Variability in capsaicinoid content in different landraces of Capsicum cultivated in north-eastern India

Islam, Aminul; Sharma, Shyam Sundar; Sinha, Pratima; Negi, Madan Singh; Neog, Bijoy; Tripathi, Shashi Bhushan

doi:10.1016/j.scienta.2014.12.011

Cited by 46 publications

(40 citation statements)

References 19 publications

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“…However, only fragmented studies and reports on diversity in capsicum germplasm, which is based on capsaicinoids are currently available [30,31]. Recently, Islam et al (2015) [32] evaluated the levels of variation in capsaicinoid content in 139 diverse accessions using high performance liquid chromatography (HPLC) method. However, the detailed characterization and documentation of capsicum germplasm with respect to morphological traits, pungency, other metabolites, vitamins and their contribution towards antioxidant activities have not been reported till date.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Fruit Metabolites and Pungency Candidate Genes Expression between Bhut Jolokia and Other Capsicum Species

et al. 2016

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Bhut jolokia, commonly known as Ghost chili, a native Capsicum species found in North East India was recorded as the naturally occurring hottest chili in the world by the Guinness Book of World Records in 2006. Although few studies have reported variation in pungency content of this particular species, no study till date has reported detailed expression analysis of candidate genes involved in capsaicinoids (pungency) biosynthesis pathway and other fruit metabolites. Therefore, the present study was designed to evaluate the diversity of fruit morphology, fruiting habit, capsaicinoids and other metabolite contents in 136 different genotypes mainly collected from North East India. Significant intra and inter-specific variations for fruit morphological traits, fruiting habits and 65 fruit metabolites were observed in the collected Capsicum germplasm belonging to three Capsicum species i.e., Capsicum chinense (Bhut jolokia, 63 accessions), C. frutescens (17 accessions) and C. annuum (56 accessions). The pungency level, measured in Scoville Heat Unit (SHU) and antioxidant activity measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay showed maximum levels in C. chinense accessions followed by C. frutescens accessions, while C. annuum accessions showed the lowest value for both the traits. The number of different fruit metabolites detected did not vary significantly among the different species but the metabolite such as benzoic acid hydroxyl esters identified in large percentage in majority of C. annuum genotypes was totally absent in the C. chinense genotypes and sparingly present in few genotypes of C. frutescens. Significant correlations were observed between fruit metabolites capsaicin, dihydrocapsaicin, hexadecanoic acid, cyclopentane, α-tocopherol and antioxidant activity. Furthermore, comparative expression analysis (through qRT-PCR) of candidate genes involved in capsaicinoid biosynthesis pathway revealed many fold higher expression of majority of the genes in C. chinense compared to C. frutescens and C. annuum suggesting that the possible reason for extremely high pungency might be due to the higher level of candidate gene(s) expression although nucleotide variation in pungency related genes may also be involved in imparting variations in level of pungency.

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Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Fruit Metabolites and Pungency Candidate Genes Expression between Bhut Jolokia and Other Capsicum Species

et al. 2016

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“…It clearly indicates that accumulation of capsaicin enhances the accumulation of dihydrocapsaicin irrespective of the genotype. The concentration of capsaicin was found to be higher as compared to that of dihydrocapsaicin in all the Capsicum with high pungency [20]. Therefore, the higher the pungency higher is the content of capsaicin, which has many established pharmacological activity.…”

Section: Economic Prospectmentioning

confidence: 91%

“…In a recent study of 2015, the average capsaicinoid content within 92 bhut jolokia accessions was 41.79 mg/g corresponding to 668,649 SHU. The capsaicinoid content among 92 accessions of bhut jolokia from northeast India varied from 11.95 to 72.05 mg/g with corresponding pungency levels of 191,135 -1,152,832 SHU [20].…”

Section: Phytochemical Profilementioning

confidence: 99%

Capsicum chinense Jacq. (Bhut Jolokia) – rich source of capsaicin with wide application and economic potential

Gogoi

2017

Ann.Pl.Sci.

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Bhut jolokia, which is a cultivar of Capsicum chinense Jacq. is known to the world for its high capsaicinoids content. While wild C. chinense forms may be found in eastern lowland of South America, bhut jolokia is grown in the northeastern states of India. Evidences show that bhut jolokia has interspecific origin with introgression of genes of C. frutecens into C. chinense a natural hybridisation. The fruit of the plant and its leaves has been used as ethnobotanical medicine in different parts of the world. Phytochemical analysis has shown that the fruit is rich in capsaicinoids, which is the reason for its high pungency. Capsaicin and dihydrocapsaicin are the two major capsaicinoids adding pungency to chilli. Higher the capsaicin higher is the pungency. Recent studies has shown wide medicinal applications of capsaicin such as pain relief, anti-obesity treatment, as an antioxidant, antimicrobial agent and even as anticancer molecule. The government of Nagaland has patent rights and geographical indications for naga chilli, which will certainly help in the economic prospect of the region from its cultivation. Thus, this review is an attempt to highlight the latest research and developments in bhut jolokia, which has a huge economic potential to prosper the northeastern region of India.

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“…C. annuum continues to be the most popular and important species, the next being C. frutescens [5,6]. Some varieties are famous for red color because of the pigment capsanthin, others are known for biting pungency attributed by capsaicin [7][8][9]. Paprika (Capsicum spp.)…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genetic diversity in germplasm of paprika (Capsicum spp.) using random amplified polymorphic DNA (RAPD) markers

Renganathan¹

2017

J Plant Sci Phytopathol

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Capsicum spp. is one of the most important economical horticulture crops due to its high consumption either by fresh vegetable or dried spice. Molecular genetic markers offer a number of applications in the genetic improvement of crop plants, which plays an important role in the areas of plant classification and breeding programs. The polygenetic characters of rare species, which are difficult to analyze by traditional methods can, be analyzed easily and classified by using molecular markers. In our study, genetic relationships of twenty-two paprika species were examined to estimate their genetic variations/similarities and to detect the polymorphism present within and among the paprika species by using RAPD-PCR markers. The results revealed that the maximum similarities among the 16 ICBD lines were 100%. The ICBD 03 had 76% similarity compared with other ICBD lines. The CC01 had comparatively low similarity with ICBD forms (30%), followed by EC01 (28%), EC02 (33%), CC02 (35%), and Kt.Pl-19 (60%). The similarity between EC01 and EC02 were 54%. Kt.Pl-19 showed different similarities compared to CC01 (41%), CC02, EC01 (38%), EC02 (29%) and ICBD 03 (40%). The different combinations were tried to optimize the RAPD-PCR profile, which helped to assessing the polymorphism/similarities within and among the Paprika germoplasms were studied. Research ArticleEvaluation of genetic diversity in germplasm of paprika (Capsicum spp.) using random amplifi ed polymorphic DNA (RAPD) markers How to cite this article: Renganathan P, Ruíz-Alvarado C, Hernández-Montiel LG, Duraisamy P, Rueda-Puente EO. Evaluation of genetic diversity in germplasm of paprika (Capsicum spp.) using random amplifi ed polymorphic DNA (RAPD) markers. J

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Variability in capsaicinoid content in different landraces of Capsicum cultivated in north-eastern India

Cited by 46 publications

References 19 publications

Comparative Analysis of Fruit Metabolites and Pungency Candidate Genes Expression between Bhut Jolokia and Other Capsicum Species

Comparative Analysis of Fruit Metabolites and Pungency Candidate Genes Expression between Bhut Jolokia and Other Capsicum Species

Capsicum chinense Jacq. (Bhut Jolokia) – rich source of capsaicin with wide application and economic potential

Evaluation of genetic diversity in germplasm of paprika (Capsicum spp.) using random amplified polymorphic DNA (RAPD) markers

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