Variable susceptibility and response to estrogenic chemicals in Menidia menidia

Duffy, Tara A.; McElroy, Anne E.; Conover, David O.

doi:10.3354/meps07928

Cited by 26 publications

(29 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also consistent with previous studies showing elevated expression of VTG protein in YOY winter flounder from Jamaica Bay as compared to Shinnecock Bay as well as female biased sex ratios observed in another local fish, the Atlantic silverside (Menidia menidia) from more urban bays around Long Island (Duffy et al, 2009). Straub et al (2004) previously reported elevated VTG expression in pooled samples of adult winter flounder from a more urban area of NJ as compared to a less contaminated site in southern NJ.…”

Section: Measures Of Exposure To Environmental Contaminantssupporting

confidence: 92%

“…Indeed estimates of young-of-the-year (YOY) mortality in two Long Island bays in 2007 and 2008 indicated higher levels than observed in similar environments from adjacent states including NJ, CT, and RI (Yencho et al, in review). Earlier studies on winter flounder from urban bays of Long Island Sound have shown winter flounder to have reduced fitness in areas with high contaminant loadings (Nelson et al, 1991;Perry et al, 1991;Black et al, 1988), and more recent work has indicated YOY winter flounder and other resident species from western Long Island bays show evidence of feminization McElroy et al, 2006;Duffy et al, 2009).…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Spatial patterns in markers of contaminant exposure, glucose and glycogen metabolism, and immunological response in juvenile winter flounder (Pseudoplueronectes americanus)

McElroy

Hice

Frisk

et al. 2015

Comparative Biochemistry and Physiology Part D: Genomics and Pr

View full text Add to dashboard Cite

Section: Measures Of Exposure To Environmental Contaminantssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 97%

Spatial patterns in markers of contaminant exposure, glucose and glycogen metabolism, and immunological response in juvenile winter flounder (Pseudoplueronectes americanus)

McElroy

Hice

Frisk

et al. 2015

Comparative Biochemistry and Physiology Part D: Genomics and Pr

View full text Add to dashboard Cite

“…To begin with, the sex ratio of Menidia species is susceptible to alteration by estrogen exposure during the larval period (Duffy et al 2009). They are part of the U.S. Environmental Protection Agency's Whole Effluent Toxicity Testing Program (USEPA 2002) and atherinids as a phylogenetic group have been shown to be more sensitive to toxicants in comparison to some other species (Clark et al 1985).…”

Section: Introductionmentioning

confidence: 99%

An approach to detecting estrogenic endocrine disruption via choriogenin expression in an estuarine model fish species

2012

View full text Add to dashboard Cite

A large body of work has established a link between endocrine disrupting compounds (EDCs) and a number of abnormalities in fishes. However, most EDC studies use several standard laboratory denizens to assess impacts, so assumptions about sensitivity are primarily based on these few species. Additionally, existing methods rely on obtaining sufficient plasma to measure EDC biomarkers. Our objectives were (a) to establish a new model species for estuarine fishes, (b) to evaluate endocrine impacts with a highly sensitive and specific biomarker, and (c) to develop a method for the analysis of this biomarker in small fish that do not possess sufficient blood plasma for protein measurement. As such, we created a polyclonal antibody (Ab) to the estrogen-responsive proteins chorion (Ch) and choriogenin (Chg) in Menidia beryllina, found throughout coastal North America and already utilized in EPA Whole Effluent Testing. We then validated the Ab by using it to measure the response to aqueous ethinylestradiol (EE2) through the development an ELISA using Menidia whole body homogenate (WBH). Sensitivity of the Ab to Menidia WBH is greater than that of the commercially available option. ELISA sensitivity, with a detection limit of 5 ng/ml and a working range of 22.6-1370.9 ng/ml, is comparable to ELISAs developed to measure plasma Chg. To our knowledge this is the first ELISA method developed for the detection of Chg using WBH. Including additional model species and methods allowing the evaluation of alternative sample matrices will contribute to an enhanced understanding of inter-species differences in EDC response.

show abstract

“…The adaptive significance of this pattern is that females have more time to grow larger and hence have the ability to carry more eggs [27], [28]. Few EDC studies have been performed with fish that have temperature sensitive sex determination (TSD), but recent findings indicate that Menidia species are highly sensitive to EDCs [29], [30], that exposure may disrupt the adaptive benefits of TSD [31], and that the potential for Menidia species to be widely-utilized North American estuarine bioindicators is unparalleled [29], [32].…”

Section: Introductionmentioning

confidence: 99%

From ‘Omics to Otoliths: Responses of an Estuarine Fish to Endocrine Disrupting Compounds across Biological Scales

Brander

Connon

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

Endocrine disrupting chemicals (EDCs) cause physiological abnormalities and population decline in fishes. However, few studies have linked environmental EDC exposures with responses at multiple tiers of the biological hierarchy, including population-level effects. To this end, we undertook a four-tiered investigation in the impacted San Francisco Bay estuary with the Mississippi silverside (Menidia audens), a small pelagic fish. This approach demonstrated links between different EDC sources and fish responses at different levels of biological organization. First we determined that water from a study site primarily impacted by ranch run-off had only estrogenic activity in vitro, while water sampled from a site receiving a combination of urban, limited ranch run-off, and treated wastewater effluent had both estrogenic and androgenic activity. Secondly, at the molecular level we found that fish had higher mRNA levels for estrogen-responsive genes at the site where only estrogenic activity was detected but relatively lower expression levels where both estrogenic and androgenic EDCs were detected. Thirdly, at the organism level, males at the site exposed to both estrogens and androgens had significantly lower mean gonadal somatic indices, significantly higher incidence of severe testicular necrosis and altered somatic growth relative to the site where only estrogens were detected. Finally, at the population level, the sex ratio was significantly skewed towards males at the site with measured androgenic and estrogenic activity. Our results suggest that mixtures of androgenic and estrogenic EDCs have antagonistic and potentially additive effects depending on the biological scale being assessed, and that mixtures containing androgens and estrogens may produce unexpected effects. In summary, evaluating EDC response at multiple tiers is necessary to determine the source of disruption (lowest scale, i.e. cell line) and what the ecological impact will be (largest scale, i.e. sex ratio).

show abstract

Variable susceptibility and response to estrogenic chemicals in Menidia menidia

Cited by 26 publications

References 55 publications

Spatial patterns in markers of contaminant exposure, glucose and glycogen metabolism, and immunological response in juvenile winter flounder (Pseudoplueronectes americanus)

Spatial patterns in markers of contaminant exposure, glucose and glycogen metabolism, and immunological response in juvenile winter flounder (Pseudoplueronectes americanus)

An approach to detecting estrogenic endocrine disruption via choriogenin expression in an estuarine model fish species

From ‘Omics to Otoliths: Responses of an Estuarine Fish to Endocrine Disrupting Compounds across Biological Scales

Contact Info

Product

Resources

About