Variability of Storage Proteins and Esterase Isozymes in Vicia Sativa Subspecies

Haider, Ashraf; El-Shanshoury, Abd El-Raheem R.

doi:10.1023/a:1002791824350

Cited by 14 publications

(5 citation statements)

References 16 publications

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“…Section Cracca sensu Kupicha ( 1976 ) belongs to subgenus Vicilla. Morphological approach is rather difficult to estimate the all genetic diversity in the genus (Haider and El-Shanshoury 2000 ). Seed proteins are physiologically stable and easy to manipulate (Ladizinsky and Hymowitz 1979 ).…”

Section: Introductionmentioning

confidence: 99%

Ecogeographic variability and genetic diversity associated with seed albumins, globulins and prolamins patterns in Vicia taxa from Algeria

2017

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Genetic variability was studied in 78 populations of locally collected Vicia L. taxa for seed albumins, globulins and prolamins patterns by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) along with an ecogeographic characterization of sites investigated. 131, 119 and 98 bands were respectively used for albumin, globulin and prolamin cluster analysis. Dendrograms based on the Jaccard index and the UPGMA method were generated and the degree of genetic diversity between and within taxa was evaluated. Five clusters were generated from albumins, six from globulins and four from prolamins patterns. The results reflect the great diversity of storage proteins and a high correlation was obtained between the three studied fractions. Several accessions present specific bands which could be used as a discriminatory marker both on intra and interspecific levels. No clear relationships were seen between the groups according to their geographical origin. Data obtained from ecogeographic investigation can be used for future collecting missions.

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Section: Introductionmentioning

confidence: 99%

Ecogeographic variability and genetic diversity associated with seed albumins, globulins and prolamins patterns in Vicia taxa from Algeria

2017

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“…Vicia L. comprises approximately 210 species that are widely distributed in temperate regions of Europe, Asia, and the Americas [2]. Vicia species are morphologically diverse and it is rather difficult to account for the entire genetic variation existing in Vicia species using morphological characters [3]. Archaeological evidence suggests that the Mediterranean region is the principal center of diversification [4].…”

Section: Introductionmentioning

confidence: 99%

Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species

et al. 2015

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Abstract:The temperate and herbaceous genus Vicia L. is a member of the legume tribe Fabeae of the subfamily Papilionoideae. The genus Vicia comprises 166 annual or perennial species distributed mainly in Europe, Asia, and North America, but also extending to the temperate regions of South America and tropical Africa. The use of simple sequence repeat (SSR) markers for Vicia species has not been investigated as extensively as for other crop species. In this study, we assessed the potential for cross-species amplification of cDNA microsatellite markers developed from common vetch (Vicia sativa subsp. sativa). For cross-species amplification of the SSRs, amplification was carried out with genomic DNA isolated from two to eight accessions of 22 different Vicia species. For individual species or subspecies, the transferability rates ranged from 33% for V. ervilia to 82% for V. sativa subsp. nigra with an average rate of 52.0%. Because the rate of successful SSR marker amplification generally correlates with genetic distance, these SSR markers are potentially useful for analyzing genetic relationships between or within Vicia species.

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“…Furthermore, more than 99.4% of the total analyzed varieties have a unique and specific microsatellite profile, allowing the identification of duplicates and closely related varieties, suggesting its potential value and future use in varietal identification. The taxonomy of the Vicia genus is complex, and the exclusive use of agromorphological traits, in some cases, is not sufficient to clarify the genetic relationships between the species of this genus (Haider and El-Shanshoury, 2000). In recent years, an effort has been made to design and use transferable markers to different legume species (Akash and Myers, 2012;Jafari et al, 2013), and emphasis has been done in the cross-species transferability of markers from the V. sativa to other distantly related species of the Vicia genus.…”

Section: Discussionmentioning

confidence: 99%

“…The taxonomy of the Vicia genus is complex, and the exclusive use of agromorphological traits, in some cases, is not sufficient to clarify the genetic relationships between the species of this genus ( Haider and El-Shanshoury, 2000 ). In recent years, an effort has been made to design and use transferable markers to different legume species ( Akash and Myers, 2012 ; Jafari et al, 2013 ), and emphasis has been done in the cross-species transferability of markers from the V .…”

Section: Discussionmentioning

confidence: 99%

Common Vetch, Valuable Germplasm for Resilient Agriculture: Genetic Characterization and Spanish Core Collection Development

et al. 2021

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Common vetch (Vicia sativa L.) is a legume used for animal feed because of its high protein content and great capacity for nitrogen fixation, making this crop relevant in sustainable agriculture. The Spanish vetch collection, conserved at the Spanish Plant Genetic Resources Center (CRF), is one of the largest collections of this species worldwide, including landraces, wild relatives mainly collected in Spain, and commercial cultivars, but also accessions of international origin. The analysis of the genetic diversity of this material, whose genome has not been sequenced yet, and the assembly of a representative collection could play a pivotal role in conserving and exploiting these genetic resources in breeding programs mainly in those focused on consequences and demands of climate change. In this work, a set of 14 simple sequence repeat (SSR) reference alleles for genetic diversity analysis of the CRF vetch collection has been developed, used for genotyping more than 545 common vetch accessions from all over the world and validated. All the tested markers were polymorphic for the analyzed accessions. Overall, at least 86 different loci were identified with 2–11 alleles per locus with an average of 6.1 alleles per locus. Also, the analyses of the generated SSR database support that most of these SSR markers are transferable across closely related species of Vicia genus. Analysis of molecular variance revealed that wild relatives have a higher genetic diversity than landraces. However, cultivars have similar diversity than landraces, indicating that genetic variability has been barely lost due to the breeding of this legume. Low differences of genetic variations between Spanish and non-Spanish accessions have been observed, suggesting a high degree of diversity within Spanish genotypes, which provide 95% of the total genetic variation, so we have focused our efforts on characterizing genotypes of Spanish origin that were further studied using storage protein profiles. Based on SSR, seed protein profiles, and agromorphological and passport data, a vetch core collection (VCC) containing 47 V. sativa accessions of Spanish origin has been established. In this collection, the characterization has been expanded using ISSR markers, and it has been reevaluated with new agromorphological data, including drought tolerance characters. This VCC presents a minimum loss of genetic diversity concerning the total collection and constitutes an invaluable material that can be used in future breeding programs for direct use in a resilient agricultural system.

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Variability of Storage Proteins and Esterase Isozymes in Vicia Sativa Subspecies

Cited by 14 publications

References 16 publications

Ecogeographic variability and genetic diversity associated with seed albumins, globulins and prolamins patterns in Vicia taxa from Algeria

Ecogeographic variability and genetic diversity associated with seed albumins, globulins and prolamins patterns in Vicia taxa from Algeria

Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species

Common Vetch, Valuable Germplasm for Resilient Agriculture: Genetic Characterization and Spanish Core Collection Development

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