Variability of the wheat genome during in vitro culture

Дубровна, О. В.; Bavol, A. V.

doi:10.3103/s0095452711050033

Cited by 7 publications

(5 citation statements)

References 60 publications

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“…Control of genetic stability is crucial during in vitro culture (Dubrovna and Bavol 2011). PGRs used for micropropagation, especially CKs, integrate into cellular machinery that regulate the cell cycle, and induce undesirable genome size changes (Howell et al 2003;Schaller et al 2014).…”

Section: Resultsmentioning

confidence: 99%

Effective micropropagation of kale (Brassica oleracea convar. Acephala var. sabellica): one of the most important representatives of cruciferous crops

Kamińska

Śliwińska

2023

Plant Cell Tiss Organ Cult

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Kale (Brassica oleracea convar. acephala var. sabellica) is one of the oldest Brassica vegetable varieties with the highest nutritional value and stress resistance. This work aimed to establish an efficient kale micropropagation protocol. Shoot tips were used as explants during axillary shoots multiplication. The most effective cytokinin (6 and 10 shoots per explant after the 1st and 2nd passage, respectively) was 2.5 mg dm−3 6-benzylaminopurine. Rooting was the most effective on MS medium supplemented with 1.0 mg dm−3 indole-3-acetic acid (IAA; 95% rooted shoots). The highest survival rate during acclimatization to ex vitro conditions occurred when plantlets were planted in a soil and perlite mixture. No changes in DNA content were detected using flow cytometry. This paper additionally emphasizes problems associated with the abnormal development of some multiplied Brassicaceae shoots. Nevertheless, the results confirmed that kale micropropagation was successful without any phenotypic aberration.

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Section: Resultsmentioning

confidence: 99%

Effective micropropagation of kale (Brassica oleracea convar. Acephala var. sabellica): one of the most important representatives of cruciferous crops

Kamińska

Śliwińska

2023

Plant Cell Tiss Organ Cult

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“…Результати показали, що всі сорти пшениці мали відмінності за динамікою морфогенезу, що може бути пов'язано з реакцією їхнього генотипу на тривалість дії пониженої температури. Відомо, що для переходу різних сортів озимої пшениці до генеративного стану необхідна різна тривалість яровизації (від 30 до 60 діб) [11,22].…”

Section: ефективність первинного калюсогенезу сортів пшениці м'якої о...unclassified

“…The phytobiotechnology tools i. e. in vitro plant culture have been widely used to create the novel and more advanced soft winter wheat cultivars [11]. Currently, various approaches to investigate the morphogenesis in callus cultures are developed, and the impact of external factors is under study to design the technology for creating the cultivars resistant to adverse environmental conditions [2,15,16].…”

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confidence: 99%

“…Відомо, що ці гени детермінують потребу в яровизації, необхідній для переходу рослин пшениці озимої в генеративний стан [10,18,19]. На даний час досить глибоко досліджені фенотипові ефекти генів VRN на темпи розвитку пшениці озимої [11,22], а також молекулярно-біологічні механізми їхньої експресії [12,13]. Слід зазначити, що вплив генів VRN на морфогенетичні процеси в культурі in vitro практично не вивчений, хоча роботи у цьому напрямі важливі для розуміння механізмів стабільності генетичних систем регуляції розвитку пшениці озимої в умовах порушення цілісності рослинного організму.…”

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Cardioprotective Effect of Low Molecular Weight (Below 5 kDa) Fraction of Cattle Cord Blood Cryohemolysate

Shcheniavsky¹,

Kovalenko²,

Gulevsky³

2022

Probl Cryobiol Cryomed

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In this report, we have studied a cardioprotective effect of low molecular weight (below 5 kDa) fraction derived from cattle cord blood in a rat model of infarction-like cardiovasopathy. The myocardial infarction development was initiated in the animals, maintained for two months on a diet with excessive intake of fat, calcium and sodium salts, by making hypoxia-hypercapnia in them. The use of this model allowed inducing the infarction-like cardiovasopathy, comparable by many respects with the clinical picture of myocardial infarction, which was confirmed by electrocardiography, enzyme-linked immunosorbent assay and histological analysis. Preliminary administration of low molecular weight (below 5 kDa) fraction of cattle cord blood, as well as Actovegin did not prevent the heart attack, but increased the endurance of rats to hypoxia-hypercapnia and reduced the damage rate to myocardium. A preventive administration of low molecular weight fraction of cord blood was proven to mitigate the risk of hypoxia-induced damages to myocardium.

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“…прикладних завдань селекції різних сільськогосподарських рослин і, зокрема, пшениці [16,67]. Особливістю культури соматичних тканин рослин є можливість регенерації повноцінних організмів завдяки властивості тотипотентності рослинної клітини [42,65].…”

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Methods for evaluation of wheat breeding material for drought tolerance

Pykalo¹,

Demydov²,

Yurchenko³

et al. 2020

VLUBS

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Wheat is one of the most valuable cereals on the planet and plays a leading role in the food supply of mankind. The range of wheat is very large, since it is cultivated on five continents in most countries of the world. The genetic improvement of wheat is crucial because of its direct impact on the economic development, international grain trade and food security of the country, so the relevance of research in solving many genetic-breeding problems regarding this crop is growing and acquires a qualitatively new character. The increase in productivity is the most important criterion in the cultivation of any crops, in particular wheat. Drought is one of the main limiting environmental factors that reduce plant productivity. In order to guarantee agriculture from losses in dry years, it is necessary to have varieties tolerant to moisture deficiency. That is why one of the priority areas of wheat breeding is the creation of varieties tolerant to the action of water deficiency. The success of breeding when creating drought tolerant forms largely depends on the correct assessment of the degree of their tolerance. Conducting research on the assessment of genotypes for tolerance to water stress is one of conditions for increasing efficiency of the breeding process of this culture. The results obtained in the analysis of literature data, found that for screening of wheat varieties for drought tolerance there are many methods based on different principles of action, and each of them has its advantages and disadvantages. To accelerate the breeding process and obtain reliable results, it is necessary to apply various methods of researching samples on specific signs of tolerance to stress. The choice of method largely depends on the degree of its complexity, the duration of the assessment and throughput. Therefore, the creation of new and improvement of existing methods for assessing wheat breeding material for drought tolerance in conditions of increasing water deficit or temperature increase will make it possible to objectively characterize the level of adaptability of promising genotypes and predict their behavior in appropriate environmental conditions.

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Variability of the wheat genome during in vitro culture

Cited by 7 publications

References 60 publications

Effective micropropagation of kale (Brassica oleracea convar. Acephala var. sabellica): one of the most important representatives of cruciferous crops

Effective micropropagation of kale (Brassica oleracea convar. Acephala var. sabellica): one of the most important representatives of cruciferous crops

Cardioprotective Effect of Low Molecular Weight (Below 5 kDa) Fraction of Cattle Cord Blood Cryohemolysate

Methods for evaluation of wheat breeding material for drought tolerance

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