Variable (dG-dT) ·(dC-dA)  sequences in the porcine genome

Winterø, A. K.; Fredholm, Merete; Thomsen, Preben D.

doi:10.1016/0888-7543(92)90375-3

Cited by 102 publications

(44 citation statements)

References 29 publications

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“…The PCR amplification products are then examined and sized after electrophoresis on agarose or polyacrylamide gels. In all investigated species, microsatellites have been found to be highly variable among individuals (Hamada et a!., 1982;Hearne et al, 1992;Wintero et al, 1992;Estoup et a!., 1993;Hughes & Queller, 1993). The high degree of polymorphism and random distribution in the genome make microsatellite loci promising candidates for use as genetic markers in a large scope of genetic studies such as linkage analysis, genome mapping, population structure and stock composition (Estoup et al, 1993;Hughes & Queller, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Protein and microsatellite single locus variability in Salmo salar L. (Atlantic salmon)

et al. 1996

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We describe genetic variation at three microsatellite single loci and six allozyme loci of seven natural Atlantic salmon populations from Ireland and Spain. A comparison of genetic variability detected at both types of loci is performed. Also, the relative value of microsatellite single locus variability with regard to the identification of individual salmon populations is assessed. Microsatellite loci are shown to display higher levels of variation than allozyme loci.Mean number of alleles (6 1.53) and heterozygosity (0.46 0.04) at microsatellite loci are greater than those found for allozymes (1.85±0.05 and 0.21±0.03, respectively), and some microsatellite alleles appear to be specific for a location or geographical area. Allozyme and microsatellite variation show the same pattern of differentiation between populations with Irish and Spanish populations grouped into different clusters. However, greater values of genetic distance were found among microsatellite (D = 0.0747±0.011) rather than among allozyme loci (D = 0.0449 0.008). These results provide evidence that microsatellite-based analysis of genetic variation will be useful in the identification of individual populations of Atlantic salmon.

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Section: Introductionmentioning

confidence: 99%

“…One type of repetitive element, referred to as microsatellites, corresponds to tandemly repeated DNA motifs of one to five base pairs in length evenly distributed in the genome of all investigated eukaryotic species (Hamada et a!., 1982;Weber, 1990;Wong et a!., 1990;Wintero et a!., 1992; Estoup et al, 1993;Slettan et a!., 1993).…”

Section: Introductionmentioning

confidence: 99%

Protein and microsatellite single locus variability in Salmo salar L. (Atlantic salmon)

et al. 1996

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“…488 centromeric and telomeric regions. Microsatellite sequences correspond to tandemly repeated motifs of one to five base pairs for a total length usually lower than 0.2 kb (Rassman et al, 1991); they seem to be more randomly distributed along the chromosomes than minisatellites (Hearne eta!., 1992), but are underrepresented in the centromeric and telomeric regions of chromosomes (Wong et a!., 1990;Wintero et a!., 1992). Both kinds of tandemly repeated sequences have been found in all the eukaryotic genomes so far studied (Epplen etal., 1991;Jarman & Wells, 1989).…”

Section: Introductionmentioning

confidence: 99%

(CT)n and (GT)n microsatellites: a new class of genetic markers for Salmo trutta L. (brown trout)

et al. 1993

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Thirteen (GT ) and four (CT ), microsatellite loci (n = 10 or more and n =20 or more, respectively) have been isolated from a partial genomic library of brown trout and sequenced. On average, a (GT ) repeat sequence occurs approximately every 23 kb and a (CT ) repeat sequence every 76 kb in brown trout genome. Primers for DNA amplifications using the polymerase chain reaction (PCR) were synthesized for three single locus microsatellites. Mendelian inheritance of the observed polymorphisms was confirmed in full-sib families. Four brown trout populations (10 unrelated individuals per population) were screened for polymorphism with these three microsatellite loci. The total number of alleles detected in the four populations is five at one locus, six at the other two microsatellite loci and is three, on average, per population. Heterozygosities range from 0.18 to 0.74. The largest differences in allelic frequencies occurred between the Mediterranean and the Atlantic populations: this result is congruent with previous allozymic data. The gene-centromere distances of the three microsatellite markers were determined on gynogenetic lines: post-reduction rates range from 0.17 to 0.60. For all the three microsatellite loci, the primers designed from brown trout sequences can be used in another closely related species of salmonid, the rainbow trout (Oncorhynchus mykiss). This last aspect supports the view that microsatellite markers may have wide application in genetic studies in salmonid species and fishes in general.

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“…Microsatellites are highly polymorphic co-dominant markers with simple identification, low mutation rates (Ellegren 1995) and uniform distribution throughout genome (Wintero et al 1992). Microsatellite markers have been successfully used to assess the genetic structure and genetic diversity of breeds of domestic animals (Hall & Bradley 1995, Wimmers et al 1999, Aberle & Distl 2004, Switonski 2004, Zsolnai et al 2006, Kadlecik et al 2004, Schwarz et al 2005, Kasarda & Kadlecik 2007, Slaska et al 2008, except the genetic aspects also influence of environmental factors were observed in Slovak pig breeds , Petrovic et al 2009, Marcincak et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Genetic admixture in pig population observed by microsatellite markers

Židek

Jakabová²,

Trandžík

et al. 2011

Arch. Anim. Breed.

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The aim of presented study was estimation of genetic relationship within and between 3 mentioned pig breeds bred in the Slovak Republic. Genetic variability at 10 microsatellite loci (SW24, SO107, SO068, SW936, sw353, so386, so355, sw72, tnfb, and SO070) was analysed in 412 pigs of Landrace, Yorkshire and Slovak White Improved breeds. Variation amount of each population was measured with average number of alleles per locus, heterozygosities, polymorphism information content (PIC) value were calculated. Phylogenetic trees were constructed by UPGMA.The average number of alleles per locus was 11.5, ranging from 7 (SW72) to 16 (SO068). The Slovak White Improved, Landrace and Yorkshire pig breeds showed out high degree of genetic diversity with mean expected heterozygosities of 0.720, 0.697 and 0.705 respectively. Genetic distance ranged from 0.060 between Yorkshire and Slovak White Improved to 0.203 between Landrace and Slovak White Improved breeds.The scatter diagram from principal component displayed genetic differentiation among all three breeds. A Bayesian method was applied for individual assigning testing. On the base of our results the group of Slovak White Improved population was strongly mixed with Yorkshire breed and the group of all Landrace individuals was presented as enclosed population. Keywords

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Variable (dG-dT) ·(dC-dA) sequences in the porcine genome

Cited by 102 publications

References 29 publications

Protein and microsatellite single locus variability in Salmo salar L. (Atlantic salmon)

Protein and microsatellite single locus variability in Salmo salar L. (Atlantic salmon)

(CT)n and (GT)n microsatellites: a new class of genetic markers for Salmo trutta L. (brown trout)

Genetic admixture in pig population observed by microsatellite markers

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