Variability of thirteen microsatellite markers in American mink (<i>Mustela vison</i>)

Vincent, I. R.; Farid, A.; Otieno, C. J.

doi:10.4141/a03-001

Cited by 37 publications

(25 citation statements)

References 9 publications

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“…Genetic studies based on mitochondrial DNA sequences have been conducted to identify mustelid species by analysis of DNA extracted from faecal samples (Hansen and Jacobsen 1999;GomezMoliner et al 2004), and to highlight the phylogeography of the European mink (Davison et al 2000), while microsatellite genotyping has been used to survey genetic diversity and population history of M. lutreola populations (Peltier and Lodé 2003;Michaux et al 2004Michaux et al , 2005, of other mustelidae species (M. nigripes, M. eversmanni, M. putorius: Wisely et al 2002) and to investigate variability in captive M. vison (Belliveau et al 1999). A rich library of microsatellite loci exists on M. vison (O'Connell et al 1996;Brusgaard et al 1998a-c, Davis andStrobeck 1998;Fleming et al 1999;Vincent et al 2003;Anistoroaei et al 2006), although not all loci have proved to be polymorphic and informative or have been tested on populations. Up to now, genetic information on feral American mink populations introduced in Europe is scarce or completely lacking.…”

Section: Introductionmentioning

confidence: 99%

Population genetic structure and distribution of introduced American mink (Mustela vison) in Spain, based on microsatellite variation

et al. 2007

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The population genetic structure of an invasive species in Spain, the American mink (Mustela vison), was investigated using microsatellite DNA markers. This semiaquatic carnivore, originating from North America, was imported into Europe for fur farming since the beginning of the 20th century. Due to massive escapes, farm damages, deliberate releases and/or accidents, feral mink populations were established in the aquatic ecosystems of many European countries, including Spain. We genotyped 155 American mink originating from the Spanish regions Basque Country, Catalonia, Castilla-Leon, Aragon, Valencia and Galicia using 10 polymorphic microsatellite loci to highlight population genetic structure, distribution and dispersal. M. vison populations in Spain appear differentiated and not yet connected by gene flow. Bayesian clustering analyses and spatial analyses of molecular variance detected four inferred clusters, overall coinciding with the sampled geographical localities. Preliminary testing shows moderate to large estimated effective population sizes. Molecular analyses result useful to provide baseline data for further research on the evolution of invasive mink populations, as well as support local management strategies and indirectly benefit the conservation of threatened species in Spain, such as the endangered European mink (Mustela lutreola), and the polecat (Mustela putorius), which share the habitat with the American mink.

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Section: Introductionmentioning

confidence: 99%

Population genetic structure and distribution of introduced American mink (Mustela vison) in Spain, based on microsatellite variation

et al. 2007

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“…These were from Gulo gulo (Gg7, Gg454 and Ggu234; Davis and Strobeck 1998;Duffy et al 1998;Walker et al 2001), Lutra lutra (Lut615, Lut604 and 04OT14; Dallas and Piertney 1998;Huang et al 2005), M. americana (Ma1, Ma2, Ma5 and Ma8; Davis and Strobeck 1998), Meles meles (Mel1, Mel6 and Mel105; Bijlsma et al 2000;, Mustela erminea (Mer041; Fleming et al 1999), Mustela lutreola (MLUT27; Cabria et al 2007) and Mustela vison (Mvi1341, Mvi1354, Mvis072, Mvis075 and Mvis020; Fleming et al 1999;Vincent et al 2003). Microsatellite amplifications were performed in a total volume of 15 µl with 1 µl DNA extract, 1.5 mM MgCl 2 , 0.2 µM each primer, 0.2 mM dNTP, 1X GeneAmp PCR Buffer (Applied Biosystems) and 1 U of AmpliTaq Gold™ polymerase (Applied Biosystems).…”

Section: Microsatellite Analysismentioning

confidence: 98%

Remotely plucked hair genotyping: a reliable and non-invasive method for censusing pine marten (Martes martes, L. 1758) populations

et al. 2009

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We investigated the feasibility of using genetic techniques to census pine marten (Martes martes) populations by genotyping non-invasively collected samples (plucked hair and scats), with particular reference to the genetically depauperate Irish population. Novel real-time polymerase chain reaction methods were developed for species and sex identification, targeting short DNA sequences. Background genetic variation at 17 microsatellite loci was very low in the Irish population, with an average of 2.29 alleles per locus and expected heterozygosity of 0.35. Despite such low polymorphism, a panel of eight loci with a sibling probability of identity of 0.011 reliably identified individual pine marten and their gender, as determined by reference to genotypes of live trapped individuals. With high nuclear DNA amplification success rates (93.8%) and low genotyping error rates (1.8%), plucked hairs may represent a more reliable and cost-effective DNA source than scats for monitoring populations of this elusive carnivore, and similar taxa such as the sympatric stone marten Martes foina.

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“…Muscle samples were taken from the carcasses, and DNA was extracted by using Chelex Ò 5%. In a first step, ten pairs of microsatellite primers, containing up to seven alleles, were selected from Fleming et al (1999) and Vincent et al (2003). Six loci were polymorphic (Mvi1321, Mvi1322, Mvi1302, Mvi1341, Mvi1273, Mvi1272 meant up to seven alleles per locus) and were scored on the total samples.…”

Section: Dna Extraction Amplification and Genotypingmentioning

confidence: 99%

Evidence of admixture between differentiated genetic pools at a regional scale in an invasive carnivore

et al. 2009

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Invasive species represent a major threat to biodiversity, and the understanding of their population genetics is one of the most important goals in conservation biology. Recently, it has been proposed that methods using molecular tools could help define efficient eradication strategies and should be a preliminary step in the management process. The American mink was introduced in Europe for fur farming purposes in the 1920s, and, due to escapees, several feral populations have been mentioned in the last decades. In France, feral mink have been observed since the 1970s, and the largest population, located in Brittany, is considered to be still expanding. We investigated the genetic variability and population structure of 149 feral mink and 21 farmed mink from this area using six microsatellite loci. Our results showed three genetically distinct population units at the regional scale. A pattern of isolation by distance was observed for the whole sample. In our case we explain this pattern by recent admixture of the three genetic units. Our findings suggest that populations have recently met and started to homogenise.

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Variability of thirteen microsatellite markers in American mink (Mustela vison)

Cited by 37 publications

References 9 publications

Population genetic structure and distribution of introduced American mink (Mustela vison) in Spain, based on microsatellite variation

Population genetic structure and distribution of introduced American mink (Mustela vison) in Spain, based on microsatellite variation

Remotely plucked hair genotyping: a reliable and non-invasive method for censusing pine marten (Martes martes, L. 1758) populations

Evidence of admixture between differentiated genetic pools at a regional scale in an invasive carnivore

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