Brazil has made progress in innovation, although unevenly across its regions, with the state of Acre still facing challenges in this sector. The creation of an innovation and technology hub at Senai/AC aims to promote regional development by bringing together government, companies, and academia to stimulate local economic growth. This article proposes a strategic framework for the implementation of the Senai/AC Innovation and Technology Hub, with the goal of strengthening the regional economy and increasing industrial competitiveness. Based on a Systematic Literature Review, the best global and regional practices for the creation of innovation ecosystems were identified. The qualitative analysis of 40 articles resulted in strategic guidelines adapted to local needs, highlighting the importance of effective governance, resource mobilization, collaborative environments, human capital development, and a culture of innovation. The Senai/AC Hub has the potential to act as a catalyst for the state's socioeconomic development and strengthen local industries. Future studies can assess the effectiveness of the guidelines and the hub's impact on regional production chains, comparing it with other hubs to identify synergies and best practices.