Variasi    Genetik    Berdasarkan    Penanda    Molekular    Random    Amplified  Polymorphic DNA Pada Jamur Shiitake (Lentinula edodes)

Ekowati, Nuraeni; Kasiamdari, Rina Sri; Pusposendjojo, Nursamsi; Soegihardjo, C.J.

doi:10.24002/biota.v16i2.97

Cited by 3 publications

(2 citation statements)

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“…This dendrogram supposed to be able to separate the Lysurus in species. The dendrogram that built by morphological data were also studied by Khan et al (2011) and Ekowati et al (2011) to separated Pleurotus species. Based on Khan et al (2011), the comparison between the dendrogram built by morphological data and molecular data was Jurnal Biodjati 6(1):102-110, May 2021 http://journal.uinsgd.ac.id/index.php/biodjati not really different for the branching and pattern of the species distribution.…”

Section: Phenetic Relationship Of L Periphragmoides With Other Members Of Lysurusmentioning

confidence: 99%

Morphological characteristic and Phenetic Relationship of Lysurus periphragmoides Collected from West Java

et al. 2021

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Lysurus is one of the unique genera in Basidiomycetes. It has a stinky odor and slime on the head. The unusual-shaped makes the species in Lysurus easily to be identified. One of Lysurus had been found in West Java, Indonesia namely L. periphragmoides. The specimen was deposited into Herbarium Bogoriense with code BO 24418. This study aimed to obtain specimens and characterize the Lysurus BO 24418 using morphological characteristics, and analyze the phenetic relationship among Lysurus species. Lysurus BO 24418 has two phases (egg and mushroom). The egg phase is usually hypogeous underground. The mushroom has a head and stem. The head bears the mature spore with slime distribute malodor. The stem has a hollow and spongy texture with a yellowish color. The numerical data of morphological characters of species in Lysurus were analyzed using NTSys ver 2.1 software. Ten characters were used to build a dendrogram using Sequential Agglomerative Hierarchical Nested (SAHN) cluster analysis with Unweighted Pair Group Method with Arithmetic Mean (UPGMA). Phallus indusiatus was selected as an outgroup. The analyses showed the specimen was classified as L. periphragmoides with 100% of similarity coefficient and it was close L. gardneri with 40,4% of similarity coefficient. The characters that cluster among them are stem surface, head type, egg diameter, and spore max length

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Section: Phenetic Relationship Of L Periphragmoides With Other Members Of Lysurusmentioning

confidence: 99%

Morphological characteristic and Phenetic Relationship of Lysurus periphragmoides Collected from West Java

et al. 2021

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“…Mycelia were harvested using Whatman filter paper no. 41 in a Büchner funnel and used for DNA isolation (Ekowati et al 2011).…”

Section: Dna Extractionmentioning

confidence: 99%

Genetic diversity of Pleurotus ostreatus (Jacq.) P. Kumm. strains in Java based on random amplified polymorphic DNA markers

et al. 2021

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Abstract. Ekowati N, Mumpuni A, Muljowati JS, Ratnaningtyas NI, Maharning AR. 2021. Genetic diversity of Pleurotus ostreatus (Jacq.) P. Kumm. strains in Java based on Random Amplified Polymorphic DNA markers. Biodiversitas 22: 3488-3493. Genetic variation in a fungal population can occur due to mutation and recombination, resulting in changes in the nucleotides that encode specific DNA sequences. Strains with a high genetic distance and good production capabilities can be used to develop genetic breeding. This study aimed to investigate genetic relationship among Pleurotus ostreatus strains cultivated in Java (Bogor, Cianjur, Tasikmalaya, Purwokerto, Yogyakarta, Tawangmangu, Malang, and Madiun) based on random amplified polymorphic DNA (RAPD) markers. The research method consisted of DNA isolation and DNA amplification using six primers, i.e. OPA2, OPA3, OPA4, OPA7, OPA9, and OPA10. DNA band data were analyzed using NTSYSpc21 software to determine the level of genetic similarity, based on the Unweighted Pair Group Method with Arithmetic Average Algorithm (UPGMA). In all, 101 amplified DNA bands were obtained, with sizes ranging from 136 to 2320 bp and 96.0% of the bands were polymorphic. Based on cluster analysis, it shows that three clusters were formed. There were genetic variations and relationships among eight P. ostreatus strains in Java with a genetic similarity varying from 37-98%.

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Characterization and bioactivity test of secondary metabolite isolated from ethyl acetic fraction of Petrosia alfiani sponge from Barrang Lompo Island

Rahmayanti¹,

Ahmad²

2020

The 5th International Conference on Industrial, Mechanical, Electrical, and Chemical Engineering 2019 (Icimece 2019)

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Variasi Genetik Berdasarkan Penanda Molekular Random Amplified Polymorphic DNA Pada Jamur Shiitake (Lentinula edodes)

Cited by 3 publications

References 0 publications

Morphological characteristic and Phenetic Relationship of Lysurus periphragmoides Collected from West Java

Morphological characteristic and Phenetic Relationship of Lysurus periphragmoides Collected from West Java

Genetic diversity of Pleurotus ostreatus (Jacq.) P. Kumm. strains in Java based on random amplified polymorphic DNA markers

Characterization and bioactivity test of secondary metabolite isolated from ethyl acetic fraction of Petrosia alfiani sponge from Barrang Lompo Island

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