Variable allelopathy among phytoplankton reflected in red tide metabolome

Poulin, Remington X.; Poulson‐Ellestad, Kelsey L.; Roy, Jessie S.; Kubanek, Julia

doi:10.1016/j.hal.2017.12.002

Cited by 34 publications

(13 citation statements)

References 37 publications

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“…The best-studied K. brevis metabolites, brevetoxins, did not emerge as the chemicals differentiating high bloom from low bloom sites, and in fact were not detected in the metabolomic analysis, likely because K. brevis compounds were lost due to cell lysis. In addition to brevetoxins, K. brevis produces a suite of allelopathic compounds (Prince et al, 2010;Poulin et al, 2018b) which have recently been linked to metabolic impacts on phytoplankton competitors (Poulson-Ellestad et al, 2014;Poulin et al, 2018a). This allelopathy and resulting changes in phytoplankton community structure could be partially responsible for the discrete clustering of high versus low bloom lipid-soluble metabolomes in both the small and intermediate size fractions (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The pool of bioactive metabolites accompanying a bloom may also influence the presence and abundance of surrounding microbes. K. brevis produces a wide array of compounds in addition to brevetoxins; indeed, many metabolites remain unidentified including those responsible for most of the allelopathy against other phytoplankton species ( Poulson-Ellestad et al, 2014 ; Poulin et al, 2018b ). Bioactive metabolites may inhibit microbes either directly (through antimicrobial activity) or indirectly (by changing the plankton community structure and nutrient availability).…”

Section: Discussionmentioning

confidence: 99%

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Microbial and chemical dynamics of a toxic dinoflagellate bloom

Patin

Brown

Chebli

et al. 2020

PeerJ

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Harmful Algal Blooms (HABs) exert considerable ecological and economic damage and are becoming increasingly frequent worldwide. However, the biological factors underlying HABs remain uncertain. Relationships between algae and bacteria may contribute to bloom formation, strength, and duration. We investigated the microbial communities and metabolomes associated with a HAB of the toxic dinoflagellate Karenia brevis off the west coast of Florida in June 2018. Microbial communities and intracellular metabolite pools differed based on both bacterial lifestyle and bloom level, suggesting a complex role for blooms in reshaping microbial processes. Network analysis identified K. brevis as an ecological hub in the planktonic ecosystem, with significant connections to diverse microbial taxa. These included four flavobacteria and one sequence variant unidentified past the domain level, suggesting uncharacterized diversity in phytoplankton-associated microbial communities. Additionally, intracellular metabolomic analyses associated high K. brevis levels with higher levels of aromatic compounds and lipids. These findings reveal water column microbial and chemical characteristics with potentially important implications for understanding HAB onset and duration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Microbial and chemical dynamics of a toxic dinoflagellate bloom

Patin

Brown

Chebli

et al. 2020

PeerJ

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“…K . brevis allelopathy is, however, known to be highly variable and does not typically lead to devastating effects on cell growth 51 . The integration of metabolomics involving both polar and non-polar classes with proteomics analysis suggests that K .…”

Section: Discussionmentioning

confidence: 99%

Karenia brevis allelopathy compromises the lipidome, membrane integrity, and photosynthesis of competitors

Poulin

Hogan

Poulson‐Ellestad

et al. 2018

Sci Rep

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The formation, propagation, and maintenance of harmful algal blooms are of interest due to their negative effects on marine life and human health. Some bloom-forming algae utilize allelopathy, the release of compounds that inhibit competitors, to exclude other species dependent on a common pool of limiting resources. Allelopathy is hypothesized to affect bloom dynamics and is well established in the red tide dinoflagellate Karenia brevis. K. brevis typically suppresses competitor growth rather than being acutely toxic to other algae. When we investigated the effects of allelopathy on two competitors, Asterionellopsis glacialis and Thalassiosira pseudonana, using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS)-based metabolomics, we found that the lipidomes of both species were significantly altered. However, A. glacialis maintained a more robust metabolism in response to K. brevis allelopathy whereas T. pseudonana exhibited significant alterations in lipid synthesis, cell membrane integrity, and photosynthesis. Membrane-associated lipids were significantly suppressed for T. pseudonana exposed to allelopathy such that membranes of living cells became permeable. K. brevis allelopathy appears to target lipid biosynthesis affecting multiple physiological pathways suggesting that exuded compounds have the ability to significantly alter competitor physiology, giving K. brevis an edge over sensitive species.

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“…For instance, this technique allowed the quantitative identification of the lipopeptide malyngamide C by different strains of cyanobacteria (Winnikoff et al, 2014). More and more studies are applying metabolomic approaches to investigate biotic interactions between HAB species and their environment (Giacometti et al, 2013;Poulson-Ellestad et al, 2014;Poulin et al, 2018). The review of McLean (2013) on "omics" tools demonstrates the relevance of genomics, transcriptomics and proteomics approaches to advance our understanding on the ecology of HABs.…”

Section: Use Of Metabolomics and Other «Omics» Toolsmentioning

confidence: 99%