2016
DOI: 10.21082/blpn.v16n2.2010.p134-139
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Variasi Genetik Jeruk Keprok SoE (Citrus reticulata Blanco) Hasil Radiasi Sinar Gamma Menggunakan Penanda ISSR

Abstract: <p>Genetic Variation of Keprok SoE Mandarin (Citrus reticulata Blanco) Resulted from Gamma Iradiation Based on ISSR Markers. SoE mandarin is one of the best mandarin from Indonesia which has been chosen as one of mandarin for import substitution. The citrus quality could be improved through breeding program, one of this program was mutation breeding using gamma rays irradiation. The research was aimed to obtain information of SoE mandarin genetic variation derived from gamma rays irradiation using ISSR m… Show more

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Cited by 3 publications
(5 citation statements)
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“…The most effective primer was ISSR_21 and the worst was ISSR_62. These results indicate that the gamma-ray irradiation was the effective method to induce mutations in plants, as previously proved [2,8,14,23]. High PIC, EMR The genetic similarity based on Jaccards scores of the 'Earlibrite' strawberry mutant genotypes, ranged from 3% to 87.04%.…”
Section: Resultssupporting
confidence: 64%
See 1 more Smart Citation
“…The most effective primer was ISSR_21 and the worst was ISSR_62. These results indicate that the gamma-ray irradiation was the effective method to induce mutations in plants, as previously proved [2,8,14,23]. High PIC, EMR The genetic similarity based on Jaccards scores of the 'Earlibrite' strawberry mutant genotypes, ranged from 3% to 87.04%.…”
Section: Resultssupporting
confidence: 64%
“…Even at low doses, gamma-ray irradiation induced nuclear mutation in plants [13]. Mutation in the plant can be detected molecularly using repetitive sequence marker, like ISSR as is done in chrysanthemums [2,8], citrus [14], strawberries [3] and grape [15]. This study aimed to identify the DNA polymorphism of 'Earlibrite' strawberry mutants using ISSR markers.…”
Section: Introductionmentioning
confidence: 99%
“…This gamma ray has the ability to penetrate in deep to plant tissue. Mutation induction was directed to alter one or more important characters while other original characters are retained [3].…”
Section: Introductionmentioning
confidence: 99%
“…Deteksi adanya perubahan dalam susunan gen menggunakan marka molekuler ISSR yang berbasis PCR. Marka ISSR lebih sesuai digunakan untuk tanaman jeruk, karena tidak dipengaruhi oleh musim dan lingkungan (Azrai, 2005), tidak diperlukan data sekuen awal (Guo et al, 2009), hanya membutuhkan 5-50 mg template DNA per reaksi (Sanjay et al, 2011), tersebar di seluruh genom, menghasilkan pita polimorfik sampai pada tingkat kultivar, bersifat dominan (Kumar, 2009), dapat digunakan untuk analisis keragaman genetik dan analisis kekerabatan (Yulianti et al, 2010). Dengan demikian, diharapkan dari proses iradiasi gamma pada kalus embriogenik jeruk keprok SoE bisa diperoleh individu-individu tanaman yang berbeda dalam susunan genetiknya.…”
unclassified
“…Marka ISSR telah digunakan dalam penelitian untuk mengetahui kekerabatan jeruk siam dari beberapa sentra produksi di Indonesia (Agisimanto et al, 2007), dan variasi genetik jeruk keprok SoE hasil iradiasi sinar gamma generasi kedua (Yulianti et al, 2010). Tujuan penelitian adalah untuk mendapatkan informasi mengenai keragaman genetik yang terjadi pada mutan harapan jeruk keprok SoE hasil iradiasi sinar gamma berdasarkan morfologi dan marka ISSR.…”
unclassified