In this study, twelve cultivars of potato with different flesh colour (yellow, purple and red) were cultivated in 2012 and 2013 in two trial localities in the Czech Republic and evaluated for the main individual carotenoids. The content of total carotenoids (TC) in analysed cultivars ranged in 1.1-12.2 mg/kg in dry matter (DM) and was influenced by genotype cultivar, locality and year. Cv. Agria (yellow flesh) reached 1.8 to 11.8 times higher levels of TC compared with cultivars of coloured flesh. Locality and year of higher average temperatures during the growing season produced higher TC contents in tubers. Genotype significantly influenced the content and composition of individual carotenoids. As in cv. Agria, violaxanthin (41%) and lutein (55-78%) dominated in all cultivars with coloured flesh. The relative content of β-carotene in cv. Agria represented 2% of TC, in cultivars with coloured flesh 5-12% TC.Keywords: Solanum tuberosum; tuberous crop; xanthophyll; biological antioxidant; variety of potato; weather condition Potatoes are a good source of carotenoids, which are lipophilic compounds synthesized in plastids from isoprenoids (Brown et al. 2008, Ezekiel et al. 2013. The main potato carotenoids are xanthophylls, which are not precursors of vitamin A, but are components on the human retina and prevent cells from damage caused by UV-light. Carotenoids are also effective as biological antioxidants and therefore are probably a key factor in reducing the incidence of many diseases, including cardiovascular diseases, cancer, cataract and macular degeneration (Burgos et al. 2012). Lutein, zeaxanthin, violaxanthin and neoxanthin are the major carotenoids present in potatoes, while β-carotene is present only in trace amounts. The orange and yellow colour of the tuber flesh is mainly due to zeaxanthin and lutein, respectively. Potato cultivars with white flesh contain less carotenoids as compared to cultivars with yellow or orange flesh. Total carotenoids content was reported in the range of 50-350 µg/100 g of fresh matter (FM) and 800-2000 µg/100 g FM, respectively, in white-and yellow-fleshed potato cultivars (Brown et al. 2008).The concentration of carotenoids is affected by several factors such as genotype, agronomic factors, postharvest storage, cooking and processing conditions (Kotíková et al. 2007, Ezekiel et al. 2013, Hejtmánková et al. 2013) and environmental factors (Valcarcel et al. 2015). In recent years there has been a growing interest, especially among fans of a healthy diet, in cultivars with coloured flesh, which are an important source of anthocyanins, and thus popularity of these cultivars is spreading in the world fairly quickly (Lachman et al. 2012, Hamouz et al. 2014. However, data published on their carotenoid contents are absent. The aim of this study was to quantify the level of total and individual carotenoids and to determine the percentage of individual carotenoids in eleven 86 Vol. 62, 2016, No. 2: 86-91 Plant Soil Environ. (Table 1). In the Prague-Uhříněves location the ...