Variation in chemical constituents, in-vitro bioactivity and toxicity profile among different parts of Clerodendrum glandulosum Lindl. (C. colebrookianum Walp.)

Deb, Prashanta Kumar; Khound, Puspanjali; Bhattacharjee, Swarnali; Choudhury, Paramita; Sarma, Himangshu; Devi, Rajlakshmi; Sarkar, Bikash Kanti

doi:10.1016/j.sajb.2021.03.023

Cited by 25 publications

(25 citation statements)

References 48 publications

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“…DPPH radical scavenging assay was carried out according to Deb et al, 2021. A volume of 100 µL of 0.2 mM DPPH solution prepared in methanol (HPLC grade, Merck) was added to 10 µL of test extract (1 mg mL -1 ) and kept in the dark for 30 min at ambient temperature.…”

Section: 1-diphenyl-2-picrylhydrazyl (Dpph) Radical Scavenging Assaymentioning

confidence: 99%

“…ABTS radical scavenging assay was performed according to the earlier reported method [Deb et al, 2021]. ABTS reagent was prepared by mixing 7 mM ABTS solution in methanol with 2.45 mM potassium persulfate and kept for 12-16 hrs.…”

Section: 2′-azino-bis (3-ethylbenzothiazoline) 6-sulfonic Acid (Abts)...mentioning

confidence: 99%

“…FRAP assay was performed according to Deb et al, 2021. Each extract (100 μL) from stock solution (1 mg mL -1 ) was mixed with 250 µL of phosphate buffer (0.2 M, pH 6.6) and 250 µL of 1% potassium ferricyanide and incubated at 50 °C for 30 min followed by addition of 250 µL of 10% trichloroacetic acid to the reaction mixture.…”

Section: Ferric Reducing Antioxidant Power (Frap) Assaymentioning

confidence: 99%

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Seasonal variation of verbascoside as a principal phenolic compound linked with antioxidant potentials of Clerodendrum glandulosum Lindl. leaves

Deb

Sarkar

2021

Natural Product Research

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This study reports the influence of seasonality on the accumulation of verbascoside as a principal phenolic compound in Clerodendrum glandulosum Lindl. leaves along with possible alteration of antioxidant potentials. Leaves were collected during winter (December 2018), spring (February 2019), summer (May 2019), monsoon (July 2019), autumn (October 2019), and extracted with 95% aqueous methanol by cold maceration. The total phenolic content and antioxidant capacities (DPPH, ABTS and FRAP) were estimated by spectrophotometric technique, and verbascoside content was estimated by HPLC-PDA.Results indicate that the leaves collected during summer and winter both exhibited the highest total phenolic content verbascoside accumulation and antioxidant potentials which are significantly different (p<0.05) than other seasons. Correlation studies further demonstrated that the total polyphenol and verbascoside contents were directly proportional to the antioxidant potentials. Thus, the study concludes that winter and summer are best seasons for collecting leaves from this plant to obtain maximum antioxidant potential.

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Section: 1-diphenyl-2-picrylhydrazyl (Dpph) Radical Scavenging Assaymentioning

confidence: 99%

Section: 2′-azino-bis (3-ethylbenzothiazoline) 6-sulfonic Acid (Abts)...mentioning

confidence: 99%

Section: Ferric Reducing Antioxidant Power (Frap) Assaymentioning

confidence: 99%

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Seasonal variation of verbascoside as a principal phenolic compound linked with antioxidant potentials of Clerodendrum glandulosum Lindl. leaves

Deb

Sarkar

2021

Natural Product Research

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“…This evidence confirmed that C. glandulosum leaves are a potential source of phenolic and flavonoid compounds, which also agrees with previous reports. [17,21] Abundance of phenolic and flavonoid compounds further motivated us to test and compare the antioxidant and cytoprotective properties of all these fractions.…”

Section: Resultsmentioning

confidence: 99%

“…[11,14,15] A few important bioactive compounds, such as verbascoside, martynoside, osmanthuside β6, oleanoleic acid, maslinic acid, and stigmasterol have been reported in C. glandulosum leaves. [16,17] However, to the best of our knowledge, chemical profiling of various fractions of C. glandulosum leaf extract and the molecular interactions of the major metabolites responsible for antioxidant and cytoprotective properties has not been conducted yet. Therefore, this study was strategically designed to identify and quantify major phytochemicals in the crude extract and its different fractions using UHPLC-QTOF-MS/MS technique.…”

Section: Introductionmentioning

confidence: 99%

UHPLC‐ESI‐QTOF‐MS/MS Based Identification, Quantification, and Assessment ofin SilicoMolecular Interactions of Major Phytochemicals from Bioactive Fractions ofClerodendrum glandulosumLindl. Leaves

Deb

Shilkar

Sarkar

2022

Chemistry & Biodiversity

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Clerodendrum glandulosum Lindl., is an ethnopharmacologically important species of the North-eastern region of India. Leaves of this plant are consumed as food and traditionally used as medicine to cure hypertension, diabetes, and other metabolic syndromes. This study was designed to explore the antioxidant potential in the Clerodendrum leaves guided by in-vitro activity, which is responsible for the therapeutic benefits. Leaves were extracted with 95 % methanol and further fractionated with solvents of varying polarities (e. g., petroleum ether, ethyl acetate, and butanol). Total phenolic and total flavonoid content of the crude extract/ fractions were measured by Folin-Ciocalteu and AlCl 3 methods, respectively. Crude extract/ fractions were screen for in-vitro antioxidant and cytoprotective activities to determine the most bioactive fraction. Simultaneously, the chemical constituents of these fractions were identified and characterized using UHPLC-ESI-QTOF-MS/MS. Subsequently, major phenolic compounds identified were subjected to in-silico molecular docking with pro-oxidant enzymes to elucidate possible biological functions. Both ethyl acetate and butanol fractions showed the presence of a high concentration of phenolic and flavonoid content along with the best antioxidant and cytoprotective properties compared to all other fractions. Chemical profiling of these active fractions revealed the presence of different phenolic compounds, among which verbascoside was the principal compound. These major phytoconstituents also exhibited strong binding interactions with the crucial amino acid residues of the target proteins, which complemented the in-vitro bioactivities. In conclusion, this study offers structured information on antioxidant phytochemicals present in C. glandulosum leaves, which would be worthwhile for future investigations on the therapeutic properties at the molecular level.

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Optimization of ultrasonic‐assisted extraction and purification of flavonoids with biological activities from Ficus Pumila L.

Wen

Mai

2023

Journal of Chemometrics

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To optimize the extraction process and activity detection of total flavonoids from Ficus Pumila, a Box-Behnken design of response surface methodology was employed to further optimize ultrasonic-assisted extraction (UAE) conditions for F. Pumila stems total flavonoids (FPSTF). The results showed that optimized extraction conditions were ultrasonic power of 240 W, solvent concentration of 61%, ultrasonic time of 61 min, and liquid-solid ratio of 25 mL/g. Under these conditions, the mean experimental value of extraction yield (1.07%) was achieved, which was about 2.14 times that of ethanol leaching extraction. As compared with FPSTF, FPSTF after purification (FPSPTF) had higher purity of 11.09%, which was nearly 4.37 times higher than that of FPSTF(2.54%). As compared with FPSTF, FPSPTF had higher α-glucosidase and α-amylase inhibitory activities as well as DPPHÁ and ABTSÁ + scavenging activities with an IC 50 value of 0.005, 0.008, 0.22, and 0.08 mg/mL, which was 6.00, 16.50, 1.68, and 2.50 times higher than that of FPSTF (0.030, 0.132, 0.37, and 0.20 mg/mL), respectively. FPSPTF from F. Pumila could be considered as a multifunctional bioactive ingredient to be used in anti-hyperglycemic pharmaceutical formulation and antioxidant.

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Variation in chemical constituents, in-vitro bioactivity and toxicity profile among different parts of Clerodendrum glandulosum Lindl. (C. colebrookianum Walp.)

Cited by 25 publications

References 48 publications

Seasonal variation of verbascoside as a principal phenolic compound linked with antioxidant potentials of Clerodendrum glandulosum Lindl. leaves

Seasonal variation of verbascoside as a principal phenolic compound linked with antioxidant potentials of Clerodendrum glandulosum Lindl. leaves

UHPLC‐ESI‐QTOF‐MS/MS Based Identification, Quantification, and Assessment ofin SilicoMolecular Interactions of Major Phytochemicals from Bioactive Fractions ofClerodendrum glandulosumLindl. Leaves

Optimization of ultrasonic‐assisted extraction and purification of flavonoids with biological activities from Ficus Pumila L.

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