus (Cottidae) based on mtDNA markers and karyological data

Cited by 11 publications

References 23 publications

Genetic Evidence for a Mixed Composition of the Genus Myoxocephalus (Cottoidei: Cottidae) Necessitates Generic Realignment

Genetic Evidence for a Mixed Composition of the Genus Myoxocephalus (Cottoidei: Cottidae) Necessitates Generic Realignment

Complete mitochondrial genome of the plain sculpin Myoxocephalus jaok (Cottoidei: Cottidae)

Differentiation of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 (Actinopterygii, Cottidae) based on mtDNA and karyotype analyses

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