Variability at winter wheat varieties first generation which obtained mutagen action

Abstract

The purpose of our investigation was to identify the features of the depressive effects of the aftereffect of different doses of gamma-rays on winter wheat varieties of local breeding in terms of germination, survival, morphometry, yield. The parameters of germination and survival, the passage of the main phases of ontogenesis in winter wheat plants of French varieties (Flamenko and Ghayta) at the first generation were studied. The influence of mutagenic depression on parameters of yield structure (morphometry of mature plants) was established and the level of their variability was estimated too. In 2019–2020, experiments were conducted in the research fields of the research center of the Dnieper State Agrarian and Economic University. The experiments used seeds of Flamenko and Ghayta varieties, irradiated with gamma rays in doses of 100, 150, 200, 250, 300 Gy. Control was dry seeds. The variety Ghayta can be classified as resistant to gamma-rays, the variety Flamenko corresponded to high-sensitive. Parameter of germination and survival were directly correlated with increasing dose, with a dose of 200 Gy already semi-lethal, a dose of 250 Gy for variety Flamenko was sublethal, 300 Gy doze full-lethal, for the variety Ghayta sublethal was 300 Gy dose by which almost no plant material was obtained. Such parameters as plant height, weight of grains from the main spike and weight of thousand grains, weight of grains from the plant, partially number of grain from the main spike reliably reproduce the mutagenic depression. Depression of the plants is affected by the genotype of the variety more than the dose of mutagen; the plant height parameter clearly demonstrates mutagenic depression. According to the results of factor and discriminant analysis as indicators affected by genotype-mutagenic interaction should be used germination and survival, pollen sterility, plant height, grain weight per spike, weight of thousand grains weight of grains from the plant. Doses of 100–200 Gy are optimal for further use to obtain mutations; it is possible to use a dose of 250 Gy for the variety Ghayta.

Determination of Optimum Gamma Ray Irradiation Doses for Hulless Barley (Hordeum vulgare var. nudum L. Hook. f.) Genotypes

“…Gorbatova et al (2020) found that the genetic structure of the varieties was an important factor in their response to low doses of gamma rays. Some other research results indicate that the effects of mutagens may vary according to varieties (Nazarenko and Lykholat, 2020;Hong et al, 2022).…”

Impact of gamma radiation on the agronomic properties of naked barley genotypes

“…Yalin and the line YAA7050-14. F test was used to determine the statistical significance level of the differences, and Duncan's multiple comparison method was used to group the means (Montgomery, 2013).…”

Investigation of Chlorophyll Mutations in Gamma Irradiated Naked Barley Genotypes

“…To achieve sufficient and effective variation in the plant species and cultivars in mutation breeding, it is important to determine the source of the mutagen to be used and the effective dose to be used in advance (Maluszynski et al, 2009) because the effective dose of mutagen depend on the plant species and cultivars (Nazarenko & Lykholat, 2020). In general, the rate of germinated or surviving plants (Ahumada-Flores et al, 2020), the rate of plant developmental retardation (Kodym et al, 2012) and the rate of chlorophyll mutations (Çiftçi & Şenay 2005) are used to determine effective doses and mutation frequencies.…”