Variance in resistance to natural and antibody‐dependent cellular cytotoxicity and to complement‐mediated lysis among K562 lines

Kimber, Ian; Moore, Michael G.; Roberts, Kevan

doi:10.1002/ijc.2910320213

Cited by 4 publications

(9 citation statements)

References 38 publications

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“…Besides reduced osmotic fragility [3], which was observed neither with ASML cells nor with K562 variants [23], an active involvement of the target cell membrane during the lethal hit phase has to be considered [17,31]. Besides reduced osmotic fragility [3], which was observed neither with ASML cells nor with K562 variants [23], an active involvement of the target cell membrane during the lethal hit phase has to be considered [17,31].…”

Section: Discussionmentioning

confidence: 99%

Natural cytotoxicity in lymphatic metastasis

Matzku

Oberneder

Keller

et al. 1984

Cancer Immunol Immunother

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Serial transplantation of primary BSp73 ascites cells to a subcutaneous (SC) site gave rise to the appearance of two solid variants differing in their capacity to metastasize via the lymphatics. Tissue cultures derived from variant AS (nonmetastasizing) showed epithelioid morphology, while cultures derived from variant ASML (metastatic) showed spherical morphology. Upon cloning, both variants proved to be operationally homogeneous. Susceptibility of cultured BSp73 cells to NK and macrophage-mediated cytotoxicity was closely correlated to morphology, inasmuch as epithelioid cells were susceptible, while spherical cells were resistant, to lysis. With stimulated effector cells a general increase in cytotoxicity was observed, but epithelioid cells still showed a higher susceptibility level. Resistance of ASML-type cells to natural cytotoxicity was not due to the lack of recognition structures or to a general increase in the mechanical stability of spherical cells. This was concluded from cold target inhibition and from hypotonic shock treatment experiments, respectively.

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Section: Discussionmentioning

confidence: 99%

Natural cytotoxicity in lymphatic metastasis

Matzku

Oberneder

Keller

et al. 1984

Cancer Immunol Immunother

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“…Two cloned populations, designated E10ff2 and F9ff2, were derived from parental K562 by limiting dilution as previously described (Kimber et al, 1983) and maintained in continuous culture in RPMI-1640 supplemented with 10 % heat-inactivated calf serum (Flow Laboratories, Irvine, Scotland) (RPMI-CS).…”

Section: Target Cell Linesmentioning

confidence: 99%

“…The K562 cell line was originally established from the pleural effusion of a patient with chronic myelogenous leukaemia (Lozzio and Lozzio, 1975) and subsequently characterized as erythroleukaemic in nature (Andersson et al, 1979). Two cloned populations, designated E10ff2 and F9ff2, were derived from parental K562 by limiting dilution as previously described (Kimber et al, 1983) and maintained in continuous culture in RPMI-1640 supplemented with 10 % heat-inactivated calf serum (Flow Laboratories, Irvine, Scotland) (RPMI-CS).…”

Section: Target Cell Linesmentioning

confidence: 99%

“…Periodic screening of antibiotic-free cultures confirmed the absence of mycoplasma contamination. As previously demonstrated, these clones exhibit marked and stable differences in their sensitivity to natural cytotoxicity, with E10P2 possessing a susceptible and F9/P2 a resistant phenotype (Kimber et al, 1983). Subsequent recloning of both lines has yielded sub-clones which in each case exhibit susceptibility patterns equivalent to those of the parental population (data not presented).…”

Section: Characterization Of K562 Clonesmentioning

confidence: 99%

“…To further elucidate the effect of cellular differentiation on target cells we have examined the influence of 12-0-tetradecanoylphorbol-13-acetate (TPA) on the sensitivity to lysis of two K562 clones which, as we have previously reported, exhibit marked and stable differences in their capacity to resist natural cytotoxicity (Kimber et al, 1983). We demonstrate that following exposure to P A these clones undergo rapid, reversible but opposite changes in their susceptibility to NK cell lysis.…”

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confidence: 99%

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Influence of 12‐o‐tetradecanoylphorbol‐13‐acetate (TPA) on the susceptibility of K562 to natural cytotoxicity: Evidence for clonal variation in differentiation‐induced changes of lytic sensitivity

Kimber

Moore

Harrison

1984

Intl Journal of Cancer

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The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the sensitivity to NK cell-mediated lysis of two cloned populations of K562 which exhibit marked and stable differences in their susceptibility to natural cytotoxicity has been examined. Culture in medium supplemented with TPA concentrations of l ng/ml or more invariably caused a decrease in the susceptibility of the sensitive clone E10/P2, whereas treatment of the relatively resistant clone F9/P2 with TPA under identical conditions caused a significant increase in susceptibility to natural cytotoxicity. In both cases the change in susceptibility occurred within 1 day of culture in TPA and was rapidly reversible following removal of the inducing agent. The changes in resistance to natural cytotoxicity induced by TPA were independent of variations in osmotic fragility and were not attributable to alterations in NK cell binding capacity as determined by cold competition analysis. In contrast to the effect of TPA, exposure of E10/P2 and F9/P2 to interferon (IFN) caused a reduction in sensitivity to natural cytotoxicity of both populations which was associated with a decreased capacity to compete for lysis of labelled target cells. These data suggest that the effects of differentiating agents on target susceptibility to NK cell lysis are variable and that responses to TPA are clonally distributed within cell populations.

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Natural killer cell resistance in K‐562 cell sublines

Foster

Wust

Lozzio

et al. 1985

Intl Journal of Cancer

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Sublines of the hematopoietic stem cell line K-562 were tested for their susceptibility to human natural killer (NK) cell activity. A correlation was found between the degree of NK-mediated lysis and the presence or absence of particular chromosomal markers. K-562 subline susceptible to lysis by NK were found to express karyotypically a deletion 9- and a marker 8(t1-18), whereas resistant sublines did not express these markers. A cloned K-562 subline B1V was chosen as representative of a resistant subline. This subline was resistant to lysis even after prolonged incubation and activation of the NK cells with interferon. However, it was found that B1V was lysed by both antibody-dependent, complement-mediated and antibody-dependent cellular mechanisms at levels comparable to those seen with NK-sensitive K-562 sublines. Subline B1V did compete poorly with NK-sensitive K-562 in cold-target inhibition; however, conjugate-formation assays demonstrated that the binding of NK cells to B1V cells is comparable to that of NK-sensitive K-562 cells. We suggest that cells of the cloned line B1V are recognized normally by NK cells but do not activate the lytic mechanism of the bound NK cell. Treatment of the resistant clone B1V with neuraminidase did not lead to enhanced levels of lysis. Protein extracts of NK-sensitive K-562 sublines efficiently inhibited lysis by NK cells but extracts of the resistant clone, B1V, did not inhibit lysis, suggesting that the clone lacks cell surface determinants involved in the post-recognitive activation of the NK cytolytic mechanism.

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Variance in resistance to natural and antibody‐dependent cellular cytotoxicity and to complement‐mediated lysis among K562 lines

Cited by 4 publications

References 38 publications

Natural cytotoxicity in lymphatic metastasis

Natural cytotoxicity in lymphatic metastasis

Influence of 12‐o‐tetradecanoylphorbol‐13‐acetate (TPA) on the susceptibility of K562 to natural cytotoxicity: Evidence for clonal variation in differentiation‐induced changes of lytic sensitivity

Natural killer cell resistance in K‐562 cell sublines

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