Variability in oxidative stress biomarkers following a maximal exercise test

Abstract

The oxidative stress response to maximal exercise may provide useful clinical biomarkers for assessing redox homeostasis. The aim was to determine the between-individual variability in the exercise-induced change in oxidative stress measures and investigate predictors of these responses. Plasma F2-isoprostanes (Isop), protein carbonyls (PCs), glutathione peroxidase (GPX) activity and total antioxidant capacity (TAC) were measured before and after a maximal treadmill exercise test. Exercise produced significant increases in Isop (27.0%), PC (6.2%) and GPX (7.8%). There were large between-individual coefficients of variation: Isop (152%), PC, (240%), GPX (130%) and TAC (243%).

The effect of Protandim supplementation on oxidative damage and athletic performance.

“…However, males were found to have a higher GPX pre-exercise compared to females, as well as higher post-exercise TAC and GPX values compared to females following a maximal treadmill exercise test 19 . Although there were no significant changes in PC, GPX, and TAC levels between males and females, it is believed there is a large inter-individual difference in exercise-induced changes in oxidative stress and antioxidant biomarkers, suggesting there may be responders and non-responders to exercise induced oxidative stress 19 .…”

“…According to a study by Mullins et al, there were no significant differences in changes in protein carbonyls (PC), GPX, and TAC between females and males following a maximal treadmill exercise test to exhaustion 19 . However, males were found to have a higher GPX pre-exercise compared to females, as well as higher post-exercise TAC and GPX values compared to females following a maximal treadmill exercise test 19 .…”

“…This is a large variability and includes both biological variability and variability of the assay. According to Mullins and colleagues, the between subject-variability in oxidative stress biomarkers is large, suggesting that there may be responders and non-responders to oxidative stress postexercise 19 . The between-individual coefficients of variation were: plasma F2-isoprostanes (Isop) (152%), PC (240%), GPX (130%) and TAC (243%) 19 .…”

“…According to Mullins and colleagues, the between subject-variability in oxidative stress biomarkers is large, suggesting that there may be responders and non-responders to oxidative stress postexercise 19 . The between-individual coefficients of variation were: plasma F2-isoprostanes (Isop) (152%), PC (240%), GPX (130%) and TAC (243%) 19 . In the current study, there were 21 individuals (~55% of the total sample) that experienced increases in oxidative stress from the 5-km race (when averaged over both baseline 5-km sessions).…”

“…Protandim ® supplementation may also impact the overall well-being of the athlete allowing for faster recovery, and a reduced number of injuries 9 . Finally, assessing blood markers of oxidative stress allows evaluation of one's reduction/oxidation ("redox") balance which can identify imbalances and prevent chronic illnesses 19 .…”