Variability study of entomopathogenic nematode populations (Heterorhabditidae) from Argentina

Achinelly, María Fernanda; Eliceche, Daiana Pamela; Belaich, Mariano Nicolás; Ghiringhelli, Pablo Daniel

doi:10.1590/1519-6984.20015

Cited by 13 publications

(6 citation statements)

References 11 publications

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“…These variations were found to be independent of the developmental stages and the collection localities of soil samples. It was evident from the previous studies that there were large intraspeci c variations present among the nematodes which were also observed among present isolates of the present investigation (Achinelly et al, 2017;Dolinski et al, 2008). However, less intraspeci c variations were observed between CH25 and CH26 as they formed groups (ellipses) in all studied stages.…”

Section: Discussionsupporting

confidence: 76%

Characterization of Heterorhabditis indica (Nematoda: Rhabditida) and their efficacy against insect pests by using regression probit analysis model: Implication on biological control of pest management

Bhargava

Sharma

Chaubey

2022

Preprint

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The potential of Entomopathogenic nematodes (EPN) under laboratory conditions against insect pests has been known worldwide. In the present investigation, the isolates were recovered from the Indian soils and the susceptibility of Galleria mellonella and Spodoptera litura to the infection of EPN was examined. Among all the collected 360 soil samples, four soil samples were found to be positive for the EPN strains and docketed as CH22, CH23, CH25 and CH26.The isolated strains were identified as Heterorhabditis indica based on the morphology, morphometry and phylogenetic analysis. Only one species of Heterorhabditis has been recovered from the Indian soils which could be inferred as one of the dominant species of EPN in Uttar Pradesh, India. The principal component analysis (PCA) values showed large intraspecific differences between the isolated strains and interspecific differences between the species of the Indica group. The laboratory bioassays against the G.mellonella and S.litura revealed the highest virulent strain in CH23 and the least virulence were observed in CH26. The median lethal concentration of CH23 isolate obtained from the bioassays showed high virulence against G. mellonella (LC50 =22.688) and S. litura (LC50 =76.454) within 36 hrs. The CH23 and CH26 isolates showed a positive correlation between the dose and % mortality against both pests.The CH23 strain of H. indica showed high virulence and can be exploited as a new bio-control agent against the insect pest in the integrated pest management programmes (IPM).

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Section: Discussionsupporting

confidence: 76%

Characterization of Heterorhabditis indica (Nematoda: Rhabditida) and their efficacy against insect pests by using regression probit analysis model: Implication on biological control of pest management

Bhargava

Sharma

Chaubey

2022

Preprint

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“…Synonymized by Hominick (2002) based on molecular evidence provided by Adams et al (1998) . Synonymization status is supported by molecular data of Phan et al (2003) and Achinelly et al (2017) .…”

Section: Resultsmentioning

confidence: 68%

Multi-locus phylogenetic analyses uncover species boundaries and reveal the occurrence of two new entomopathogenic nematode species, Heterorhabditis ruandica n. sp. and Heterorhabditis zacatecana n. sp.

Machado

Bhat²,

Abolafia

et al. 2021

Journal of Nematology

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Species of the nematode genus Heterorhabditis are important biological control agents against agricultural pests. The taxonomy of this group is still unclear as it currently relies on phylogenetic reconstructions based on a few genetic markers with little resolutive power, specially of closely related species. To fill this knowledge gap, we sequenced several phylogenetically relevant genetic loci and used them to reconstruct phylogenetic trees, to calculate sequence similarity scores, and to determine signatures of species- and population-specific genetic polymorphism. In addition, we revisited the current literature related to the description, synonymisation, and declaration as species inquirendae of Heterorhabditis species to compile taxonomically relevant morphological and morphometric characters, characterized new nematode isolates at the morphological and morphometrical level, and conducted self-crossing and cross-hybridization experiments. The results of this study show that the sequences of the mitochondrial cytochrome C oxidase subunit I (COI) gene provide better phylogenetic resolutive power than the sequences of nuclear rRNA genes and that this gene marker can phylogenetically resolve closely related species and even populations of the same species with high precision. Using this gene marker, we found two new species, Heterorhabditis ruandica n. sp. and Heterorhabditis zacatecana n. sp. A detailed characterization of these species at the morphological and morphometric levels and nematode reproduction assays revealed that the threshold for species delimitation in this genus, using COI sequences, is 97% to 98%. Our study illustrates the importance of rigorous morphological and morphometric characterization and multi-locus sequencing for the description of new species within the genus Heterorhabditis, serves to clarify the phylogenetic relationships of this important group of biological control agents, and can inform future species descriptions to advance our efforts towards developing more tools for sustainable and environmentally friendly agriculture.

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“…This is consistent with the findings of Morales et al (2016), who observed differences between Venezuelan populations of H. amazonensis, and Poinar (1993) and Stock et al (1996), who reported intraspecific differences among species of Steinernematidae. Achinelly et al (2017), evaluating the morphometric characteristics of Heterorhabditis bacteriophora VELI and H. argentinensis isolated from different regions of Argentina, observed that the isolates obtained in regions with more rainfall and humidity had larger dimensions. According to these authors, the juvenile phase (JIs) has the least variability and is the best option for the morphological comparison of different Heterorhabditis populations.…”

Section: Morphometric Characterizationmentioning

confidence: 99%

First report and biological characteristics of Heterorhabditis amazonensis in the state of Paraná, Brazil

et al. 2024

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Entomopathogenic nematodes (EPNs) from Heterorhabditidae and Steinernematidae families are extensively used to control insect pests. In Brazil, however, relatively few studies have identified and characterized these entomopathogens. The objective of this study was to identify and characterize an EPN isolate obtained from soil samples collected in the state of Paraná, Brazil. An isolate (UEL 08) of Heterorhabditis was detected in a soil sample collected from a pasture area cultivated with Brachiaria grass in Londrina, state of Paraná, Brazil (23°34ʹ311ʹʹS, 050°58ʹ298ʹʹW), using the insect-baiting technique with Galleria mellonella larvae as hosts. The nematode was identified through morphometric studies and molecular analyses based on amplification of the rDNA ITS region. Although we identified certain morphometric differences compared with the original description, the molecular data indicated that the ITS sequence obtained for the UEL 08 isolate is identical to the reference sequence of H. amazonensis (DQ665222) and presented 100% similarity. Thus, the findings of our morphological and molecular studies confirmed that the isolated nematode is H. amazonensis, which is the first time this species has been registered in Paraná. Study of the biological characteristics of H. amazonensis (UEL 08) revealed that the isolate has two distinct life cycles – one short (216 h) and the other long (288 h) – and produces two generations in both cycles. We observed that H. amazonensis (UEL 8) was pathogenic and virulent to the three evaluated hosts, although with different virulence against these hosts. The larvae of G. mellonella and Alphitobius diaperinus were more susceptible than adult Dichelops (Diacereus) melacanthus, with 100%, 85%, and 46% mortality, respectively. Furthermore, an in vivo production assay revealed a mean daily yield of 3.4 × 103 infective juveniles/g host larvae.

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Variability study of entomopathogenic nematode populations (Heterorhabditidae) from Argentina

Cited by 13 publications

References 11 publications

Characterization of Heterorhabditis indica (Nematoda: Rhabditida) and their efficacy against insect pests by using regression probit analysis model: Implication on biological control of pest management

Characterization of Heterorhabditis indica (Nematoda: Rhabditida) and their efficacy against insect pests by using regression probit analysis model: Implication on biological control of pest management

Multi-locus phylogenetic analyses uncover species boundaries and reveal the occurrence of two new entomopathogenic nematode species, Heterorhabditis ruandica n. sp. and Heterorhabditis zacatecana n. sp.

First report and biological characteristics of Heterorhabditis amazonensis in the state of Paraná, Brazil

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