Variability of the Mandibular Sclerite and Its Value in Diagnostics of the Third Instar Larvae of the Mediterranean Fruit Fly Ceratitis capitata (Wiedemann, 1824) (Diptera, Tephritidae)

Kamayev, I. O.; Galinskaya, Tatiana V.; Ovtshinnikova, O. G.

doi:10.1134/s0013873820090055

Cited by 2 publications

(3 citation statements)

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“…Several keys for the identification of adult specimens are available ( White and Elson-Harris 1992 ; Virgilio et al 2014 ). Descriptions of larvae of some species are given by White and Elson-Harris 1992 , but other authors give more detailed descriptions of the larvae of some species ( Elson‐Harris 1988 ; Carroll and Wharton 1989 ; Carroll 1998 ; Carroll et al 2004 onwards; Frías et al 2008 ; Lasserre et al 2009 ; Steck and Ekesi 2015 ; Balmes and Mouttet 2017 ; Dutra et al 2018a , b ; Kamayev et al 2020 ; Rodriguez et al 2021 ). However, this information is not always easy to access, and the keys are difficult to follow unless specialized expertise is available.…”

Section: Introductionmentioning

confidence: 99%

“…The presence of a preapical tooth on the mandibles is also variable according to Steck and Ekesi (2015) , while Balmes and Mouttet (2017) use the presence/absence of the preapical tooth as a diagnostic characteristic in their key. Kamayev et al (2020) argue that the preapical tooth is too variable to use as a taxonomic characteristic.…”

Section: Introductionmentioning

confidence: 99%

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Development of a multi-entry identification key for economically important fruit fly larvae (Diptera, Tephritidae, Dacinae)

Pieterse,

De Meyer,

Virgillio

et al. 2024

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Identification of fruit fly larvae is difficult due to the limited morphological characteristics present. However, this is the stage at which fruit flies are intercepted at ports of entry through horticultural imports. Molecular tools are useful but are time-consuming and expensive compared to morphological identifications. This project aims to use available information from the literature and our own research to build a multi-entry identification key for thirteen tephritid species and species groups that are of economic concern for the European Union. Third-instar larvae were obtained from different regions and hosts. Thirteen species or representatives of species groups were obtained, including Ceratitis, Dacus, Bactrocera and Zeugodacus spp. The cephalopharyngeal skeletons were dissected out, cleared in a 10% NaOH solution, dehydrated and mounted in Euparal on glass slides. Images of at least 20 larvae/species were captured using a compound microscope fitted with a camera. Measurements were taken of the mounted mandibles and the number of tubules and their position in the anterior spiracles in relation to the cephalic skeleton were noted. Differences between morphometric parameters were tested via ANOVA and verified using discriminant function analysis. A matrix was compiled including nine characters for which significant inter-specific differentiation was preliminarily detected. The key was converted into a mobile application by LucID.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a multi-entry identification key for economically important fruit fly larvae (Diptera, Tephritidae, Dacinae)

Pieterse,

De Meyer,

Virgillio

et al. 2024

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“…These shortcomings encompass the overlapping ranges of variation among different characters and the omission of accounting for the variability between the right and left mandibular sclerites [29]. Furthermore, recent reports have highlighted the extensive morphological variability in the mandibular sclerites in Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), which can make the morphological identification of tephritid larvae based on this characteristic impossible [31].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Tool for the Identification of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) Using Real-Time PCR

Rizzo,

Zubieta,

Sacchetti

et al. 2024

Insects

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Accurate identification of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), commonly known as the Oriental fruit fly, is a significant challenge due to the morphological convergence and taxonomic uncertainties of species belonging to the same genus. This highly polyphagous species poses a significant threat to fruit crops. With its potential establishment in Europe becoming a growing concern, there is an urgent need for rapid and efficient diagnostic methods. The study presented here introduces a diagnostic protocol based on real-time PCR using a TaqMan probe for the early and reproducible identification of B. dorsalis. Specimens representing the genetic diversity of the Italian population were collected and analyzed. Specific primers and probe were designed based on the conserved regions and an in silico analysis confirmed their specificity. The assay conditions were optimized, and analytical sensitivity, specificity, repeatability, and reproducibility were evaluated. The protocol showed high sensitivity and specificity, accurately detecting low DNA concentrations of B. dorsalis. This standardized method provides a reliable tool for routine diagnostics, enhancing the accuracy and efficiency of identifying the Oriental fruit fly at all stages of its development, thereby facilitating effective pest management measures. The development of this diagnostic protocol is crucial for monitoring and supporting efforts to prevent the passive spread of B. dorsalis in Europe, particularly in light of the recent active infestations detected in Italy.

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Variability of the Mandibular Sclerite and Its Value in Diagnostics of the Third Instar Larvae of the Mediterranean Fruit Fly Ceratitis capitata (Wiedemann, 1824) (Diptera, Tephritidae)

Cited by 2 publications

References 3 publications

Development of a multi-entry identification key for economically important fruit fly larvae (Diptera, Tephritidae, Dacinae)

Development of a multi-entry identification key for economically important fruit fly larvae (Diptera, Tephritidae, Dacinae)

Diagnostic Tool for the Identification of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) Using Real-Time PCR

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