Variability in biofilm production by Listeria monocytogenes correlated to strain origin and growth conditions

Nilsson, R; Ross, Tom; Bowman, John P.

doi:10.1016/j.ijfoodmicro.2011.07.012

Cited by 153 publications

(123 citation statements)

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“…While no exopolysaccharidic components have been evidenced in the L. monocytogenes biofilm matrix (8), extracellular DNA (eDNA) has been shown to participate in initial cellular adhesion and biofilm organization under specific growth conditions (10). Biofilm formation by the species is highly dependent on environmental conditions, such as variations in temperature, pH, and nutrients (11,12). L. monocytogenes is structured into four major phylogenetic lineages, each of which is genetically heterogeneous and substructured into highly recognizable clonal complexes as defined by multilocus sequence typing (MLST) (13,14).…”

mentioning

confidence: 99%

Exploring the Diversity of Listeria monocytogenes Biofilm Architecture by High-Throughput Confocal Laser Scanning Microscopy and the Predominance of the Honeycomb-Like Morphotype

Guilbaud

Piveteau

Desvaux

et al. 2015

Appl Environ Microbiol

123

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Listeria monocytogenes is involved in food-borne illness with a high mortality rate. The persistence of the pathogen along the food chain can be associated with its ability to form biofilms on inert surfaces. While most of the phenotypes associated with biofilms are related to their spatial organization, most published data comparing biofilm formation by L. monocytogenes isolates are based on the quantitative crystal violet assay, which does not give access to structural information. Using a high-throughput confocal-imaging approach, the aim of this work was to decipher the structural diversity of biofilms formed by 96 L. monocytogenes strains isolated from various environments. Prior to large-scale analysis, an experimental design was created to improve L. monocytogenes biofilm formation in microscopic-grade microplates, with special emphasis on the growth medium composition. Microscopic analysis of biofilms formed under the selected conditions by the 96 isolates revealed only weak correlation between the genetic lineages of the isolates and the structural properties of the biofilms. However, a gradient in their geometric descriptors (biovolume, mean thickness, and roughness), ranging from flat multilayers to complex honeycomb-like structures, was shown. The dominant honeycomb-like morphotype was characterized by hollow voids hosting free-swimming cells and localized pockets containing mixtures of dead cells and extracellular DNA (eDNA). Listeria monocytogenes still represents an important risk for public health; 1,740 listeriosis cases were reported in the European Union (EU) in 2011 with a mortality rate of 12.7% (1). Listeriosis is particularly dangerous for pregnant women and elderly or immunocompromised people. Persistence of L. monocytogenes strains on food plant surfaces can occur due to maladapted design of equipment and biofilm formation (2, 3). L. monocytogenes is able to attach to and colonize various surfaces, such as stainless steel, glass, and polystyrene, and to contaminate food products during processing (4-6). Biofilms of L. monocytogenes are associated with important ecological advantages, such as protection against biocide action (7). Several molecular determinants, such as flagella, biofilm-associated proteins (Bap), SecA2, and cell-cell communication systems, have been shown to be involved in biofilm construction within the species (8, 9). While no exopolysaccharidic components have been evidenced in the L. monocytogenes biofilm matrix (8), extracellular DNA (eDNA) has been shown to participate in initial cellular adhesion and biofilm organization under specific growth conditions (10). Biofilm formation by the species is highly dependent on environmental conditions, such as variations in temperature, pH, and nutrients (11, 12). L. monocytogenes is structured into four major phylogenetic lineages, each of which is genetically heterogeneous and substructured into highly recognizable clonal complexes as defined by multilocus sequence typing (MLST) (13,14). Attempts to relate biofilm formation ...

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mentioning

confidence: 99%

Exploring the Diversity of Listeria monocytogenes Biofilm Architecture by High-Throughput Confocal Laser Scanning Microscopy and the Predominance of the Honeycomb-Like Morphotype

Guilbaud

Piveteau

Desvaux

et al. 2015

Appl Environ Microbiol

123

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“…flagella-mediated attachment and biofilm initiation), and high temperature (e.g. reduced motility, passive attachment processes, altered cell surface), and each is favored based on the environmental conditions that optimally induce this response in a given strain (Nilsson et al, 2011). In addition sessile bacteria experience multiple micro-environments depending on their location in the spatial structure of the biofilm (Bridier et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The adhesion to the substratum is dependent on the physicochemical properties of the substratum such as texture (rough or smooth), hydrophobicity, and surface charge (Donlan, 2002). In turn, these factors could be modified by other environmental conditions, such as pH, temperature, and nutrient composition of the food matrix (Gerstel and Romling, 2001;Nilsson et al, 2011), as described in the following sections.…”

Section: Chemical Composition Of Substratummentioning

confidence: 99%

“…In contrast to L. monocytogenes clinical strains which produce more biofilm mass at higher temperatures, isolates from food factories increase biofilm formation when temperatures decrease to 10 °C. Although temperature modulates bacterial growth, it is considered that the effect of temperature on biofilm formation is independent of the rate of planktonic growth (Nilsson et al, 2011). Therefore, it is agreed that influence of incubation temperature on biofilm formation occurs through changes in bacterial cells rather than in substratum (Cerca and Jefferson, 2008;Nilsson et al, 2011).…”

Section: Temperaturementioning

confidence: 99%

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Influence of Environmental Factors on Bacterial Biofilm Formation in the Food Industry: A Review

Pagán

García-Gonzalo

2015

PDJ

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Formation and development of bacterial biofilms in the food industry could be a cause of food contamination, compromising food safety and shelf-life. Among the factors modulating biofilm formation, this review will focus in conditions normally encountered by bacteria in food environments, especially in biofilm initiation and development. The effect of environmental factors (substratum, temperature, oxygen concentration, hydrodynamic effects, food matrix composition, and microbial interactions) on biofilm formation is multifaceted and, in many circumstances, their influence could be compensatory. A better knowledge of these factors would allow for a better control of biofilm formation, either by avoiding and/or eradicating biofilms or by defining adequate Hazard Analysis and Critical Control Point systems in the food industry.

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“…Analytical methods based on OD by assessing the final cell density have been used to evaluate the growth of foodborne pathogens in culture media, translucent liquid foods, and diluted food extracts [88][89][90][91]. Data from these studies have offered the possibility to obtain information to determine which food matrices and conditions could be inhibitory of pathogens growth.…”

Section: Applications In Food Matricesmentioning

confidence: 99%

Growth Kinetics for the Selection of Yeast Strains for Fermented Beverages

Miranda-Castilleja¹,

Tapia²,

Arvizu-Medrano³

et al. 2017

Yeast - Industrial Applications

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Criteria to select autochthonous yeast strains for their use in fermented beverages include their ability to dominate the media and to enhance desired sensorial characteristics and their inability to produce undesired compounds such as biogenic amines or off-odors. One of the key features in yeast selection is its Implantation, surpassing different stresses, and its fermentation performance, which requires setting up the process and monitoring it, involving important amount of resources. Methods to evaluate the tolerance of yeast strains are usually based in the qualitative measure of the growth of the microorganism in a medium containing the limiting compound after a specific time of incubation. However, studying strain growth through optical density measurements permits to estimate quantitative and comparable parameters providing an insight into the fitness of the cell to certain environment, lag phase duration, growth rate, and maximum population, among others. In the last decades, cultureindependent methods have been used to evaluate the dynamic of microbial populations during fermentative process. In this chapter, a review of recent advances in the selection of fermentative yeasts as well as the utilization of kinetic evaluation and molecular strategies in conditions associated with fermented beverage for selecting yeast strains is presented.

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Variability in biofilm production by Listeria monocytogenes correlated to strain origin and growth conditions

Cited by 153 publications

References 46 publications

Exploring the Diversity of Listeria monocytogenes Biofilm Architecture by High-Throughput Confocal Laser Scanning Microscopy and the Predominance of the Honeycomb-Like Morphotype

Exploring the Diversity of Listeria monocytogenes Biofilm Architecture by High-Throughput Confocal Laser Scanning Microscopy and the Predominance of the Honeycomb-Like Morphotype

Influence of Environmental Factors on Bacterial Biofilm Formation in the Food Industry: A Review

Growth Kinetics for the Selection of Yeast Strains for Fermented Beverages

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