Variability in gut mucosal secretory IgA in mice along a working day

Burns, Patricia; Oddi, Sofia; Forzani, Liliana; Tabacman, Eduardo; Reinheimer, Jorge Alberto; Vinderola, Gabriel

doi:10.1186/s13104-018-3213-0

Cited by 10 publications

(7 citation statements)

References 21 publications

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“…Here, we provide evidence of circadian regulation of a major mucosal immune pathway with critical functions in regulating host-microbiota cross-talk, which we hypothesize may have provided evolutionary benefit by balancing the energetic cost of an intestinal immune response with optimal orchestration of microbial mutualism. Specifically, we report diurnal secretion of IgA—in line with previous observations ( 41 , 42 )—and demonstrate feeding as a major cue in aligning rhythms in mucosal antibody secretion. Furthermore, we demonstrate that the absence of IgA significantly affects oscillations in the relative composition and activity of intestinal microbes.…”

Section: Discussionsupporting

confidence: 90%

Rhythmicity of intestinal IgA responses confers oscillatory commensal microbiota mutualism

et al. 2022

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Interactions between the mammalian host and commensal microbiota are enforced through a range of immune responses that confer metabolic benefits and promote tissue health and homeostasis. Immunoglobulin A (IgA) responses directly determine the composition of commensal species that colonize the intestinal tract but require substantial metabolic resources to fuel antibody production by tissue-resident plasma cells. Here, we demonstrate that IgA responses are subject to diurnal regulation over the course of a circadian day. Specifically, the magnitude of IgA secretion, as well as the transcriptome of intestinal IgA + plasma cells, was found to exhibit rhythmicity. Oscillatory IgA responses were found to be entrained by time of feeding and were also found to be in part coordinated by the plasma cell–intrinsic circadian clock via deletion of the master clock gene Arntl . Moreover, reciprocal interactions between the host and microbiota dictated oscillatory dynamics among the commensal microbial community and its associated transcriptional and metabolic activity in an IgA-dependent manner. Together, our findings suggest that circadian networks comprising intestinal IgA, diet, and the microbiota converge to align circadian biology in the intestinal tract and to ensure host-microbial mutualism.

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Section: Discussionsupporting

confidence: 90%

Rhythmicity of intestinal IgA responses confers oscillatory commensal microbiota mutualism

et al. 2022

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“…Here we provide evidence of circadian regulation of a major mucosal immune pathway central to host-microbiota crosstalk, which we hypothesize may have evolved to balance energetic cost with optimal orchestration of microbial mutualism. Specifically, we report diurnal secretion of IgA -in line with previous observations (39,40) -and further define the precise cues and molecular mechanisms that align rhythms in mucosal antibody secretion as well the impact of this response on the microbiota. Our findings suggest a combination of cellintrinsic circadian clocks and cell-extrinsic, feeding-associated nutritional cues entrain rhythms in IgA that modulate oscillations in the commensal microbiota and alter metabolite availability (Fig.…”

Section: Discussionsupporting

confidence: 86%

Rhythmicity of Intestinal IgA Responses Confers Oscillatory Commensal Microbiota Mutualism

Penny

Domingues

Krauss

et al. 2021

Preprint

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Mutualistic interactions with the commensal microbiota are enforced through a range of immune responses that confer metabolic benefits for the host and ensure tissue health and homeostasis. Immunoglobulin (Ig)A responses directly determine the composition of commensal species that colonize the intestinal tract but require significant metabolic resources to fuel antibody production by tissue-resident plasma cells. Here we demonstrate IgA responses are subject to diurnal regulation by dietary-derived metabolic cues and a cell-intrinsic circadian clock. Rhythmicity in IgA secretion conferred oscillatory patterns on the commensal microbial community and its associated metabolic activity, resulting in changes to metabolite availability over the course of the circadian day. Our findings suggest circadian networks comprising intestinal IgA, the diet and the microbiota align to ensure metabolic health.

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“…One reported vaccination study, which examined EspA of the T3SP complex, demonstrated that EspA-specific IgA did not impact cattle-GIT colonization of O157 66 but vaccination may reduce shedding through O157-specific IgG, TLR5-ligation or other unknown mechanism 60,67,68 . Similarly, after experimental challenge, levels of fecal IgA specific to O157 LPS or T3SP were highly variable and generally low 69 , although this level of variation may be dependent on a multitude of host factors which impact GIT transit-time 70,71 . However, as demonstrated here, an induction of O157-specific IgA responses within the GIT post-vaccination may represent one mechanism contributing to reduction in O157 adherence to gut mucosa leading to reduced colonization and subsequent shedding from the reservoir host 38 .…”

Section: Discussionmentioning

confidence: 99%

Cellular and Mucosal Immune Responses Following Vaccination with Inactivated Mutant of Escherichia coli O157:H7

Schaut

Boggiatto

Loving

et al. 2019

Sci Rep

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Shiga toxin-producing Escherichia coli O157:H7 (O157) can cause mild to severe gastrointestinal disease in humans. Cattle are the primary reservoir for O157, which colonizes the intestinal tract without inducing any overt clinical symptoms. Parenteral vaccination can reduce O157 shedding in cattle after challenge and limit zoonotic transmission to humans, although the impact of vaccination and vaccine formulation on cellular and mucosal immune responses are undetermined. To better characterize the cattle immune response to O157 vaccination, cattle were vaccinated with either water-in-oil-adjuvanted, formalin-inactivated hha deletion mutant of Shiga toxin 2 negative (stx2 − ) O157 (Adj-Vac); non-adjuvanted (NoAdj-Vac); or non-vaccinated (NoAdj-NoVac) and peripheral T cell and mucosal antibody responses assessed. Cattle in Adj-Vac group had a higher percentage of O157-specific IFNγ producing CD4 + and γδ + T cells in recall assays compared to the NoAdj-Vac group. Furthermore, O157-specific IgA levels detected in feces of the Adj-Vac group were significantly lower in NoAdj-Vac group. Extracts prepared only from Adj-Vac group feces blocked O157 adherence to epithelial cells. Taken together, these data suggest parenteral administration of adjuvanted, inactivated whole-cell vaccines for O157 can induce O157-specific cellular and mucosal immune responses that may be an important consideration for a successful vaccination scheme.

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Variability in gut mucosal secretory IgA in mice along a working day

Cited by 10 publications

References 21 publications

Rhythmicity of intestinal IgA responses confers oscillatory commensal microbiota mutualism

Rhythmicity of intestinal IgA responses confers oscillatory commensal microbiota mutualism

Rhythmicity of Intestinal IgA Responses Confers Oscillatory Commensal Microbiota Mutualism

Cellular and Mucosal Immune Responses Following Vaccination with Inactivated Mutant of Escherichia coli O157:H7

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