Variation and evolution in the karyotype of Lycoris, Amaryllidaceae. IV Intraspecific variation in the karyotype of L. radiata (L'Herit.) Herb. and the origin of this triploid species.

Kurita, Siro

doi:10.1508/cytologia.52.137

Cited by 26 publications

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“…Cytogenetic studies of the species of L. radiata var. radiata have been made by several authors (Nishiyama, 1928;Inariyama 1931Inariyama , 1944Kurita, 1987). In the present study, we determined that the triploid strains of L. radiata var.…”

Section: Discussionmentioning

confidence: 72%

“…pumila which is diploid, having a 2n=2x=22 chromosome constitution. Kurita (1987) conducted an extensive study on the population cytogenetics of the Japanese triploid strains and reported genetic constancy of their karyotypes.…”

Section: Discussionmentioning

confidence: 99%

“…The triploid sterile species of L. radiata var. radiata is not a simple autotriploid Kurita (1987) reported that the genetic constancy at the level of chromosome constitution with the standard karyotype includes two A type satellite chromosomes heterozygously, i.e ., the size of the short arm of one satellite chromosome is smaller than that of the other. In addition, he observed that the number of nucleoli in an interphase nucleus ranges from one to three, and two-thirds of the nuclei have two nucleoli, one large and one small.…”

Section: Discussionmentioning

confidence: 99%

“…Root tips were placed in 0.1% colchicine solution at room temperature for 3 hrs, and then fixed in an alcohol-acetic acid mixture (3:1, v/v) for 24 hrs at 4 ∞ C. After the tips were washed, they were macerated in 1N HCl at 60 ∞ C for 3 min, and then stained with acetocarmine or aceto-orcein solution for 12-24 hrs. The well-stained root tips were squashed by tapping with a toothpick or by pressing with a thumb or a hand roller (Hori and Tsunewaki, 1969;Kurita, 1987, Mukai et al 1990.…”

Section: Chromosome Analysis and Fluorescence In Situ Hybridiazation mentioning

confidence: 99%

“…pumila which is diploid with a chromosome constitution of 2n=2x=22, by the fusion of a haploid gamete with 11 acrocentric chromosomes and a nonreduced gamete with 22 chromosomes from a diploid species. Kurita (1987) conducted an extensive study of the population cytogenetics of triploid strains in Japan by analyzing 519 bulbs from 58 different localities and reported genetic constancy at the level of their chromosome constitution, by providing evidence that the standard karyotype was found in 97.7% of the plants examined. Chung (1999) carried out allozyme analysis in eight Korean populations of L. radiata var.…”

Section: Introductionmentioning

confidence: 99%

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Genetic variations in Lycoris radiata var. radiata in Japan

Hayashi¹,

Saito²,

Mukai

et al. 2005

Genes Genet. Syst.

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The genetic variations of Lycoris radiata var. radiata , a completely sterile triploid from Japan, were examined by comparing the nucleotide sequences of genomic DNA regions in 11 triploid strains sampled from Japan and four triploid strains sampled from China, and in two diploid strains of Lycoris radiata var. pumila , which is endemic to China and fertile. For this purpose, two genes were analyzed, the lectin gene in the nuclear genome and the maturase gene in the chloroplast genome. A clear genetic constancy was observed in their DNA nucleotide sequences. For both genes, completely identical nucleotide sequences were detected in the 11 Japanese and four Chinese triploid strains and also between the two Chinese diploid strains. However, some genetic variations were observed between the Japanese and Chinese triploid strains, and between the triploid and diploid strains. These results are consistent with the findings obtained from previous chromosome karyotype analyses and allozyme analyses. In addition, in our preliminary FISH analysis of the physical mapping of the rRNA gene family, the 18S-5.8S-26S rRNA and 5S rRNA loci were localized on six and four chromosomes, respectively. Regarding the 18S-5.8S-26S rRNA loci, two were associated with two SAT chromosomes. The remaining four were distinguished by having no secondary constriction. Localization of 5S rRNA loci to chromosome spreads revealed three sites on the proximal part of the long arm of three acrocentric chromosomes and one site on the distal part of the long arm of the SAT chromosome; the latter site was juxtaposed to the 18S-5.8S-26S rRNA loci. These findings indicate that L. radiata var. radiata is not a typical autotriploid. The present paper discusses the possible origin of L. radiata var. radiata from a diploid variety of L. radiata var. pumila , based on the molecular cytogenetic analysis and DNA sequence analysis.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Chromosome Analysis and Fluorescence In Situ Hybridiazation mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genetic variations in Lycoris radiata var. radiata in Japan

Hayashi¹,

Saito²,

Mukai

et al. 2005

Genes Genet. Syst.

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Cytogeography and chromosomal variation of the endemic East Asian herb Lycoris radiata

Liu

Meng

Zheng

et al. 2019

Ecology and Evolution

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Information on the spatial distribution of cytotypes and karyotype variation in plants is critical for studies of the origin and evolution of polyploid complexes. Here, the spatial distribution of cytological races and intraspecific variation in the karyotype of Lycoris radiata, an endemic species to East Asia, is investigated. Conventional karyotype analysis methods were used to determine ploidy level and karyotypical characteristics in 2,420 individuals from 114 populations of L. radiata nearly covering the whole distribution areas in China. Of 114 populations studied, 52 (45.61%), 58 (50.88%), and 4 (3.51%) are diploid, triploid, and mixoploid populations, respectively, with 1,224, 1,195, and 1 individuals being diploid, triploid, and tetraploid, respectively. The triploid possesses a much wider distribution range than the diploid, with the former almost occupying the entire range of this complex species in East Asia and the latter distributing in the middle and east regions of China. Triploids tend to occur at high altitudes, and the relationship between the ploidy and altitude is significantly positive but low (r2 = 0.103, p < 0.01). About 98.6% of examined bulbs have a common karyotype consisting of 22 or 33 acrocentric (A) chromosomes. Some aberrant chromosomes which should be generated from A‐type chromosome have been found including metacentrics (m), small metacentrics (m′), and B‐type chromosome. The results can provide a fundamental cytogeographic data for further studies on the evolutionary origins and adaptive divergences of polyploids, especially the triploid, within L. radiata using molecular and/or ecological methods in the future.

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A karyological review of the orders Asparagales and Liliales (Monocotyledonae)

Tamura

1995

Feddes Repertorium

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Karyology of the Asparagales and Liliales is reviewed with references to their morphology, embryology, DNA analyses, and other aspects. Asparagaceae, Ruscaceae, Dracenaceae, and Nolinaceae may share a common ancestor with x = 10, and Tecophilaeaceae, Cyanastraceae, and Ixioliriaceae with x = 12. Tricyrtis (Uvulariaceae) is karyologically similar to Tulipa, Gagea (Liliaceae) and Smilax (Smilacaceae); Clintonia (Uvulariaceae) to Medeola (Liliaceae); Prosartes and Streptopus (Uvulariaceae) to Calochortus (Calochortaceae); Scoliopus (Trilliaceae) to the Medeola‐Clintonia group or Calochortus‐Prosartes‐Streptopus group. The original chromosome size of the Asparagales and Liliales may be estimated as small.

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Variation and evolution in the karyotype of Lycoris, Amaryllidaceae. IV Intraspecific variation in the karyotype of L. radiata (L'Herit.) Herb. and the origin of this triploid species.

Cited by 26 publications

References 0 publications

Genetic variations in Lycoris radiata var. radiata in Japan

Genetic variations in Lycoris radiata var. radiata in Japan

Cytogeography and chromosomal variation of the endemic East Asian herb Lycoris radiata

A karyological review of the orders Asparagales and Liliales (Monocotyledonae)

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