Variability of seed-borne Colletotrichum strains in cotton based on ITS1 and ITS2 ribossomal genes analysis

Abstract

ABSTRACT:The use of DNA sequences analysis has been an important mean to distinguish and to identify populations of organisms at different levels. By molecular markers several complex organisms have been successful detected in plants for distinct aims. Ribosomal DNA (rDNA) has been used to evaluate genetic variability, microorganism phylogeny and to develop specific primers for detection of plant pathogens in plant tissues. In this study, the objective was to characterize isolates of Colletotrichum gossypii var. cephalosporioides and Colletotrichum gossypii, collected in different regions of Brazil, by analyzing the nucleotide sequence of rDNA regions. ITS1, ITS2, and the intervening 5.8S gene were amplified by PCR and their sequences compared to each other and to those from other species registered in the GenBank. The rDNA of isolates associated with Gossypium spp. showed sequence identities ranging from 96 to 100% in the ITS1 region, 98 to 100% in the 5.8S gene, and 97 to 100% in the ITS2 region. The sequences were submitted to UPGMA analysis, and according to the phylogenetic trees, the C. gossypii var. cephalosporioides and C. gossypii species clustered together along with isolates of Glomerella cingulata from mango and papaya, and thus no distinction could be made between isolates of those organisms.

Specificity and sensibility of primer pair in the detection of Colletotrichum gossypii var. cephalosporioides in cotton seeds by PCR technique

“…Traditionally, the identification of that genus' members was based on some morphological characteristics, with emphasis on morphometry of conidia, colony color, mycelial growth rate and pathogenicity (Bailey et al, 1996;Tozze-Júnior et al, 2006). Specifically for the Colletotrichum complex associated with cotton, it is not always possible to differ what are the pathogens involved in the symptomatology of Ramulosis and Anthracnose, as well as the different degrees of aggressiveness and symptoms (Carvalho et al, 2015).…”

“…Accuracy in identification of C. gossypii var. cephalosporioides and C. gossypii is, thus, necessary and indispensable to diagnose and control the involved diseases, as well as demand for detection methods of these fungi in seed samples on laboratory routine activities (Carvalho et al, 2015).…”