Variability of the prion protein gene (PRNP) in Algerian dromedary populations

Zoubeyda, Kaouadji; Imane, Meghelli; Youcef, Cherifi; Babelhadj, Baaissa; Suheil, Gaouar Semir Bechir; Conte, Michela; Capocefalo, Antonio; Agrimi, Umberto; Chiappini, Barbara; Vaccari, Gabriele

doi:10.1016/j.angen.2020.200106

Cited by 6 publications

(5 citation statements)

References 27 publications

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“…All 50 individuals representing eight breeds of Ethiopian C. dromedarius were monomorphic for the PRNP gene and were 100% identical to the nucleotide sequences reported for the CPD–positive individuals in Algeria ( Babelhadj et al, 2018 ). Most studies ( Abdel-Aziem et al, 2019 ; Adeola et al, 2024 ; Babelhadj et al, 2018 ; Kaluz, Kaluzova & Flint, 1997 ; Tahmoorespur & Jelokhani Niaraki, 2014 ; Tahmoorespur & Jelokhani Niaraki, 2014 ; Xu et al, 2012 ; Zoubeyda et al, 2020 ) report low variability in the PRNP gene among Camelus (0.42% reported herein). Three synonymous nucleotide substitutions (T231C, T243A, and T264C), identified as phylogenetically informative by Parsimony analyses, differentiated the two Camelus species.…”

Section: Discussionmentioning

confidence: 62%

“…Further, one nonapeptide (PQGGGGWGQ) in the N terminal amino acid residue sites followed by four octapeptide (PHGGGWGQ) repeats beginning at codon 54 and ending at codon 94 (spans 41 amino acids) were determined, which is consistent with findings reported in C. dromedarius ( Kaluz, Kaluzova & Flint, 1997 ) and C. bactrianus ( Xu et al, 2012 ). Zoubeyda et al (2020) identified one synonymous substitution (T191T, referenced using traditional amino acid terminology; den Dunnen & Antonarakis, 2000 ), and two nonsynonymous substitutions (G69S and G134E). Further, Adeola et al (2024) observed a one base insertion of a thymine at position 35, which translated to a valine, and a nonsynonymous substitution (G255R).…”

Section: Discussionmentioning

confidence: 99%

“…The lack of nucleotide diversity in PRNP of Camelus may be due to a relatively recent evolutionary history of CPD and therefore has not had sufficient time to produce and accumulate allelic variation and develop a potential resistance to PrP Sc ( Zoubeyda et al, 2020 ). For example, in some domesticated livestock ( i.e., sheep and goats, Goldmann, 2008 ), there is considerable genetic variability (2.51% reported herein) in the PRNP gene where TSEs have been documented since the 1700’s ( Plummer, 1946 ).…”

Section: Discussionmentioning

confidence: 99%

“…Previous genetic studies of dromedary camels in Algeria, Egypt, and Ethiopia ( Cherifi et al, 2017 ; Legesse et al, 2018 ) reported a lack of morphological, genetic variation, and population structure indicating homogeneity in the nuclear genome of C. dromedarius . In addition, low variability of camel PRNP sequences has been reported compared to other sequences representative of dromedary camels ( Abdel-Aziem et al, 2019 ; Babelhadj et al, 2018 ; Kaluz, Kaluzova & Flint, 1997 ; Tahmoorespur & Jelokhani Niaraki, 2014 ; Xu et al, 2012 ; Zoubeyda et al, 2020 ). Given the broad distribution of camel breeds across northern Africa and the apparent lack of genetic variation among breeds, it is hypothesized that Ethiopian dromedary camels will have similar PRNP genotypes to other dromedary camels.…”

Section: Introductionmentioning

confidence: 98%

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Genetic characterization of the prion protein gene in camels (Camelus) with comments on the evolutionary history of prion disease in Cetartiodactyla

Wright,

Reddock,

Roberts

et al. 2024

PeerJ

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Transmissible spongiform encephalopathies (TSEs) are a fatal neurogenerative disease that include Creutzfeldt–Jakob disease in humans, scrapie in sheep and goats, bovine spongiform encephalopathy (BSE), and several others as well as the recently described camel prion disease (CPD). CPD originally was documented in 3.1% of camels examined during an antemortem slaughterhouse inspection in the Ouargla region of Algeria. Of three individuals confirmed for CPD, two were sequenced for the exon 3 of the prion protein gene (PRNP) and were identical to sequences previously reported for Camelus dromedarius. Given that other TSEs, such as BSE, are known to be capable of cross–species transmission and that there is household consumption of meat and milk from Camelus, regulations to ensure camel and human health should be a One Health priority in exporting countries. Although the interspecies transmissibility of CPD currently is unknown, genotypic characterization of Camelus PRNP may be used for predictability of predisposition and potential susceptibility to CPD. Herein, eight breeds of dromedary camels from a previous genetic (mitochondrial DNA and microsatellites) and morphological study were genotyped for PRNP and compared to genotypes from CPD–positive Algerian camels. Sequence data from PRNP indicated that Ethiopian camels possessed 100% sequence identity to CPD–positive camels from Algeria. In addition, the camel PRNP genotype is unique compared to other members of the Orders Cetartiodactyla and Perissodactyla and provides an in–depth phylogenetic analysis of families within Cetartiodactyla and Perissodactyla that was used to infer the evolutionary history of the PRNP gene.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Genetic characterization of the prion protein gene in camels (Camelus) with comments on the evolutionary history of prion disease in Cetartiodactyla

Wright,

Reddock,

Roberts

et al. 2024

PeerJ

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“…Similarly, caprine PRNP SNPs at codons 102, 127, 142, 143, 146, 154, 211, and 222 have been used to assess the vulnerability of goats to scrapie (11,(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)). It's worth noting that no polymorphisms related to this disease have been detected in the PRNP gene in dromedaries (28).…”

Section: Introductionmentioning

confidence: 97%

The first report of polymorphisms of the prion protein gene (PRNP) in Pekin ducks (Anas platyrhynchos domestica)

Jeong,

Wang,

Zou

et al. 2023

Front. Vet. Sci.

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BackgroundPrion diseases have been extensively reported in various mammalian species and are caused by a pathogenic prion protein (PrPSc), which is a misfolded version of cellular prion protein (PrPC). Notably, no cases of prion disease have been reported in birds. Single nucleotide polymorphisms (SNPs) of the prion protein gene (PRNP) that encodes PrP have been associated with susceptibility to prion diseases in several species. However, no studies on PRNP polymorphisms in domestic ducks have been reported thus far.MethodTo investigate PRNP polymorphisms in domestic ducks, we isolated genomic DNA from 214 Pekin duck samples and sequenced the coding region of the Pekin duck PRNP gene. We analyzed genotype, allele, and haplotype distributions and linkage disequilibrium (LD) among the SNPs of the Pekin duck PRNP gene. In addition, we evaluated the effects of the one non-synonymous SNP on the function and structure of PrP using the PROVEAN, PANTHER, SNPs & GO, SODA, and AMYCO in silico prediction programs.ResultsWe found five novel SNPs, c.441 T > C, c.495 T > C, c.582A > G, c.710C > T(P237L), and c.729C > T, in the ORF region of the PRNP gene in 214 Pekin duck samples. We observed strong LD between c.441 T > C and c.582A > G (0.479), and interestingly, the link between c.495 T > C and c.729C > T was in perfect LD, with an r2 value of 1.0. In addition, we identified the five major haplotype frequencies: TTACC, CTGCC, CTACC, CCGCT, and CTATC. Furthermore, we found that the non-synonymous SNP, c.710C > T (P237L), had no detrimental effects on the function or structure of Pekin duck PrP. However, the non-synonymous SNP had deleterious effects on the aggregation propensity and solubility of Pekin duck PrP compared with wildtype Pekin duck PrP.ConclusionTo the best of our knowledge, this study is the first report on the genetic characteristics of PRNP SNPs in Pekin ducks.

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Single nucleotide polymorphisms (SNPs) in the open reading frame (ORF) of prion protein gene (PRNP) in Nigerian livestock species

Adeola,

Bello,

Abdussamad

et al. 2024

BMC Genomics

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Background Prion diseases, also known as transmissible spongiform encephalopathies (TSEs) remain one of the deleterious disorders, which have affected several animal species. Polymorphism of the prion protein (PRNP) gene majorly determines the susceptibility of animals to TSEs. However, only limited studies have examined the variation in PRNP gene in different Nigerian livestock species. Thus, this study aimed to identify the polymorphism of PRNP gene in Nigerian livestock species (including camel, dog, horse, goat, and sheep). We sequenced the open reading frame (ORF) of 65 camels, 31 village dogs and 12 horses from Nigeria and compared with PRNP sequences of 886 individuals retrieved from public databases. Results All the 994 individuals were assigned into 162 haplotypes. The sheep had the highest number of haplotypes (n = 54), and the camel had the lowest (n = 7). Phylogenetic tree further confirmed clustering of Nigerian individuals into their various species. We detected five non-synonymous SNPs of PRNP comprising of G9A, G10A, C11G, G12C, and T669C shared by all Nigerian livestock species and were in Hardy-Weinberg Equilibrium (HWE). The amino acid changes in these five non-synonymous SNP were all “benign” via Polyphen-2 program. Three SNPs G34C, T699C, and C738G occurred only in Nigerian dogs while C16G, G502A, G503A, and C681A in Nigerian horse. In addition, C50T was detected only in goats and sheep. Conclusion Our study serves as the first to simultaneously investigate the polymorphism of PRNP gene in Nigerian livestock species and provides relevant information that could be adopted in programs targeted at breeding for prion diseases resistance.

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Variability of the prion protein gene (PRNP) in Algerian dromedary populations

Cited by 6 publications

References 27 publications

Genetic characterization of the prion protein gene in camels (Camelus) with comments on the evolutionary history of prion disease in Cetartiodactyla

Genetic characterization of the prion protein gene in camels (Camelus) with comments on the evolutionary history of prion disease in Cetartiodactyla

The first report of polymorphisms of the prion protein gene (PRNP) in Pekin ducks (Anas platyrhynchos domestica)

Single nucleotide polymorphisms (SNPs) in the open reading frame (ORF) of prion protein gene (PRNP) in Nigerian livestock species

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